Greater epithelial ridge cells are the principal organoid-forming progenitors of the mouse cochlea.
auditory periphery
epigenetic reprogramming
hair cell regeneration
inner ear organoid
otic
supporting cell
Journal
Cell reports
ISSN: 2211-1247
Titre abrégé: Cell Rep
Pays: United States
ID NLM: 101573691
Informations de publication
Date de publication:
19 01 2021
19 01 2021
Historique:
received:
14
08
2020
revised:
01
11
2020
accepted:
21
12
2020
entrez:
20
1
2021
pubmed:
21
1
2021
medline:
29
1
2022
Statut:
ppublish
Résumé
In mammals, hearing loss is irreversible due to the lack of regenerative potential of non-sensory cochlear cells. Neonatal cochlear cells, however, can grow into organoids that harbor sensory epithelial cells, including hair cells and supporting cells. Here, we purify different cochlear cell types from neonatal mice, validate the composition of the different groups with single-cell RNA sequencing (RNA-seq), and assess the various groups' potential to grow into inner ear organoids. We find that the greater epithelial ridge (GER), a transient cell population that disappears during post-natal cochlear maturation, harbors the most potent organoid-forming cells. We identified three distinct GER cell groups that correlate with a specific spatial distribution of marker genes. Organoid formation was synergistically enhanced when the cells were cultured at increasing density. This effect is not due to diffusible signals but requires direct cell-to-cell contact. Our findings improve the development of cell-based assays to study culture-generated inner ear cell types.
Identifiants
pubmed: 33472062
pii: S2211-1247(20)31635-1
doi: 10.1016/j.celrep.2020.108646
pmc: PMC7847202
mid: NIHMS1664961
pii:
doi:
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
108646Subventions
Organisme : NIDCD NIH HHS
ID : P30 DC010363
Pays : United States
Organisme : NIDCD NIH HHS
ID : R01 DC015201
Pays : United States
Organisme : NIDCD NIH HHS
ID : R01 DC017689
Pays : United States
Organisme : NIDCD NIH HHS
ID : T32 DC015209
Pays : United States
Informations de copyright
Copyright © 2020 The Author(s). Published by Elsevier Inc. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of interests S.H. is a paid consultant of Pipeline Therapeutics.
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