Isolation and functional analysis of peridroplet mitochondria from murine brown adipose tissue.

Adipocytes, Brown / cytology Adipose Tissue, Brown / cytology Animals Lipid Droplets / metabolism Lipid Metabolism Mice Mitochondria / metabolism
Metabolism Molecular biology

Journal

STAR protocols
ISSN: 2666-1667
Titre abrégé: STAR Protoc
Pays: United States
ID NLM: 101769501

Informations de publication

Date de publication:
19 03 2021
Historique:
entrez: 18 1 2021
pubmed: 19 1 2021
medline: 19 1 2021
Statut: epublish

Résumé

Mitochondria play a central role in lipid metabolism and can bind to lipid droplets. However, the role and functional specialization of the population of peridroplet mitochondria (PDMs) remain unclear, as methods to isolate functional PDMs were not developed until recently. Here, we describe an approach to isolate intact PDMs from murine brown adipose tissue based on their adherence to lipid droplets. PDMs isolated using our approach can be used to study their specialized function by respirometry. For complete information on the use and execution of this protocol, please refer to Benador et al. (2018).

Identifiants

DOI: 10.1016/j.xpro.2020.100243 PMID: 33458705 PMC: PMC7797917
pubmed: 33458705
doi: 10.1016/j.xpro.2020.100243
pii: S2666-1667(20)30230-6
pmc: PMC7797917
doi:

Types de publication

Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

Langues

eng

Pagination

100243

Subventions

Organisme : NIDDK NIH HHS
ID : P30 DK041301
Pays : United States
Organisme : NIAAA NIH HHS
ID : R01 AA026914
Pays : United States
Organisme : NIDDK NIH HHS
ID : R01 DK099618
Pays : United States
Organisme : NIDDK NIH HHS
ID : U54 DK120342
Pays : United States

Informations de copyright

© 2020 The Author(s).

Déclaration de conflit d'intérêts

The authors declare no competing interests.

Références

Cell Metab. 2018 Apr 3;27(4):869-885.e6
pubmed: 29617645
EMBO J. 2020 Jul 1;39(13):e104073
pubmed: 32432379
Curr Protoc Cell Biol. 2020 Dec;89(1):e116
pubmed: 33320426

Auteurs

Jennifer Ngo (J)

Department of Medicine, Division of Endocrinology and Department of Molecular and Medical Pharmacology, David Geffen School of Medicine at UCLA, Los Angeles, CA 90095, USA.
Department of Chemistry and Biochemistry at UCLA, Los Angeles, CA 90095, USA.
Metabolism Theme, David Geffen School of Medicine at UCLA, Los Angeles, CA 90095, USA.

Ilan Y Benador (IY)

Department of Medicine, Division of Endocrinology and Department of Molecular and Medical Pharmacology, David Geffen School of Medicine at UCLA, Los Angeles, CA 90095, USA.

Alexandra J Brownstein (AJ)

Department of Medicine, Division of Endocrinology and Department of Molecular and Medical Pharmacology, David Geffen School of Medicine at UCLA, Los Angeles, CA 90095, USA.
Metabolism Theme, David Geffen School of Medicine at UCLA, Los Angeles, CA 90095, USA.

Laurent Vergnes (L)

Metabolism Theme, David Geffen School of Medicine at UCLA, Los Angeles, CA 90095, USA.
Department of Human Genetics, David Geffen School of Medicine at UCLA, Los Angeles, CA 90024, USA.

Michaela Veliova (M)

Department of Medicine, Division of Endocrinology and Department of Molecular and Medical Pharmacology, David Geffen School of Medicine at UCLA, Los Angeles, CA 90095, USA.
Metabolism Theme, David Geffen School of Medicine at UCLA, Los Angeles, CA 90095, USA.

Michael Shum (M)

Department of Medicine, Division of Endocrinology and Department of Molecular and Medical Pharmacology, David Geffen School of Medicine at UCLA, Los Angeles, CA 90095, USA.
Metabolism Theme, David Geffen School of Medicine at UCLA, Los Angeles, CA 90095, USA.

Rebeca Acín-Pérez (R)

Department of Medicine, Division of Endocrinology and Department of Molecular and Medical Pharmacology, David Geffen School of Medicine at UCLA, Los Angeles, CA 90095, USA.
Metabolism Theme, David Geffen School of Medicine at UCLA, Los Angeles, CA 90095, USA.

Karen Reue (K)

Metabolism Theme, David Geffen School of Medicine at UCLA, Los Angeles, CA 90095, USA.
Department of Human Genetics, David Geffen School of Medicine at UCLA, Los Angeles, CA 90024, USA.
Molecular Biology Institute at UCLA, Los Angeles, CA 90095, USA.

Orian S Shirihai (OS)

Department of Medicine, Division of Endocrinology and Department of Molecular and Medical Pharmacology, David Geffen School of Medicine at UCLA, Los Angeles, CA 90095, USA.
Metabolism Theme, David Geffen School of Medicine at UCLA, Los Angeles, CA 90095, USA.

Marc Liesa (M)

Department of Medicine, Division of Endocrinology and Department of Molecular and Medical Pharmacology, David Geffen School of Medicine at UCLA, Los Angeles, CA 90095, USA.
Metabolism Theme, David Geffen School of Medicine at UCLA, Los Angeles, CA 90095, USA.
Molecular Biology Institute at UCLA, Los Angeles, CA 90095, USA.

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Classifications MeSH

questionsmedicales.fr › Cellules › Cellules du tissu conjonctif › Adipocytes › Adipocytes bruns › Isolation and functional analysis of...
questionsmedicales.fr › Tissus › Tissu conjonctif › Tissu adipeux › Tissu adipeux brun › Isolation and functional analysis of...
questionsmedicales.fr › Eucaryotes › Animaux › Isolation and functional analysis of...
questionsmedicales.fr › Cellules › Structures cellulaires › Espace intracellulaire › Cytoplasme › Structures cytoplasmiques › Organites › Gouttelettes lipidiques › Isolation and functional analysis of...
questionsmedicales.fr › Métabolisme › Métabolisme lipidique › Isolation and functional analysis of...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Rodentia › Muridae › Murinae › Souris › Isolation and functional analysis of...
questionsmedicales.fr › Cellules › Structures cellulaires › Fractions subcellulaires › Mitochondries › Isolation and functional analysis of...