Comparison of induced neurons reveals slower structural and functional maturation in humans than in apes.
Chimpanzee
bonobo
developmental biology
evolution
human
neurodevelopment
neuronal function
neuronal maturation
neuroscience
Journal
eLife
ISSN: 2050-084X
Titre abrégé: Elife
Pays: England
ID NLM: 101579614
Informations de publication
Date de publication:
20 01 2021
20 01 2021
Historique:
received:
26
05
2020
accepted:
19
01
2021
pubmed:
21
1
2021
medline:
1
2
2022
entrez:
20
1
2021
Statut:
epublish
Résumé
We generated induced excitatory neurons (iNeurons, iNs) from chimpanzee, bonobo, and human stem cells by expressing the transcription factor neurogenin-2 (NGN2). Single-cell RNA sequencing showed that genes involved in dendrite and synapse development are expressed earlier during iNs maturation in the chimpanzee and bonobo than the human cells. In accordance, during the first 2 weeks of differentiation, chimpanzee and bonobo iNs showed repetitive action potentials and more spontaneous excitatory activity than human iNs, and extended neurites of higher total length. However, the axons of human iNs were slightly longer at 5 weeks of differentiation. The timing of the establishment of neuronal polarity did not differ between the species. Chimpanzee, bonobo, and human neurites eventually reached the same level of structural complexity. Thus, human iNs develop slower than chimpanzee and bonobo iNs, and this difference in timing likely depends on functions downstream of NGN2.
Identifiants
pubmed: 33470930
doi: 10.7554/eLife.59323
pii: 59323
pmc: PMC7870144
doi:
pii:
Types de publication
Comparative Study
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Informations de copyright
© 2021, Schörnig et al.
Déclaration de conflit d'intérêts
MS, XJ, LF, SE, AW, SK, TS, NN, BT, BP, WH, ET No competing interests declared
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