Simultaneous Discovery of Positive and Negative Interactions Among Rhizosphere Bacteria Using Microwell Recovery Arrays.
consortia
high throughput screening
microbial communities
microbial interactions
microbiome
microdevice
microwell
plant growth promoting rhizobacteria
Journal
Frontiers in microbiology
ISSN: 1664-302X
Titre abrégé: Front Microbiol
Pays: Switzerland
ID NLM: 101548977
Informations de publication
Date de publication:
2020
2020
Historique:
received:
01
09
2020
accepted:
07
12
2020
entrez:
20
1
2021
pubmed:
21
1
2021
medline:
21
1
2021
Statut:
epublish
Résumé
Understanding microbe-microbe interactions is critical to predict microbiome function and to construct communities for desired outcomes. Investigation of these interactions poses a significant challenge due to the lack of suitable experimental tools available. Here we present the microwell recovery array (MRA), a new technology platform that screens interactions across a microbiome to uncover higher-order strain combinations that inhibit or promote the function of a focal species. One experimental trial generates 10
Identifiants
pubmed: 33469450
doi: 10.3389/fmicb.2020.601788
pmc: PMC7813777
doi:
Types de publication
Journal Article
Langues
eng
Pagination
601788Informations de copyright
Copyright © 2021 Barua, Herken, Stern, Reese, Powers, Morrell-Falvey, Platt and Hansen.
Déclaration de conflit d'intérêts
RH and TP have filed a patent application on this technology. RP and SR were employed by the company Powers & Zhar. The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
Références
Lab Chip. 2016 Feb 7;16(3):432-46
pubmed: 26758660
Front Microbiol. 2016 Apr 18;7:491
pubmed: 27148182
BMC Bioinformatics. 2016 Apr 19;17:172
pubmed: 27094401
Appl Environ Microbiol. 2016 Aug 30;82(18):5698-708
pubmed: 27422831
Sci Rep. 2019 Sep 24;9(1):13737
pubmed: 31551440
Front Immunol. 2019 Jan 07;9:2868
pubmed: 30666248
Curr Opin Biotechnol. 2014 Jun;27:65-72
pubmed: 24863898
Lab Chip. 2018 Dec 18;19(1):98-110
pubmed: 30488920
PLoS One. 2016 May 06;11(5):e0155080
pubmed: 27152511
Cell Host Microbe. 2014 Jul 9;16(1):94-104
pubmed: 24981331
Evolution. 2020 Jan;74(1):179-187
pubmed: 31393002
Cell. 2012 Mar 16;148(6):1258-70
pubmed: 22424233
Science. 2015 Nov 6;350(6261):663-6
pubmed: 26542567
Proc Natl Acad Sci U S A. 2019 Jun 25;116(26):12804-12809
pubmed: 31186361
Front Microbiol. 2019 Oct 01;10:2163
pubmed: 31632357
Microb Biotechnol. 2017 Jan;10(1):50-53
pubmed: 28074557
Ann Agric Environ Med. 2016 Jun 2;23(2):197-205
pubmed: 27294620
Science. 2011 Sep 23;333(6050):1764-7
pubmed: 21940899
Annu Rev Pathol. 2012;7:99-122
pubmed: 21910623
Nature. 2017 May 31;546(7656):56-64
pubmed: 28569813
mSystems. 2018 Oct 2;3(5):
pubmed: 30320216
Microb Ecol. 2000 Aug;40(2):75-84
pubmed: 11029076
Cold Spring Harb Perspect Med. 2012 Nov 01;2(11):
pubmed: 23125205
New Phytol. 2015 Mar;205(4):1424-30
pubmed: 25422041
Curr Protoc Microbiol. 2012 May;Chapter 3:Unit 3D.3.
pubmed: 22549164
J Vis Exp. 2017 Jun 6;(124):
pubmed: 28654053
Trends Biotechnol. 2019 Feb;37(2):181-197
pubmed: 30497870
J Proteome Res. 2018 Apr 6;17(4):1361-1374
pubmed: 29464956
J R Soc Interface. 2014 Jul 6;11(96):
pubmed: 24829281
Microbiome. 2017 Feb 23;5(1):25
pubmed: 28231859
Chem Soc Rev. 2010 Mar;39(3):1049-59
pubmed: 20179824
ISME J. 2018 Jun;12(6):1520-1531
pubmed: 29588495
ISME J. 2012 Feb;6(2):343-51
pubmed: 21900968
Nat Ecol Evol. 2018 May;2(5):867-872
pubmed: 29662223
Microbiologyopen. 2012 Dec;1(4):438-49
pubmed: 23233458
Front Microbiol. 2018 Mar 20;9:496
pubmed: 29616009
Biotechnol Adv. 2018 Jul - Aug;36(4):1038-1047
pubmed: 29551616
J Vis Exp. 2018 Jun 30;(136):
pubmed: 30010667
Proc Natl Acad Sci U S A. 2006 Mar 7;103(10):3828-33
pubmed: 16537456
Mol Biol Evol. 2017 Sep 1;34(9):2422-2424
pubmed: 28472384
Int J Environ Res Public Health. 2017 Dec 04;14(12):
pubmed: 29207531
Appl Microbiol Biotechnol. 2013 Jul;97(13):5669-79
pubmed: 23681587
Sci Rep. 2016 Jun 30;6:28774
pubmed: 27358031
mBio. 2018 Oct 2;9(5):
pubmed: 30279287
Nucleic Acids Res. 2004 Mar 19;32(5):1792-7
pubmed: 15034147
Syst Biol. 2010 May;59(3):307-21
pubmed: 20525638
Microbiome. 2019 May 18;7(1):76
pubmed: 31103040
Ann Agric Environ Med. 2016 Jun 2;23(2):206-22
pubmed: 27294621
Proc Natl Acad Sci U S A. 2018 Sep 25;115(39):9791-9796
pubmed: 30209211
Nature. 2017 Aug 2;548(7665):43-51
pubmed: 28770836