Direct reprogramming of epithelial cell rests of malassez into mesenchymal-like cells by epigenetic agents.

Animals Azacitidine / pharmacology Cell Dedifferentiation / drug effects Cell Survival / drug effects Cellular Reprogramming / drug effects Culture Media / chemistry Epithelial Cells / cytology Mesenchymal Stem Cells / cytology Swine Transcription Factors / genetics Valproic Acid / pharmacology

Journal

Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288

Informations de publication

Date de publication:
20 01 2021
Historique:
received: 24 01 2020
accepted: 04 12 2020
entrez: 21 1 2021
pubmed: 22 1 2021
medline: 14 9 2021
Statut: epublish

Résumé

The DNA demethylating agent, 5-Azacytidine (5Aza), and histone deacetylase inhibitor, valproic acid (Vpa), can improve the reprogramming efficiencies of pluripotent cells. This study aimed to examine the roles of 5Aza and Vpa in the dedifferentiation of epithelial cell rests of Malassez (ERM) into stem-like cells. Additionally, the ability of stem-like cells to differentiate into mesenchymal cells was evaluated. ERM was cultured in embryonic stem cell medium (ESCM) with 1 µM of 5Aza, or 2 mM of Vpa, or a combination of 5Aza and Vpa. The cells stimulated with both 5Aza and Vpa were named as progenitor-dedifferentiated into stem-like cells (Pro-DSLCs). The Pro-DSLCs cultured in ESCM alone for another week were named as DSLCs. The stem cell markers were significantly higher in the DSLCs than the controls (no additions). The mRNA and protein levels of the endothelial, mesenchymal stem, and osteogenic cell markers were significantly higher in the Pro-DSLCs and DSLCs than the controls. The combination of a demethylating agent and a deacetylated inhibitor induced the dedifferentiation of ERM into DSLCs. The Pro-DSLCs derived from ERM can be directly reprogrammed into mesenchymal-like cells without dedifferentiation into stem-like cells. Isolated ERM treated with epigenetic agents may be used for periodontal regeneration.

Identifiants

DOI: 10.1038/s41598-020-79426-4 PMID: 33473142 PMC: PMC7817677
pubmed: 33473142
doi: 10.1038/s41598-020-79426-4
pii: 10.1038/s41598-020-79426-4
pmc: PMC7817677
doi:

Substances chimiques

Culture Media 0
Transcription Factors 0
Valproic Acid 614OI1Z5WI
Azacitidine M801H13NRU

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

1852

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Auteurs

Koki Yoshida (K)

Division of Oral Medicine and Pathology, Department of Human Biology and Pathophysiology, School of Dentistry, Health Sciences University of Hokkaido, 1757 Kanazawa, Ishikari-Tobetsu, Hokkaido, 061-0293, Japan.

Osamu Uehara (O)

Division of Disease Control and Molecular Epidemiology, Department of Oral Growth and Development, School of Dentistry, Health Sciences University of Hokkaido, 1757 Kanazawa, Ishikari-Tobetsu, Hokkaido, 061-0293, Japan.

Yoshihito Kurashige (Y)

Division of Pediatric Dentistry, School of Dentistry, Health Sciences University of Hokkaido, 1757 Kanazawa, Ishikari-Tobetsu, Hokkaido, 061-0293, Japan.

Durga Paudel (D)

Division of Oral Medicine and Pathology, Department of Human Biology and Pathophysiology, School of Dentistry, Health Sciences University of Hokkaido, 1757 Kanazawa, Ishikari-Tobetsu, Hokkaido, 061-0293, Japan.

Aya Onishi (A)

Division of Oral Medicine and Pathology, Department of Human Biology and Pathophysiology, School of Dentistry, Health Sciences University of Hokkaido, 1757 Kanazawa, Ishikari-Tobetsu, Hokkaido, 061-0293, Japan.

Puja Neopane (P)

Division of Oral Medicine and Pathology, Department of Human Biology and Pathophysiology, School of Dentistry, Health Sciences University of Hokkaido, 1757 Kanazawa, Ishikari-Tobetsu, Hokkaido, 061-0293, Japan.

Daichi Hiraki (D)

Division of Reconstructive Surgery for Oral and Maxillofacial Region, Department of Human Biology and Pathophysiology, School of Dentistry, Health Sciences University of Hokkaido, 1757 Kanazawa, Ishikari-Tobetsu, Hokkaido, 061-0293, Japan.

Tetsuro Morikawa (T)

Division of Oral Medicine and Pathology, Department of Human Biology and Pathophysiology, School of Dentistry, Health Sciences University of Hokkaido, 1757 Kanazawa, Ishikari-Tobetsu, Hokkaido, 061-0293, Japan.

Fumiya Harada (F)

Division of Oral and Maxillofacial Surgery, Department of Human Biology and Pathophysiology, School of Dentistry, Health Sciences University of Hokkaido, 1757 Kanazawa, Ishikari-Tobetsu, Hokkaido, 061-0293, Japan.

Rie Takai (R)

Research Institute of Health Sciences, Health Sciences University of Hokkaido, 1757 Kanazawa, Ishikari-Tobetsu, Hokkaido, 061-0293, Japan.

Jun Sato (J)

Division of Oral Medicine and Pathology, Department of Human Biology and Pathophysiology, School of Dentistry, Health Sciences University of Hokkaido, 1757 Kanazawa, Ishikari-Tobetsu, Hokkaido, 061-0293, Japan.

Masato Saitoh (M)

Division of Pediatric Dentistry, School of Dentistry, Health Sciences University of Hokkaido, 1757 Kanazawa, Ishikari-Tobetsu, Hokkaido, 061-0293, Japan.

Yoshihiro Abiko (Y)

Division of Oral Medicine and Pathology, Department of Human Biology and Pathophysiology, School of Dentistry, Health Sciences University of Hokkaido, 1757 Kanazawa, Ishikari-Tobetsu, Hokkaido, 061-0293, Japan. yoshi-ab@hoku-iryo-u.ac.jp.

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Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › Direct reprogramming of epithelial cell rests...
questionsmedicales.fr › Acides nucléiques, nucléotides et nucléosides › Nucléosides › Ribonucléosides › Cytidine › Azacitidine › Direct reprogramming of epithelial cell rests...
questionsmedicales.fr › Phénomènes physiologiques cellulaires › Dédifférenciation cellulaire › Direct reprogramming of epithelial cell rests...
questionsmedicales.fr › Phénomènes physiologiques cellulaires › Survie cellulaire › Direct reprogramming of epithelial cell rests...
questionsmedicales.fr › Phénomènes génétiques › Reprogrammation cellulaire › Direct reprogramming of epithelial cell rests...
questionsmedicales.fr › Équipement et fournitures › Milieux de culture › Direct reprogramming of epithelial cell rests...
questionsmedicales.fr › Cellules › Cellules épithéliales › Direct reprogramming of epithelial cell rests...
questionsmedicales.fr › Cellules › Cellules souches › Cellules souches multipotentes › Cellules souches mésenchymateuses › Direct reprogramming of epithelial cell rests...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Artiodactyla › Suidae › Direct reprogramming of epithelial cell rests...
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questionsmedicales.fr › Lipides › Acides gras › Acides gras volatils › Valérates › Acides pentanoïques › Acide valproïque › Direct reprogramming of epithelial cell rests...