Deep muscle-proteomic analysis of freeze-dried human muscle biopsies reveals fiber type-specific adaptations to exercise training.
Adaptation, Physiological
Biomarkers
/ metabolism
Biopsy
Exercise
Freeze Drying
Glucose
/ metabolism
Humans
Mitochondria
/ metabolism
Muscle Fibers, Fast-Twitch
/ metabolism
Muscle Fibers, Skeletal
/ metabolism
Muscle Fibers, Slow-Twitch
/ metabolism
Muscle, Skeletal
/ metabolism
Principal Component Analysis
Proteome
/ metabolism
Proteomics
Journal
Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555
Informations de publication
Date de publication:
12 01 2021
12 01 2021
Historique:
received:
24
04
2019
accepted:
01
12
2020
entrez:
13
1
2021
pubmed:
14
1
2021
medline:
22
1
2021
Statut:
epublish
Résumé
Skeletal muscle conveys several of the health-promoting effects of exercise; yet the underlying mechanisms are not fully elucidated. Studying skeletal muscle is challenging due to its different fiber types and the presence of non-muscle cells. This can be circumvented by isolation of single muscle fibers. Here, we develop a workflow enabling proteomics analysis of pools of isolated muscle fibers from freeze-dried human muscle biopsies. We identify more than 4000 proteins in slow- and fast-twitch muscle fibers. Exercise training alters expression of 237 and 172 proteins in slow- and fast-twitch muscle fibers, respectively. Interestingly, expression levels of secreted proteins and proteins involved in transcription, mitochondrial metabolism, Ca
Identifiants
pubmed: 33436631
doi: 10.1038/s41467-020-20556-8
pii: 10.1038/s41467-020-20556-8
pmc: PMC7803955
doi:
Substances chimiques
Biomarkers
0
Proteome
0
Glucose
IY9XDZ35W2
Types de publication
Clinical Trial
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
304Commentaires et corrections
Type : ErratumIn
Références
Curr Biol. 1997 Feb 1;7(2):R100-3
pubmed: 9081657
Anat Rec. 1977 Oct;189(2):169-75
pubmed: 911042
Mol Cell Proteomics. 2014 Dec;13(12):3698-708
pubmed: 25360005
Skelet Muscle. 2011 Feb 01;1(1):5
pubmed: 21798083
FASEB J. 2018 Apr;32(4):1741-1777
pubmed: 29242278
Ann Neurol. 2017 Feb;81(2):227-239
pubmed: 28009083
Hum Mol Genet. 2014 Jan 1;23(1):209-25
pubmed: 23975679
Immunology. 1990 Sep;71(1):1-9
pubmed: 1698710
J Physiol. 2013 Dec 1;591(23):5823-31
pubmed: 24127618
EMBO Rep. 2015 Mar;16(3):387-95
pubmed: 25643707
Bioinformatics. 2014 Mar 15;30(6):801-7
pubmed: 22321699
Bioinformatics. 2009 Apr 15;25(8):1091-3
pubmed: 19237447
Muscle Nerve. 2005 May;31(5):572-80
pubmed: 15712281
Proteomics. 2009 Nov;9(22):5155-74
pubmed: 19834892
PLoS Biol. 2004 Oct;2(10):e348
pubmed: 15486583
Diabetes. 1995 Sep;44(9):1010-20
pubmed: 7657022
J Physiol. 2014 Mar 15;592(6):1381-95
pubmed: 24469076
Physiol Rev. 2013 Jul;93(3):993-1017
pubmed: 23899560
Nature. 1981 Apr 9;290(5806):457-65
pubmed: 7219534
J Physiol Sci. 2006 Oct;56(5):355-60
pubmed: 17014746
Am J Physiol Endocrinol Metab. 2003 Feb;284(2):E241-58
pubmed: 12531739
Acta Neuropathol. 2016 Sep;132(3):453-73
pubmed: 27393313
Anal Chem. 2012 Mar 20;84(6):2631-7
pubmed: 22324799
FASEB J. 1994 Feb;8(2):174-81
pubmed: 8119488
J Physiol. 2004 Jun 1;557(Pt 2):559-70
pubmed: 15020699
Int J Biochem Cell Biol. 2013 Oct;45(10):2191-9
pubmed: 23702032
Biochem J. 2016 Aug 1;473(15):2295-314
pubmed: 27470593
Anal Biochem. 2004 Mar 1;326(1):13-20
pubmed: 14769330
Physiol Rep. 2015 Jul;3(7):
pubmed: 26152692
Physiol Rep. 2017 Apr;5(7):
pubmed: 28373411
Mol Cell Proteomics. 2013 Jan;12(1):215-27
pubmed: 23115302
Cell Metab. 2013 Feb 5;17(2):162-84
pubmed: 23395166
Nat Rev Endocrinol. 2012 Apr 03;8(8):457-65
pubmed: 22473333
Nat Biotechnol. 2008 Dec;26(12):1367-72
pubmed: 19029910
Cell Metab. 2017 Jul 5;26(1):256-266.e4
pubmed: 28683291
Pflugers Arch. 2001 Oct;443(1):61-6
pubmed: 11692267
Nutrients. 2019 May 14;11(5):
pubmed: 31091754
Physiol Rev. 2008 Jan;88(1):287-332
pubmed: 18195089
J Appl Physiol (1985). 2016 Nov 1;121(5):1074-1086
pubmed: 27633740
J Proteome Res. 2010 Mar 5;9(3):1323-9
pubmed: 20113005
J Rheumatol. 2002 Jun;29(6):1301-7
pubmed: 12064850
Genome Res. 2003 Nov;13(11):2498-504
pubmed: 14597658
J Physiol. 2019 May;597(9):2421-2444
pubmed: 30843602
Nat Rev Mol Cell Biol. 2012 Sep;13(9):566-78
pubmed: 22850819
Diabetes. 2015 Feb;64(2):485-97
pubmed: 25187364
Biochem Biophys Res Commun. 2007 Aug 17;360(1):181-7
pubmed: 17588536
J Physiol. 2017 May 1;595(9):2955-2968
pubmed: 27396440
Front Physiol. 2015 Oct 21;6:283
pubmed: 26557092
Cell Rep. 2017 Jun 13;19(11):2396-2409
pubmed: 28614723
Physiol Rep. 2018 Feb;6(3):
pubmed: 29417745
Mol Metab. 2016 Sep 09;5(11):1083-1091
pubmed: 27818934
Mol Cell Proteomics. 2014 Sep;13(9):2513-26
pubmed: 24942700
Physiol Rev. 2011 Oct;91(4):1447-531
pubmed: 22013216
J Physiol. 2015 Apr 15;593(8):2053-69
pubmed: 25640469
Biochim Biophys Acta. 2004 Jul 6;1673(1-2):45-55
pubmed: 15238248
Mol Cell Proteomics. 2015 Apr;14(4):841-53
pubmed: 25616865
Nature. 2011 Jun 19;476(7360):341-5
pubmed: 21685886
Mol Cell Proteomics. 2014 Jun;13(6):1573-84
pubmed: 24696503
Mol Metab. 2017 Apr 27;6(7):770-779
pubmed: 28702332
Nat Methods. 2016 Sep;13(9):731-40
pubmed: 27348712
Am J Clin Nutr. 2009 Jan;89(1):467S-71S
pubmed: 19056588
Nature. 2016 Sep 14;537(7620):347-55
pubmed: 27629641
Mol Biosyst. 2011 Apr;7(4):1185-96
pubmed: 21246116
Anal Chem. 2003 Feb 1;75(3):663-70
pubmed: 12585499
Cell. 2006 Jul 14;126(1):107-20
pubmed: 16839880
Cell Metab. 2015 Nov 3;22(5):922-35
pubmed: 26437602
Acta Physiol Scand. 1986 Nov;128(3):397-407
pubmed: 3491492
J Physiol. 2019 Jan;597(1):89-103
pubmed: 30325018
Diabetes. 1979 Jan;28 Suppl 1:30-2
pubmed: 761711
J Physiol. 2018 Jan 15;596(2):231-252
pubmed: 28983994
Proteomics. 2011 Apr;11(8):1413-28
pubmed: 21360670
Proc Natl Acad Sci U S A. 2001 Nov 6;98(23):13108-13
pubmed: 11606756