Studying chromosome biology with single-molecule resolution in Xenopus laevis egg extracts.
Chromosome organisation
DNA repair
DNA replication
Single-molecule techniques
Xenopus laevis
Journal
Essays in biochemistry
ISSN: 1744-1358
Titre abrégé: Essays Biochem
Pays: England
ID NLM: 0043306
Informations de publication
Date de publication:
16 04 2021
16 04 2021
Historique:
received:
12
09
2020
revised:
16
11
2020
accepted:
25
11
2020
pubmed:
14
1
2021
medline:
7
4
2022
entrez:
13
1
2021
Statut:
ppublish
Résumé
Cell-free extracts from Xenopus laevis eggs are a model system for studying chromosome biology. Xenopus egg extracts can be synchronised in different cell cycle stages, making them useful for studying DNA replication, DNA repair and chromosome organisation. Combining single-molecule approaches with egg extracts is an exciting development being used to reveal molecular mechanisms that are difficult to study using conventional approaches. Fluorescence-based single-molecule imaging of surface-tethered DNAs has been used to visualise labelled protein movements on stretched DNA, the dynamics of DNA-protein complexes and extract-dependent structural rearrangement of stained DNA. Force-based single-molecule techniques are an alternative approach to measure mechanics of DNA and proteins. In this essay, the details of these single-molecule techniques, and the insights into chromosome biology they provide, will be discussed.
Identifiants
pubmed: 33438722
pii: 227578
doi: 10.1042/EBC20200026
pmc: PMC8056035
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
17-26Subventions
Organisme : Cancer Research UK
ID : FC001221
Pays : United Kingdom
Organisme : Medical Research Council
ID : FC001221
Pays : United Kingdom
Organisme : Wellcome Trust
ID : FC001221
Pays : United Kingdom
Informations de copyright
© 2021 The Author(s).
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