Early patterning of ABCB, ABCC, and ABCG transporters establishes unique territories of small molecule transport in embryonic mesoderm and endoderm.
ATP-Binding Cassette Transporters
/ genetics
Animals
Biological Transport
Endoderm
/ metabolism
Female
Gene Expression Profiling
Gene Expression Regulation, Developmental
In Situ Hybridization
Intestinal Mucosa
/ metabolism
Intestines
/ embryology
Mesoderm
/ metabolism
Sea Urchins
/ embryology
Signal Transduction
ABC transporters
Gut development
MRP
Mesoderm signaling
Sea urchin
Small molecule
Journal
Developmental biology
ISSN: 1095-564X
Titre abrégé: Dev Biol
Pays: United States
ID NLM: 0372762
Informations de publication
Date de publication:
04 2021
04 2021
Historique:
received:
10
07
2020
revised:
08
12
2020
accepted:
22
12
2020
pubmed:
19
1
2021
medline:
14
9
2021
entrez:
18
1
2021
Statut:
ppublish
Résumé
Directed intercellular movement of diverse small molecules, including metabolites, signal molecules and xenobiotics, is a key feature of multicellularity. Networks of small molecule transporters (SMTs), including several ATP Binding Cassette (ABC) transporters, are central to this process. While small molecule transporters are well described in differentiated organs, little is known about their patterns of expression in early embryogenesis. Here we report the pattern of ABC-type SMT expression and activity during the early development of sea urchins. Of the six major ABCs in this embryo (ABCB1, -B4, -C1, -C4, -C5 and -G2), three expression patterns were observed: 1) ABCB1 and ABCC1 are first expressed ubiquitously, and then become enriched in endoderm and ectoderm-derived structures. 2) ABCC4 and ABCC5 are restricted to a ring of mesoderm in the blastula and ABCC4 is later expressed in the coelomic pouches, the embryonic niche of the primordial germ cells. 3) ABCB4 and ABCG2 are expressed exclusively in endoderm-fated cells. Assays with fluorescent substrates and inhibitors of transporters revealed a ring of ABCC4 efflux activity emanating from ABCC4
Identifiants
pubmed: 33460641
pii: S0012-1606(21)00006-3
doi: 10.1016/j.ydbio.2020.12.021
pmc: PMC8171262
mid: NIHMS1703333
pii:
doi:
Substances chimiques
ATP-Binding Cassette Transporters
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, U.S. Gov't, Non-P.H.S.
Langues
eng
Sous-ensembles de citation
IM
Pagination
115-124Subventions
Organisme : NIEHS NIH HHS
ID : F32 ES029843
Pays : United States
Organisme : NIEHS NIH HHS
ID : R01 ES027921
Pays : United States
Organisme : NIEHS NIH HHS
ID : R01 ES030318
Pays : United States
Informations de copyright
Copyright © 2021. Published by Elsevier Inc.
Déclaration de conflit d'intérêts
Declaration of competing interest The authors declare no competing interests.
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