Base-modified aptamers obtained by cell-internalization SELEX facilitate cellular uptake of an antisense oligonucleotide.
MALAT1
SELEX
antisense oligonucleotide
aptamer
artificial nucleic acid
Journal
Molecular therapy. Nucleic acids
ISSN: 2162-2531
Titre abrégé: Mol Ther Nucleic Acids
Pays: United States
ID NLM: 101581621
Informations de publication
Date de publication:
05 Mar 2021
05 Mar 2021
Historique:
received:
08
07
2020
accepted:
20
11
2020
entrez:
21
1
2021
pubmed:
22
1
2021
medline:
22
1
2021
Statut:
epublish
Résumé
Intracellular delivery of oligonucleotides is important for their use as therapeutic drugs. The conjugation of molecules interacting with cell membrane proteins to enhance their internalization into cells is an effective strategy for delivering oligonucleotides. In the present study, we focused on creating aptamers, which are single-stranded oligonucleotides that bind target molecules with high affinity and specificity, as membrane protein-binding molecules. With an evolutionary selection approach using a random DNA library containing a uracil derivative with a hydrophobic functional group at the 5 position, we successfully obtained aptamers that are efficiently internalized into A549 cells. The efficacies of the aptamers were tested by further conjugation with
Identifiants
pubmed: 33473329
doi: 10.1016/j.omtn.2020.11.016
pii: S2162-2531(20)30372-3
pmc: PMC7803630
doi:
Types de publication
Journal Article
Langues
eng
Pagination
440-449Informations de copyright
© 2020 The Authors.
Déclaration de conflit d'intérêts
The authors declare no competing interests.
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