Global and local tension measurements in biomimetic skeletal muscle tissues reveals early mechanical homeostasis.
human
mouse
muscle development
physics of living systems
reconstituted muscle
regenerative medicine
stem cells
tension sensor
Journal
eLife
ISSN: 2050-084X
Titre abrégé: Elife
Pays: England
ID NLM: 101579614
Informations de publication
Date de publication:
18 01 2021
18 01 2021
Historique:
received:
17
06
2020
accepted:
17
01
2021
pubmed:
19
1
2021
medline:
4
2
2022
entrez:
18
1
2021
Statut:
epublish
Résumé
Tension and mechanical properties of muscle tissue are tightly related to proper skeletal muscle function, which makes experimental access to the biomechanics of muscle tissue formation a key requirement to advance our understanding of muscle function and development. Recently developed elastic in vitro culture chambers allow for raising 3D muscle tissue under controlled conditions and to measure global tissue force generation. However, these chambers are inherently incompatible with high-resolution microscopy limiting their usability to global force measurements, and preventing the exploitation of modern fluorescence based investigation methods for live and dynamic measurements. Here, we present a new chamber design pairing global force measurements, quantified from post-deflection, with local tension measurements obtained from elastic hydrogel beads embedded in muscle tissue. High-resolution 3D video microscopy of engineered muscle formation, enabled by the new chamber, shows an early mechanical tissue homeostasis that remains stable in spite of continued myotube maturation.
Identifiants
pubmed: 33459593
doi: 10.7554/eLife.60145
pii: 60145
pmc: PMC7906603
doi:
pii:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Informations de copyright
© 2021, Hofemeier et al.
Déclaration de conflit d'intérêts
AH, TB Patent application filed for the chamber. Application number: LU101799, TL, TM, BW, AJ, MA, ME, RT, NO, JE, PG No competing interests declared
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