Unveiling the polarity of actin filaments by cryo-electron tomography.
actin
actin filaments
cell adhesion
cryo-electron tomography
focal adhesions
Journal
Structure (London, England : 1993)
ISSN: 1878-4186
Titre abrégé: Structure
Pays: United States
ID NLM: 101087697
Informations de publication
Date de publication:
06 05 2021
06 05 2021
Historique:
received:
18
08
2020
revised:
04
12
2020
accepted:
23
12
2020
pubmed:
22
1
2021
medline:
30
11
2021
entrez:
21
1
2021
Statut:
ppublish
Résumé
The actin cytoskeleton plays a fundamental role in numerous cellular processes, such as cell motility, cytokinesis, and adhesion to the extracellular matrix. Revealing the polarity of individual actin filaments in intact cells would foster an unprecedented understanding of cytoskeletal processes and their associated mechanical forces. Cryo-electron tomography provides the means for high-resolution structural imaging of cells. However, the low signal-to-noise ratio of cryo-tomograms obscures the high frequencies, and therefore the polarity of actin filaments cannot be directly measured. Here, we developed a method that enables us to determine the polarity of actin filaments in cellular cryo-tomograms. We applied it to reveal the actin polarity distribution in focal adhesions, and show a linear relation between actin polarity and distance from the apical boundary of the adhesion site.
Identifiants
pubmed: 33476550
pii: S0969-2126(20)30481-0
doi: 10.1016/j.str.2020.12.014
pmc: PMC8111420
pii:
doi:
Substances chimiques
Actins
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
488-498.e4Informations de copyright
Copyright © 2020 The Authors. Published by Elsevier Ltd.. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of interests The authors have no conflicts of interest to declare.
Références
Proc Natl Acad Sci U S A. 2005 Aug 30;102(35):12383-8
pubmed: 16113084
J Comput Chem. 2004 Oct;25(13):1605-12
pubmed: 15264254
IEEE Trans Med Imaging. 1999 Aug;18(8):712-21
pubmed: 10534053
Phys Rev E Stat Nonlin Soft Matter Phys. 2009 Nov;80(5 Pt 1):051305
pubmed: 20364978
J Struct Biol. 2008 Mar;161(3):276-86
pubmed: 17720536
Biomech Model Mechanobiol. 2019 Aug;18(4):1177-1187
pubmed: 30843134
Nat Rev Mol Cell Biol. 2009 Jan;10(1):21-33
pubmed: 19197329
Nat Cell Biol. 2010 Sep;12(9):909-15
pubmed: 20694000
J Struct Biol. 2005 Oct;152(1):36-51
pubmed: 16182563
Nature. 1982 Jul 8;298(5870):131-5
pubmed: 7201078
Nat Methods. 2020 Feb;17(2):201-208
pubmed: 31768058
Nat Methods. 2007 Mar;4(3):215-7
pubmed: 17277781
EMBO J. 2011 Nov 16;30(22):4539-53
pubmed: 21926969
Structure. 2019 Aug 6;27(8):1211-1223.e5
pubmed: 31230946
J Struct Biol. 2005 Mar;149(3):227-34
pubmed: 15721576
Cells. 2019 Jan 16;8(1):
pubmed: 30654455
J Struct Biol. 2012 Dec;180(3):488-96
pubmed: 23000705
Nat Phys. 2019 May;15(5):509-516
pubmed: 31754369
Science. 2016 Feb 26;351(6276):969-72
pubmed: 26917770
Nat Methods. 2018 Nov;15(11):955-961
pubmed: 30349041
Nat Methods. 2017 Mar;14(3):290-296
pubmed: 28165473
Science. 2016 Jul 29;353(6298):506-8
pubmed: 27417497
Structure. 2015 Jan 6;23(1):173-182
pubmed: 25533486
Science. 1995 Apr 28;268(5210):533-9
pubmed: 7725097
J Mol Biol. 2012 Jun 22;419(5):359-68
pubmed: 22459261
Exp Cell Res. 2016 Apr 10;343(1):14-20
pubmed: 26519907
Nat Methods. 2012 Jan 08;9(2):185-8
pubmed: 22231642
J Struct Biol. 2017 Jun;198(3):163-176
pubmed: 28193500
Nature. 1990 Sep 6;347(6288):44-9
pubmed: 2395461
J Mol Biol. 2003 Oct 31;333(4):721-45
pubmed: 14568533
Nat Cell Biol. 2017 Jun;19(6):689-697
pubmed: 28530659
Nat Methods. 2017 Nov;14(11):1090-1096
pubmed: 28945706
Cell. 2003 Feb 21;112(4):453-65
pubmed: 12600310
J Struct Biol. 2019 May 1;206(2):149-155
pubmed: 30822456
Nat Protoc. 2016 Nov;11(11):2054-65
pubmed: 27685097
J Struct Biol. 2007 Nov;160(2):135-45
pubmed: 17884579
Nat Struct Mol Biol. 2018 Jun;25(6):528-537
pubmed: 29867215
Annu Rev Biochem. 2005;74:833-65
pubmed: 15952904
Nat Methods. 2013 Jun;10(6):584-90
pubmed: 23644547
Nature. 2010 Jul 8;466(7303):263-6
pubmed: 20613844
Cell. 1998 Apr 3;93(1):125-38
pubmed: 9546398
BMC Bioinformatics. 2016 Oct 5;17(1):405
pubmed: 27716029
Nat Cell Biol. 2007 Aug;9(8):858-67
pubmed: 17671451
Elife. 2020 Nov 13;9:
pubmed: 33185186
Nat Methods. 2016 Jan;13(1):24-7
pubmed: 27110629
Science. 2002 Nov 8;298(5596):1209-13
pubmed: 12424373
Nat Methods. 2019 Aug;16(8):757-762
pubmed: 31363205
Nature. 2010 Nov 25;468(7323):580-4
pubmed: 21107430
Nat Methods. 2019 Nov;16(11):1161-1168
pubmed: 31611690
Commun Integr Biol. 2011 May;4(3):344-5
pubmed: 21980576
Nat Methods. 2020 Feb;17(2):209-216
pubmed: 31907446
Proc Natl Acad Sci U S A. 2005 Mar 29;102(13):4729-34
pubmed: 15774580
Trends Cell Biol. 2016 Nov;26(11):825-837
pubmed: 27671779
Nat Methods. 2017 Oct;14(10):983-985
pubmed: 28846087
J Struct Biol. 2012 Dec;180(3):519-30
pubmed: 23000701