An SPR imaging immunosensor for leptin determination in blood plasma.

Animals Biosensing Techniques Immunoassay Leptin Plasma Rabbits Surface Plasmon Resonance

Journal

Analytical methods : advancing methods and applications
ISSN: 1759-9679
Titre abrégé: Anal Methods
Pays: England
ID NLM: 101519733

Informations de publication

Date de publication:
07 02 2021
Historique:
pubmed: 14 1 2021
medline: 15 5 2021
entrez: 13 1 2021
Statut: ppublish

Résumé

Leptin is a hormone that has a fundamental role in the regulation of feeding and energy balance. A developed specific SPRi immunosensor for leptin may be a new tool for leptin determination in blood plasma. The immunosensor consists of rabbit anti-leptin antibody immobilized on a gold chip via cysteamine linker, using the EDC/NHS protocol. Non-fluidic array SPRi is used for analytical signal formation. Under optimized conditions, the linear response range of the immunosensor covers concentrations from 0.23 to 5 ng mL

Identifiants

DOI: 10.1039/d0ay02047h PMID: 33438698
pubmed: 33438698
doi: 10.1039/d0ay02047h
doi:

Substances chimiques

Leptin 0

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

642-646

Auteurs

Anna Sankiewicz (A)

Faculty of Chemistry, Bioanalysis Laboratory, University of Bialystok, Bialystok, Poland. ania@uwb.edu.pl.

Adam Hermanowicz (A)

Artur Grycz (A)

Zenon Łukaszewski (Z)

Ewa Gorodkiewicz (E)

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Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › An SPR imaging immunosensor for leptin...
questionsmedicales.fr › Techniques d'investigation › Techniques de sonde moléculaire › Techniques de biocapteur › An SPR imaging immunosensor for leptin...
questionsmedicales.fr › Techniques d'investigation › Techniques de sonde moléculaire › Dosage immunologique › An SPR imaging immunosensor for leptin...
questionsmedicales.fr › Facteurs biologiques › Protéines et peptides de signalisation intercellulaire › Adipokines › Leptine › An SPR imaging immunosensor for leptin...
questionsmedicales.fr › Systèmes sanguin et immunitaire › Sang › Plasma sanguin › An SPR imaging immunosensor for leptin...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Lagomorpha › Lapins › An SPR imaging immunosensor for leptin...
questionsmedicales.fr › Techniques d'investigation › Techniques de sonde moléculaire › Techniques de biocapteur › Résonance plasmonique de surface › An SPR imaging immunosensor for leptin...