Validation of a HPLC-UV method for the quantification of budesonide in skin layers.
Budesonide
HPLC-UV
Skin diseases
Skin layers
Topical formulation
Journal
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences
ISSN: 1873-376X
Titre abrégé: J Chromatogr B Analyt Technol Biomed Life Sci
Pays: Netherlands
ID NLM: 101139554
Informations de publication
Date de publication:
01 Feb 2021
01 Feb 2021
Historique:
received:
28
10
2020
revised:
14
12
2020
accepted:
18
12
2020
pubmed:
18
1
2021
medline:
20
5
2021
entrez:
17
1
2021
Statut:
ppublish
Résumé
A simple and sensitive HPLC method for the quantification of budesonide in skin layers was developed and validated. Budesonide was extracted from stratum corneum, epidermis and dermis by means of a mixture of acetonitrile:water (recovery > 90%). Budesonide quantification was performed with a RP-C18 column using methanol and water mixture (69:31, v/v) as mobile phase, pumped at 0.8 ml/min. The absorbance was monitored at 254 nm. The method resulted to be selective, linear in the range 0.05-5 or 10 μg/ml, precise and accurate. LLOQ resulted to be 0.05 μg/ml. The developed method appeared to be appropriate for the quantification of budesonide in skin layers at the end of in vitro permeation experiments since the recovery of the applied dose was 97 ± 1%, in line with requirement of the OECD guideline for the testing of the chemicals (Skin absorption: in vitro method).
Identifiants
pubmed: 33454440
pii: S1570-0232(20)31388-X
doi: 10.1016/j.jchromb.2020.122512
pii:
doi:
Substances chimiques
Budesonide
51333-22-3
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
122512Informations de copyright
Copyright © 2020 Elsevier B.V. All rights reserved.