COVID-19 ARDS is characterized by a dysregulated host response that differs from cytokine storm and is modified by dexamethasone.


Journal

Research square
Titre abrégé: Res Sq
Pays: United States
ID NLM: 101768035

Informations de publication

Date de publication:
14 Jan 2021
Historique:
entrez: 20 1 2021
pubmed: 21 1 2021
medline: 21 1 2021
Statut: epublish

Résumé

We performed comparative lower respiratory tract transcriptional profiling of 52 critically ill patients with the acute respiratory distress syndrome (ARDS) from COVID-19 or from other etiologies, as well as controls without ARDS. In contrast to a cytokine storm, we observed reduced proinflammatory gene expression in COVID-19 ARDS when compared to ARDS due to other causes. COVID-19 ARDS was characterized by a dysregulated host response with increased PTEN signaling and elevated expression of genes with non-canonical roles in inflammation and immunity that were predicted to be modulated by dexamethasone and granulocyte colony stimulating factor. Compared to ARDS due to other types of viral pneumonia, COVID-19 was characterized by impaired interferon-stimulated gene expression (ISG). We found that the relationship between SARS-CoV-2 viral load and expression of ISGs was decoupled in patients with COVID-19 ARDS when compared to patients with mild COVID-19. In summary, assessment of host gene expression in the lower airways of patients with COVID-19 ARDS did not demonstrate cytokine storm but instead revealed a unique and dysregulated host response predicted to be modified by dexamethasone.

Identifiants

pubmed: 33469573
doi: 10.21203/rs.3.rs-141578/v1
pmc: PMC7814832
pii:
doi:

Types de publication

Preprint

Langues

eng

Subventions

Organisme : NHLBI NIH HHS
ID : K23 HL138461
Pays : United States
Organisme : NIAID NIH HHS
ID : T32 AI007641
Pays : United States

Commentaires et corrections

Type : UpdateIn

Références

JCI Insight. 2019 Oct 17;4(20):
pubmed: 31619589
JAMA Intern Med. 2021 Jan 1;181(1):71-78
pubmed: 32910179
Cell Mol Immunol. 2017 Jul;14(7):581-589
pubmed: 28603282
EMBO Mol Med. 2020 Apr 7;12(4):e10128
pubmed: 32176432
F1000Res. 2015 Dec 30;4:1521
pubmed: 26925227
Biochem Biophys Res Commun. 2018 Feb 5;496(2):778-783
pubmed: 29217193
Nat Rev Dis Primers. 2019 Mar 14;5(1):18
pubmed: 30872586
Nat Biotechnol. 2020 Aug;38(8):970-979
pubmed: 32591762
Cell. 2020 May 28;181(5):1036-1045.e9
pubmed: 32416070
Lancet. 2020 Mar 28;395(10229):1033-1034
pubmed: 32192578
Cell Host Microbe. 2020 Jun 10;27(6):992-1000.e3
pubmed: 32320677
J Immunol. 2010 Jul 1;185(1):468-76
pubmed: 20505137
Proc Natl Acad Sci U S A. 2018 Dec 26;115(52):E12353-E12362
pubmed: 30482864
Nature. 2020 Nov;587(7835):619-625
pubmed: 33208946
Genome Biol. 2014;15(12):550
pubmed: 25516281
Nat Biotechnol. 2016 May;34(5):525-7
pubmed: 27043002
Nat Methods. 2016 Jul;13(7):577-80
pubmed: 27240256
Nat Methods. 2015 May;12(5):453-7
pubmed: 25822800
Bioinformatics. 2014 Feb 15;30(4):523-30
pubmed: 24336805
JAKSTAT. 2013 Jan 1;2(1):e24198
pubmed: 24058801
Purinergic Signal. 2016 Dec;12(4):627-635
pubmed: 27421735
Biostatistics. 2017 Apr 1;18(2):275-294
pubmed: 27756721
BMC Biotechnol. 2016 Jun 24;16(1):54
pubmed: 27342544
JCI Insight. 2020 Sep 3;5(17):
pubmed: 32706339
Proc Natl Acad Sci U S A. 2005 Oct 25;102(43):15545-50
pubmed: 16199517
Nat Commun. 2020 Nov 17;11(1):5854
pubmed: 33203890
JAMA. 2012 Jun 20;307(23):2526-33
pubmed: 22797452
Cell. 2020 Jun 25;181(7):1475-1488.e12
pubmed: 32479746
JAMA. 2016 Feb 23;315(8):762-74
pubmed: 26903335
N Engl J Med. 2020 Jul 17;:
pubmed: 32678530
Nat Med. 2020 May;26(5):681-687
pubmed: 32327758
Science. 2020 Aug 7;369(6504):718-724
pubmed: 32661059

Auteurs

Aartik Sarma (A)

Division of Pulmonary, Critical Care, Allergy, and Sleep Medicine, University of California, San Francisco, CA, USA.

Stephanie A Christenson (SA)

Division of Pulmonary, Critical Care, Allergy, and Sleep Medicine, University of California, San Francisco, CA, USA.

Eran Mick (E)

Division of Pulmonary, Critical Care, Allergy, and Sleep Medicine, University of California, San Francisco, CA, USA.
Division of Infectious Diseases, University of California, San Francisco, CA, USA.
Chan Zuckerberg Biohub, San Francisco, CA, USA.

Catherine DeVoe (C)

Division of Infectious Diseases, University of California, San Francisco, CA, USA.

Thomas Deiss (T)

Division of Infectious Diseases, University of California, San Francisco, CA, USA.

Angela Oliveira Pisco (AO)

Chan Zuckerberg Biohub, San Francisco, CA, USA.

Rajani Ghale (R)

Division of Pulmonary, Critical Care, Allergy, and Sleep Medicine, University of California, San Francisco, CA, USA.
Division of Infectious Diseases, University of California, San Francisco, CA, USA.

Alejandra Jauregui (A)

Division of Infectious Diseases, University of California, San Francisco, CA, USA.

Ashley Byrne (A)

Chan Zuckerberg Biohub, San Francisco, CA, USA.

Farzad Moazed (F)

Division of Pulmonary, Critical Care, Allergy, and Sleep Medicine, University of California, San Francisco, CA, USA.

Natasha Spottiswoode (N)

Department of Medicine, University of California, San Francisco, CA, USA.

Pratik Sinha (P)

Department of Anesthesia, Washington University, Saint Louis, Missouri, MO, USA.

Beth Shoshana Zha (BS)

Division of Pulmonary, Critical Care, Allergy, and Sleep Medicine, University of California, San Francisco, CA, USA.

Norma Neff (N)

Chan Zuckerberg Biohub, San Francisco, CA, USA.

Michelle Tan (M)

Chan Zuckerberg Biohub, San Francisco, CA, USA.

Paula Hayakawa Serpa (PH)

Division of Infectious Diseases, University of California, San Francisco, CA, USA.
Chan Zuckerberg Biohub, San Francisco, CA, USA.

K Mark Ansel (KM)

Department of Microbiology and Immunology, University of California, San Francisco, CA, USA.
Sandler Asthma Basic Research Center, University of California, San Francisco, CA, USA.

Jennifer G Wilson (JG)

Department of Emergency Medicine, Stanford University, Palo Alto, CA, USA.

Aleksandra Leligdowicz (A)

Division of Infectious Diseases, University of California, San Francisco, CA, USA.

Emily R Siegel (ER)

School of Medicine, University of California, San Francisco, CA, USA.

Marina Sirota (M)

Division of Rheumatology, University of California, San Francisco, CA, USA.

Joseph L DeRisi (JL)

Chan Zuckerberg Biohub, San Francisco, CA, USA.
Department of Biochemistry and Biophysics, University of California, San Francisco, CA, USA.

Michael A Matthay (MA)

Division of Pulmonary, Critical Care, Allergy, and Sleep Medicine, University of California, San Francisco, CA, USA.

Carolyn M Hendrickson (CM)

Division of Pulmonary, Critical Care, Allergy, and Sleep Medicine, University of California, San Francisco, CA, USA.

Kirsten N Kangelaris (KN)

Department of Medicine, University of California, San Francisco, CA, USA.

Matthew F Krummel (MF)

Department of Pathology, University of California, San Francisco, CA, USA.

Prescott G Woodruff (PG)

Division of Pulmonary, Critical Care, Allergy, and Sleep Medicine, University of California, San Francisco, CA, USA.
Sandler Asthma Basic Research Center, University of California, San Francisco, CA, USA.

David J Erle (DJ)

Department of Medicine, University of California, San Francisco, CA, USA.
Lung Biology Center, University of California, San Francisco, CA, USA.
UCSF CoLabs, University of California, San Francisco, CA, USA.

Carolyn S Calfee (CS)

Division of Pulmonary, Critical Care, Allergy, and Sleep Medicine, University of California, San Francisco, CA, USA.

Charles R Langelier (CR)

Division of Infectious Diseases, University of California, San Francisco, CA, USA.
Chan Zuckerberg Biohub, San Francisco, CA, USA.

Classifications MeSH