The influence of fixation of biological samples on cell count and marker expression stability in flow cytometric analyses.
Cyto-Chex
Transfix
antigen expression
cerebrospinal fluid
cryopreservation
fixative
flow cytometry
peripheral blood
Journal
Central-European journal of immunology
ISSN: 1426-3912
Titre abrégé: Cent Eur J Immunol
Pays: Poland
ID NLM: 9702239
Informations de publication
Date de publication:
2020
2020
Historique:
received:
26
06
2019
accepted:
11
10
2019
entrez:
18
1
2021
pubmed:
19
1
2021
medline:
19
1
2021
Statut:
ppublish
Résumé
The most common applications of flow cytometry (FC) include diagnostics of haemato-oncological disorders, based on analysis of bone marrow, peripheral blood (PB), or cerebrospinal fluid (CSF) samples. A proper diagnostic process requires standardisation in setting the optimal time frame between material collection and the assay. Unfortunately, this might be difficult to achieve in daily practice due to unintended shipment delays, which might compromise large-scale multicentre studies. Thus, material fixation should be considered as a solution. The most widely used fixative agents are: paraformaldehyde, TransFix
Identifiants
pubmed: 33456333
doi: 10.5114/ceji.2020.95858
pii: 40798
pmc: PMC7792444
doi:
Types de publication
Journal Article
Review
Langues
eng
Pagination
206-213Informations de copyright
Copyright © 2020 Termedia.
Déclaration de conflit d'intérêts
The authors declare no conflict of interest.
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