Label-Free, Real-Time Phospholipase-A Isoform Assay.

Acute Disease Animals Pancreatitis Phospholipases A2 Phospholipids Protein Isoforms
diether lipids electrical impedance spectroscopy (EIS) ester lipids phospholipase tethered bilayer lipid membranes (tBLMs) venom

Journal

ACS biomaterials science & engineering
ISSN: 2373-9878
Titre abrégé: ACS Biomater Sci Eng
Pays: United States
ID NLM: 101654670

Informations de publication

Date de publication:
10 08 2020
Historique:
entrez: 18 1 2021
pubmed: 19 1 2021
medline: 15 5 2021
Statut: ppublish

Résumé

Phospholipase-A (PLA) enzymes catalyze the hydrolysis of ester bonds in select glycerophospholipids. Sensors for rapidly measuring the PLA activity in biological samples have relevance in the study of venom compositions and in medical diagnostics for the diagnosis of diseases such as acute pancreatitis. Current PLA sensor technologies are often restricted by the time it takes to prepare an assay, the necessity of using fluorescent labels, or the fact they might require strict pH control of the buffer vehicles used. Here we present a tethered bilayer lipid membrane (tBLM) impedance sensor array for the rapid and real-time detection of PLA, which includes the ability to selectively detect phospholipase-A

Identifiants

DOI: 10.1021/acsbiomaterials.0c00632 PMID: 33455186
pubmed: 33455186
doi: 10.1021/acsbiomaterials.0c00632
doi:

Substances chimiques

Phospholipids 0
Protein Isoforms 0
Phospholipases A2 EC 3.1.1.4

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

4714-4721

Auteurs

Alvaro Garcia (A)

School of Life Sciences, University of Technology Sydney, Ultimo, New South Wales 2007, Australia.
ORCID: 0000-0002-1159-4567

Evelyne Deplazes (E)

School of Life Sciences, University of Technology Sydney, Ultimo, New South Wales 2007, Australia.
ORCID: 0000-0003-2052-5536

Samira Aili (S)

School of Life Sciences, University of Technology Sydney, Ultimo, New South Wales 2007, Australia.

Matthew P Padula (MP)

School of Life Sciences, University of Technology Sydney, Ultimo, New South Wales 2007, Australia.
ORCID: 0000-0002-8283-0643

Axel Touchard (A)

CNRS, UMR Ecologie des Forêts de Guyane, AgroParisTech, CIRAD, INRA, Université de Guyane, Université des Antilles, Campus Agronomique, BP316, Kourou Cedex 97379, France.
ORCID: 0000-0002-7766-0088

Christopher Murphy (C)

School of Life Sciences, University of Technology Sydney, Ultimo, New South Wales 2007, Australia.

Upeksha Mirissa Lankage (U)

School of Life Sciences, University of Technology Sydney, Ultimo, New South Wales 2007, Australia.

Graham M Nicholson (GM)

School of Life Sciences, University of Technology Sydney, Ultimo, New South Wales 2007, Australia.
ORCID: 0000-0002-4277-4296

Bruce Cornell (B)

School of Life Sciences, University of Technology Sydney, Ultimo, New South Wales 2007, Australia.
SDx Surgical Diagnostics Pty Ltd., U6 30-32 Barcoo Street, Roseville, New South Wales 2069, Australia.

Charles G Cranfield (CG)

School of Life Sciences, University of Technology Sydney, Ultimo, New South Wales 2007, Australia.
ORCID: 0000-0003-3608-5440

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Classifications MeSH

questionsmedicales.fr › États, signes et symptômes pathologiques › Processus pathologiques › Caractéristiques de la maladie › Maladie aigüe › Label-Free, Real-Time Phospholipase-A Isoform Assay.
questionsmedicales.fr › Eucaryotes › Animaux › Label-Free, Real-Time Phospholipase-A Isoform Assay.
questionsmedicales.fr › Maladies de l'appareil digestif › Maladies du pancréas › Pancréatite › Label-Free, Real-Time Phospholipase-A Isoform Assay.
questionsmedicales.fr › Enzymes et coenzymes › Enzymes › Hydrolases › Esterases › Carboxylic ester hydrolases › Phospholipases A › Phospholipases A2 › Label-Free, Real-Time Phospholipase-A Isoform Assay.
questionsmedicales.fr › Lipides › Lipides membranaires › Phospholipides › Label-Free, Real-Time Phospholipase-A Isoform Assay.
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Isoformes de protéines › Label-Free, Real-Time Phospholipase-A Isoform Assay.