Morphological study of TNPO3 and SRSF1 interaction during myogenesis by combining confocal, structured illumination and electron microscopy analysis.
Electron microscopy
Myogenesis
SRSF1
Structured illumination microscopy
TNPO3
Journal
Molecular and cellular biochemistry
ISSN: 1573-4919
Titre abrégé: Mol Cell Biochem
Pays: Netherlands
ID NLM: 0364456
Informations de publication
Date de publication:
Apr 2021
Apr 2021
Historique:
received:
14
07
2020
accepted:
11
12
2020
pubmed:
17
1
2021
medline:
15
7
2021
entrez:
16
1
2021
Statut:
ppublish
Résumé
Transportin3 (TNPO3) shuttles the SR proteins from the cytoplasm to the nucleus. The SR family includes essential splicing factors, such as SRSF1, that influence alternative splicing, controlling protein diversity in muscle and satellite cell differentiation. Given the importance of alternative splicing in the myogenic process and in the maintenance of healthy muscle, alterations in the splicing mechanism might contribute to the development of muscle disorders. Combining confocal, structured illumination and electron microscopy, we investigated the expression of TNPO3 and SRSF1 during myogenesis, looking at nuclear and cytoplasmic compartments. We investigated TNPO3 and its interaction with SRSF1 and we observed that SRSF1 remained mainly localized in the nucleus, while TNPO3 decreased in the cytoplasm and was strongly clustered in the nuclei of differentiated myotubes. In conclusion, combining different imaging techniques led us to describe the behavior of TNPO3 and SRSF1 during myogenesis, showing that their dynamics follow the myogenic process and could influence the proteomic network necessary during myogenesis. The combination of different high-, super- and ultra-resolution imaging techniques led us to describe the behavior of TNPO3 and its interaction with SRSF1, looking at nuclear and cytoplasmic compartments. These observations represent a first step in understanding the role of TNPO3 and SRFSF1 in complex mechanisms, such as myogenesis.
Identifiants
pubmed: 33452620
doi: 10.1007/s11010-020-04023-y
pii: 10.1007/s11010-020-04023-y
pmc: PMC7940345
doi:
Substances chimiques
SRSF1 protein, human
0
beta Karyopherins
0
Serine-Arginine Splicing Factors
170974-22-8
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1797-1811Références
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