CRISPR-Cas9 gene editing of hepatitis B virus in chronically infected humanized mice.
AAV
CRISPR/Cas9
Gene editing
HBV
Humanized mouse
Journal
Molecular therapy. Methods & clinical development
ISSN: 2329-0501
Titre abrégé: Mol Ther Methods Clin Dev
Pays: United States
ID NLM: 101624857
Informations de publication
Date de publication:
12 Mar 2021
12 Mar 2021
Historique:
received:
17
07
2020
accepted:
18
11
2020
entrez:
21
1
2021
pubmed:
22
1
2021
medline:
22
1
2021
Statut:
epublish
Résumé
Chronic hepatitis B virus (HBV) infection is a major public health problem. New treatment approaches are needed because current treatments do not target covalently closed circular DNA (cccDNA), the template for HBV replication, and rarely clear the virus. We harnessed adeno-associated virus (AAV) vectors and CRISPR-
Identifiants
pubmed: 33473359
doi: 10.1016/j.omtm.2020.11.014
pii: S2329-0501(20)30241-2
pmc: PMC7803634
doi:
Types de publication
Journal Article
Langues
eng
Pagination
258-275Subventions
Organisme : NCI NIH HHS
ID : P30 CA015704
Pays : United States
Organisme : NIAID NIH HHS
ID : R21 AI107252
Pays : United States
Organisme : NIAID NIH HHS
ID : UM1 AI106701
Pays : United States
Organisme : NIAID NIH HHS
ID : UM1 AI126623
Pays : United States
Informations de copyright
© 2020 The Author(s).
Déclaration de conflit d'intérêts
The authors declare no competing interests.
Références
Sci Rep. 2017 Aug 7;7(1):7401
pubmed: 28785016
Nat Commun. 2019 Apr 23;10(1):1842
pubmed: 31015529
Front Microbiol. 2016 Jan 06;6:1491
pubmed: 26779147
Mol Ther. 2020 Jun 3;28(6):1432-1441
pubmed: 32348718
Sci China C Life Sci. 2009 Aug;52(8):719-23
pubmed: 19727589
Mol Ther. 2008 Feb;16(2):280-9
pubmed: 18059373
Hepatology. 2017 Dec;66(6):1750-1765
pubmed: 28445592
PLoS Biol. 2018 Dec 12;16(12):e2005595
pubmed: 30540740
Nature. 2015 Apr 9;520(7546):186-91
pubmed: 25830891
World J Gastroenterol. 2015 Aug 28;21(32):9554-65
pubmed: 26327763
Mol Ther. 2008 Mar;16(3):494-9
pubmed: 18180769
Front Cell Infect Microbiol. 2017 Mar 22;7:91
pubmed: 28382278
PLoS One. 2014 May 14;9(5):e97579
pubmed: 24827459
Lab Invest. 2013 Jan;93(1):54-71
pubmed: 23147226
Hum Gene Ther Methods. 2012 Feb;23(1):18-28
pubmed: 22428977
Proc Natl Acad Sci U S A. 2014 Aug 5;111(31):11461-6
pubmed: 25049410
Proc Natl Acad Sci U S A. 2013 Oct 8;110(41):16538-43
pubmed: 24043801
J Gen Virol. 2015 Aug;96(8):2252-2261
pubmed: 25904148
Hum Genet. 2016 Sep;135(9):1071-82
pubmed: 27272125
Antiviral Res. 2016 Feb;126:90-8
pubmed: 26718067
Sci Rep. 2015 Sep 03;5:13734
pubmed: 26334116
Biochem Biophys Res Commun. 2011 Feb 18;405(3):405-10
pubmed: 21238430
Bioinformatics. 2014 Aug 1;30(15):2114-20
pubmed: 24695404
Antiviral Res. 2018 Apr;152:58-67
pubmed: 29458131
Nat Biotechnol. 2007 Aug;25(8):903-10
pubmed: 17664939
Proc Natl Acad Sci U S A. 2015 Oct 20;112(42):E5715-24
pubmed: 26438841
Hepatology. 2017 Dec;66(6):2066-2077
pubmed: 28833361
Gene Ther. 2016 Aug;23(8-9):690-5
pubmed: 27194423
J Med Virol. 2001 Oct;65(2):257-65
pubmed: 11536231
Nat Biotechnol. 2013 Sep;31(9):827-32
pubmed: 23873081
Hum Gene Ther. 2001 Mar 20;12(5):563-73
pubmed: 11268288
Mol Ther Nucleic Acids. 2014 Dec 16;3:e216
pubmed: 25514649
Mol Ther Nucleic Acids. 2014 Feb 04;3:e146
pubmed: 24496438
Mol Ther. 2013 Oct;21(10):1889-97
pubmed: 23883864
Hum Gene Ther. 2011 Sep;22(9):1143-53
pubmed: 21476867
Biochem Biophys Res Commun. 2013 Nov 8;441(1):230-5
pubmed: 24140055
Nature. 2014 Feb 20;506(7488):382-6
pubmed: 24390344
J Virol. 2012 Sep;86(17):8920-36
pubmed: 22718830
Hepatology. 2005 Apr;41(4):847-56
pubmed: 15791625
Cell Rep. 2016 Apr 19;15(3):481-489
pubmed: 27068471
Antiviral Res. 2015 Jun;118:110-7
pubmed: 25843425
Clin Microbiol Infect. 2016 Mar;22(3):290.e1-3
pubmed: 26585773
Curr Protoc Mol Biol. 2007 Apr;Chapter 16:Unit 16.25
pubmed: 18265393
Microbiol Immunol. 2016 Jul;60(7):483-96
pubmed: 27278725
JCI Insight. 2016 Sep 8;1(14):
pubmed: 27642635
Mol Ther Methods Clin Dev. 2018 Jun 15;10:105-112
pubmed: 30073181
Genome Biol. 2010;11(8):R86
pubmed: 20738864
Nucleic Acids Res. 2013 Jan;41(Database issue):D566-70
pubmed: 23125365
J Clin Pathol. 2008 Mar;61(3):338-42
pubmed: 17693576
Genes Cells. 2016 Nov;21(11):1253-1262
pubmed: 27659023
Hepatology. 2005 Nov;42(5):1046-54
pubmed: 16250045
Nat Biotechnol. 2014 Dec;32(12):1262-7
pubmed: 25184501
Antiviral Res. 2018 Jan;149:41-47
pubmed: 29129708
Gene Ther. 2015 May;22(5):404-12
pubmed: 25652100
Nat Med. 2001 Aug;7(8):927-33
pubmed: 11479625
Nat Genet. 1995 Aug;10(4):453-60
pubmed: 7545495
Hepatology. 2006 Sep;44(3):694-702
pubmed: 16941694
Biotechnol Lett. 2015 Oct;37(10):2063-73
pubmed: 26187315
Mol Ther Nucleic Acids. 2014 Aug 19;3:e186
pubmed: 25137139
Bioinformatics. 2012 Jun 15;28(12):1647-9
pubmed: 22543367
Virology. 2015 Feb;476:196-205
pubmed: 25553515
Mol Ther. 2016 Aug;24(7):1258-66
pubmed: 27203444
Gastroenterology. 2004 Jun;126(7):1750-8
pubmed: 15188170
Bioinformatics. 2009 Oct 1;25(19):2607-8
pubmed: 19654119
Genome Biol. 2004;5(10):R80
pubmed: 15461798
RNA. 2015 Sep;21(9):1683-9
pubmed: 26187160
Nat Methods. 2012 Mar 04;9(4):357-9
pubmed: 22388286
J Gen Virol. 1977 Jul;36(1):59-74
pubmed: 886304
PLoS One. 2016 Jan 14;11(1):e0147007
pubmed: 26765841
Virology. 2014 Apr;454-455:353-61
pubmed: 24485787
Cancer Res. 1982 Sep;42(9):3858-63
pubmed: 6286115
Mol Ther. 2008 Feb;16(2):290-5
pubmed: 18087261
Biotechniques. 1998 May;24(5):760-2
pubmed: 9591124
Virus Res. 2016 Jun 2;217:125-32
pubmed: 27049051
J Clin Invest. 2010 Mar;120(3):924-30
pubmed: 20179355
Nat Biomed Eng. 2019 Oct;3(10):806-816
pubmed: 31332341
J Virol. 2006 Mar;80(6):2797-807
pubmed: 16501088
Intervirology. 2003;46(6):400-7
pubmed: 14688459
Gastroenterology. 2007 Sep;133(3):843-52
pubmed: 17854594
Mol Brain. 2014 Mar 11;7:17
pubmed: 24618276
Mol Ther. 2014 Feb;22(2):303-311
pubmed: 24025750
Protein Eng Des Sel. 2017 Jul 1;30(7):503-522
pubmed: 28873986
Genome Biol. 2015 Nov 24;16:257
pubmed: 26596280
J Hepatol. 2016 Apr;64(1 Suppl):S17-S31
pubmed: 27084033
Gastroenterology. 2018 Feb;154(3):652-662.e8
pubmed: 29079518
Gastroenterology. 2000 Mar;118(3):554-9
pubmed: 10702206
Am J Gastroenterol. 2002 Feb;97(2):406-12
pubmed: 11866280
Int J Biol Sci. 2016 Aug 05;12(9):1104-13
pubmed: 27570484
World J Gastroenterol. 2017 Sep 14;23(34):6252-6260
pubmed: 28974891
Gene Ther. 1999 Jun;6(6):973-85
pubmed: 10455399
Nat Med. 2019 Feb;25(2):249-254
pubmed: 30692695
Mol Ther. 2016 Mar;24(3):522-6
pubmed: 26796669
Sci Rep. 2015 Jun 02;5:10833
pubmed: 26035283
Nat Methods. 2009 May;6(5):343-5
pubmed: 19363495
Nucleic Acids Res. 2017 Nov 16;45(20):12039-12053
pubmed: 29036671
Nat Commun. 2020 Aug 18;11(1):4148
pubmed: 32811834
PLoS One. 2015 Feb 03;10(2):e0117741
pubmed: 25647607
Mol Ther. 2010 May;18(5):947-54
pubmed: 20160705
Mol Ther. 2017 May 3;25(5):1168-1186
pubmed: 28366764
Front Immunol. 2018 Sep 11;9:2080
pubmed: 30254645
Nat Med. 2019 Feb;25(2):242-248
pubmed: 30374197