Physiological effects of inactivation and the roles of Elovl3/ELOVL3 in maintaining ocular homeostasis.
Elovl3
elongase of very long chain fatty acids
lipid homeostasis
meibogenesis
meibomian glands
meibum
transcriptomics
Journal
FASEB journal : official publication of the Federation of American Societies for Experimental Biology
ISSN: 1530-6860
Titre abrégé: FASEB J
Pays: United States
ID NLM: 8804484
Informations de publication
Date de publication:
02 2021
02 2021
Historique:
received:
15
10
2020
revised:
08
12
2020
accepted:
14
12
2020
entrez:
17
1
2021
pubmed:
18
1
2021
medline:
25
6
2021
Statut:
ppublish
Résumé
Recently, elongase of very long chain fatty acids-3 (ELOVL3) was demonstrated to play a pivotal role in physiology and biochemistry of the ocular surface by maintaining a proper balance in the lipid composition of meibum. The goal of this study was to further investigate the effects of ELOVL3 ablation in homozygous Elovl3-knockout mice (E3hom) in comparison with age and sex matched wild-type controls (E3wt). Slit lamp examination of the ocular surface of mice, and histological examination of their ocular tissues, highlighted a severe negative impact of Elovl3 inactivating mutation on the Meibomian glands (MG) and conjunctiva of mice. MG transcriptomes of the E3hom and E3wt mice were assessed and revealed a range of up- and downregulated genes related to lipid biosynthesis, inflammation, and stress response, compared with E3wt mice. Heat stage polarized light microscopy was used to assess melting characteristics of normal and abnormal meibum. The loss of Elovl3 led to a 8°C drop in the melting temperature of meibum in E3hom mice, and increased its fluidity. Also noted were the excessive accumulation of lipid material and tears around the eye and severe ocular inflammation, among other abnormalities.
Identifiants
pubmed: 33455016
doi: 10.1096/fj.202002323R
pmc: PMC7891900
mid: NIHMS1656378
doi:
Substances chimiques
Elovl3 protein, mouse
0
Fatty Acid Elongases
EC 2.3.1.-
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e21327Subventions
Organisme : NEI NIH HHS
ID : R01 EY024324
Pays : United States
Informations de copyright
© 2021 Federation of American Societies for Experimental Biology.
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