Targeting nucleolin by RNA G-quadruplex-forming motif.

Adult Amino Acid Motifs / physiology Cells, Cultured Female G-Quadruplexes Gene Expression Regulation, Neoplastic Gene Targeting / methods Humans Leukocytes, Mononuclear / metabolism Lung Neoplasms / genetics Male Phosphoproteins / biosynthesis RNA-Binding Proteins / biosynthesis Nucleolin
Liquid biopsies Lung Cancer Molecular recognition Nucleolin pre-MIR150 G-quadruplex forming sequence

Journal

Biochemical pharmacology
ISSN: 1873-2968
Titre abrégé: Biochem Pharmacol
Pays: England
ID NLM: 0101032

Informations de publication

Date de publication:
07 2021
Historique:
received: 29 10 2020
revised: 08 01 2021
accepted: 11 01 2021
pubmed: 19 1 2021
medline: 14 9 2021
entrez: 18 1 2021
Statut: ppublish

Résumé

A high level of nucleolin (NCL) expression is often associated with a poor prognosis of patients with lung cancer (LC), suggesting that NCL can be used as a possible biomarker. NCL has been shown to display a marked preference for the binding to G-quadruplexes (G4). Here, we investigate the formation of an RNA quadruplex structure in a sequence found in the human precursor pre-MIR150 with the potential to recognize NCL. Circular dichroism (CD) spectra of pre-MIR150 G4-forming sequence (designated by rG4) indicate the formation of a parallel quadruplex structure in KCl or when complexed with the well-known G4 ligand PhenDC3. The thermal stability of rG4 is very high, and further increases in the presence of PhenDC3. The binding affinities of rG4 to PhenDC3 and NCL RBD1,2 are similar with K

Identifiants

DOI: 10.1016/j.bcp.2021.114418 PMID: 33460627
pubmed: 33460627
pii: S0006-2952(21)00014-9
doi: 10.1016/j.bcp.2021.114418
pii:
doi:

Substances chimiques

Phosphoproteins 0
RNA-Binding Proteins 0

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

114418

Informations de copyright

Copyright © 2021 Elsevier Inc. All rights reserved.

Auteurs

Joana Figueiredo (J)

CICS-UBI - Centro de Investigação em Ciências da Saúde, Universidade da Beira Interior, Av. Infante D. Henrique, Covilhã, Portugal.

André Miranda (A)

CICS-UBI - Centro de Investigação em Ciências da Saúde, Universidade da Beira Interior, Av. Infante D. Henrique, Covilhã, Portugal.

Jéssica Lopes-Nunes (J)

CICS-UBI - Centro de Investigação em Ciências da Saúde, Universidade da Beira Interior, Av. Infante D. Henrique, Covilhã, Portugal.

Josué Carvalho (J)

CICS-UBI - Centro de Investigação em Ciências da Saúde, Universidade da Beira Interior, Av. Infante D. Henrique, Covilhã, Portugal.

Daniela Alexandre (D)

CICS-UBI - Centro de Investigação em Ciências da Saúde, Universidade da Beira Interior, Av. Infante D. Henrique, Covilhã, Portugal.

Salete Valente (S)

Serviço de Pneumologia do Centro Hospitalar Universitário Cova da Beira (CHUCB), Covilhã, Portugal.

Jean-Louis Mergny (JL)

Institute of Biophysics of the CAS, v.v.i., Královopolská 135, 612 65 Brno, Czech Republic; Laboratoire d'Optique et Biosciences, Ecole Polytechnique, CNRS, INSERM, Institut Polytechnique de Paris, 91128 Palaiseau, France.

Carla Cruz (C)

CICS-UBI - Centro de Investigação em Ciências da Saúde, Universidade da Beira Interior, Av. Infante D. Henrique, Covilhã, Portugal. Electronic address: carlacruz@fcsaude.ubi.pt.

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Classifications MeSH

questionsmedicales.fr › Personnes › Tranches d'âge › Adulte › Targeting nucleolin by RNA G-quadruplex-forming motif.
questionsmedicales.fr › Phénomènes chimiques › Phénomènes biochimiques › Conformation moléculaire › Conformation des protéines › Structure secondaire des protéines › Motifs d'acides aminés › Targeting nucleolin by RNA G-quadruplex-forming motif.
questionsmedicales.fr › Cellules › Cellules cultivées › Targeting nucleolin by RNA G-quadruplex-forming motif.
questionsmedicales.fr › Phénomènes génétiques › Structures génétiques › Conformation d'acide nucléique › G-quadruplexes › Targeting nucleolin by RNA G-quadruplex-forming motif.
questionsmedicales.fr › Phénomènes génétiques › Régulation de l'expression des gènes › Régulation de l'expression des gènes tumoraux › Targeting nucleolin by RNA G-quadruplex-forming motif.
questionsmedicales.fr › Techniques d'investigation › Techniques génétiques › Ciblage de gène › Targeting nucleolin by RNA G-quadruplex-forming motif.
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Primates › Haplorhini › Catarrhini › Hominidae › Humains › Targeting nucleolin by RNA G-quadruplex-forming motif.
questionsmedicales.fr › Systèmes sanguin et immunitaire › Système immunitaire › Leucocytes › Agranulocytes › Targeting nucleolin by RNA G-quadruplex-forming motif.
questionsmedicales.fr › Maladies de l'appareil respiratoire › Tumeurs de l'appareil respiratoire › Tumeurs du poumon › Targeting nucleolin by RNA G-quadruplex-forming motif.
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Phosphoprotéines › Targeting nucleolin by RNA G-quadruplex-forming motif.
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Nucléoprotéines › Protéines de liaison à l'ARN › Targeting nucleolin by RNA G-quadruplex-forming motif.