HOXB-AS1 accelerates the tumorigenesis of glioblastoma via modulation of HOBX2 and HOBX3 at transcriptional and posttranscriptional levels.
Animals
Apoptosis
/ genetics
Brain Neoplasms
/ genetics
Carcinogenesis
/ genetics
Cell Line, Tumor
Cell Proliferation
/ genetics
Gene Expression Regulation, Neoplastic
/ genetics
Glioblastoma
/ genetics
Homeodomain Proteins
/ genetics
Humans
Mice
Mice, Nude
MicroRNAs
/ genetics
Nuclear Factor 90 Proteins
/ genetics
RNA Interference
/ physiology
RNA, Antisense
/ genetics
RNA, Long Noncoding
/ genetics
Transcription Factors
/ genetics
Transcription, Genetic
/ genetics
HOXB2
HOXB3
HOXB‐AS1
ILF3
glioblastoma (GBM)
miR‐186‐5p
Journal
Journal of cellular physiology
ISSN: 1097-4652
Titre abrégé: J Cell Physiol
Pays: United States
ID NLM: 0050222
Informations de publication
Date de publication:
01 2021
01 2021
Historique:
received:
25
07
2019
accepted:
09
01
2020
entrez:
18
1
2021
pubmed:
19
1
2021
medline:
31
8
2021
Statut:
ppublish
Résumé
Glioblastoma (GBM) is the most universal and invasive brain tumor among adults. Increasing studies have reported that long noncoding RNAs play vital roles in regulating downstream molecules at the transcriptional or posttranscriptional level in tumor progression. The purpose of the current research was to inquire the modulation mechanism by which homeobox B cluster antisense RNA 1 (HOXB-AS1) functioned in GBM. Our study first discovered the lifted expression of HOXB-AS1 and its nearby genes HOXB2 and HOXB3 in GBM and the positive relationship between HOXB-AS1 and HOXB2 or HOXB3. Loss-of-function assays and in vivo study detected that silencing of HOXB-AS1, HOXB2, or HOXB3 restrained the proliferation and induced the apoptosis in GBM. In addition, mechanism experiments demonstrated that HOXB-AS1 recruited interleukin enhancer-binding factor 3 (ILF3) to regulate HOXB2 and HOXB3 expression at the transcriptional level, and HOXB-AS1 sponged miR-186-5p to modulate HOXB2 and HOXB3 expression at posttranscriptional level. Finally, the regulatory mechanism of HOXB-AS1 in GBM was certified through rescue experiments. Our results indicated that HOXB-AS1 boost the HOXB2 or HOXB3 expression at the transcriptional and posttranscriptional levels. We detected the HOXB-AS1-ILF3-HOXB2/HOXB3 axis and HOXB-AS1-miR-186-5p-HOXB2/HOXB3 axis driving the GBM progression, which might generate more effective diagnostic biomarkers and therapeutic targets for patients with GBM.
Substances chimiques
HOXB2 protein, human
0
Homeodomain Proteins
0
MicroRNAs
0
Nuclear Factor 90 Proteins
0
RNA, Antisense
0
RNA, Long Noncoding
0
Transcription Factors
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
93-106Informations de copyright
© 2020 Wiley Periodicals, Inc.
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