The C-terminal Domain of piggyBac Transposase Is Not Required for DNA Transposition.
DNA cleavage
insertion preference
neuron
transposon
vertebrate
Journal
Journal of molecular biology
ISSN: 1089-8638
Titre abrégé: J Mol Biol
Pays: Netherlands
ID NLM: 2985088R
Informations de publication
Date de publication:
02 04 2021
02 04 2021
Historique:
received:
26
11
2020
accepted:
29
12
2020
pubmed:
16
1
2021
medline:
17
6
2021
entrez:
15
1
2021
Statut:
ppublish
Résumé
PiggyBac(PB)-like elements (pble) are members of a eukaryotic DNA transposon family. This family is of interest to evolutionary genomics because pble transposases have been domesticated at least 9 times in vertebrates. The amino acid sequence of pble transposases can be split into three regions: an acidic N-terminal domain (~100 aa), a central domain (~400 aa) containing a DD[D/E] catalytic triad, and a cysteine-rich domain (CRD; ~90 aa). Two recent reports suggested that a functional CRD is required for pble transposase activity. Here we found that two CRD-deficient pble transposases, a PB variant and an isoform encoded by the domesticated PB-derived vertebrate transposase gene 5 (pgbd5) trigger transposition of the Ifp2 pble. When overexpressed in HeLa cells, these CRD-deficient transposases can insert Ifp2 elements with proper and improper transposon ends, associated with deleterious effects on cells. Finally, we found that mouse CRD-deficient transposase Pgbd5, as well as PB, do not insert pbles at random into chromosomes. Transposition events occurred more often in genic regions, in the neighbourhood of the transcription start sites and were often found in genes predominantly expressed in the human central nervous system.
Identifiants
pubmed: 33450253
pii: S0022-2836(20)30730-0
doi: 10.1016/j.jmb.2020.166805
pmc: PMC8426422
mid: NIHMS1730013
pii:
doi:
Substances chimiques
DNA Transposable Elements
0
Nerve Tissue Proteins
0
PGBD1 protein, human
0
PGBD5 protein, human
EC 2.7.7.-
Transposases
EC 2.7.7.-
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
166805Subventions
Organisme : NCI NIH HHS
ID : P30 CA008748
Pays : United States
Organisme : NCI NIH HHS
ID : R01 CA214812
Pays : United States
Commentaires et corrections
Type : ErratumIn
Informations de copyright
Copyright © 2021 Elsevier Ltd. All rights reserved.
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