Healthy Cotwins Share Gut Microbiome Signatures With Their Inflammatory Bowel Disease Twins and Unrelated Patients.
Adult
Antigens, Bacterial
/ biosynthesis
Case-Control Studies
Cross-Sectional Studies
Feces
/ microbiology
Female
Gastrointestinal Microbiome
/ physiology
Humans
Inflammatory Breast Neoplasms
/ epidemiology
Male
Metagenomics
Middle Aged
Netherlands
/ epidemiology
Phenotype
Risk Factors
Siderophores
/ biosynthesis
Twins, Dizygotic
Twins, Monozygotic
Young Adult
Crohn’s Disease
Discordant Twin Design
Family Studies
Microbiota
Preclinical
Prediagnostic
Prediction
Ulcerative Colitis
Journal
Gastroenterology
ISSN: 1528-0012
Titre abrégé: Gastroenterology
Pays: United States
ID NLM: 0374630
Informations de publication
Date de publication:
05 2021
05 2021
Historique:
received:
07
08
2020
revised:
06
01
2021
accepted:
07
01
2021
pubmed:
22
1
2021
medline:
8
9
2021
entrez:
21
1
2021
Statut:
ppublish
Résumé
It is currently unclear whether reported changes in the gut microbiome are cause or consequence of inflammatory bowel disease (IBD). Therefore, we studied the gut microbiome of IBD-discordant and -concordant twin pairs, which offers the unique opportunity to assess individuals at increased risk of developing IBD, namely healthy cotwins from IBD-discordant twin pairs. Fecal samples were obtained from 99 twins (belonging to 51 twin pairs), 495 healthy age-, sex-, and body mass index-matched controls, and 99 unrelated patients with IBD. Whole-genome metagenomic shotgun sequencing was performed. Taxonomic and functional (pathways) composition was compared among healthy cotwins, IBD-twins, unrelated patients with IBD, and healthy controls with multivariable (ie, adjusted for potential confounding) generalized linear models. No significant differences were observed in the relative abundance of species and pathways between healthy cotwins and their IBD-twins (false discovery rate <0.10). Compared with healthy controls, 13, 19, and 18 species, and 78, 105, and 153 pathways were found to be differentially abundant in healthy cotwins, IBD-twins, and unrelated patients with IBD, respectively (false discovery rate <0.10). Of these, 8 (42.1%) of 19 and 1 (5.6%) of 18 species, and 37 (35.2%) of 105 and 30 (19.6%) of 153 pathways overlapped between healthy cotwins and IBD-twins, and healthy cotwins and unrelated patients with IBD, respectively. Many of the shared species and pathways have previously been associated with IBD. The shared pathways include potentially inflammation-related pathways, for example, an increase in propionate degradation and L-arginine degradation pathways. The gut microbiome of healthy cotwins from IBD-discordant twin pairs displays IBD-like signatures. These IBD-like microbiome signatures might precede the onset of IBD. However, longitudinal follow-up studies are needed to infer a causal relationship.
Sections du résumé
BACKGROUND & AIMS
It is currently unclear whether reported changes in the gut microbiome are cause or consequence of inflammatory bowel disease (IBD). Therefore, we studied the gut microbiome of IBD-discordant and -concordant twin pairs, which offers the unique opportunity to assess individuals at increased risk of developing IBD, namely healthy cotwins from IBD-discordant twin pairs.
METHODS
Fecal samples were obtained from 99 twins (belonging to 51 twin pairs), 495 healthy age-, sex-, and body mass index-matched controls, and 99 unrelated patients with IBD. Whole-genome metagenomic shotgun sequencing was performed. Taxonomic and functional (pathways) composition was compared among healthy cotwins, IBD-twins, unrelated patients with IBD, and healthy controls with multivariable (ie, adjusted for potential confounding) generalized linear models.
RESULTS
No significant differences were observed in the relative abundance of species and pathways between healthy cotwins and their IBD-twins (false discovery rate <0.10). Compared with healthy controls, 13, 19, and 18 species, and 78, 105, and 153 pathways were found to be differentially abundant in healthy cotwins, IBD-twins, and unrelated patients with IBD, respectively (false discovery rate <0.10). Of these, 8 (42.1%) of 19 and 1 (5.6%) of 18 species, and 37 (35.2%) of 105 and 30 (19.6%) of 153 pathways overlapped between healthy cotwins and IBD-twins, and healthy cotwins and unrelated patients with IBD, respectively. Many of the shared species and pathways have previously been associated with IBD. The shared pathways include potentially inflammation-related pathways, for example, an increase in propionate degradation and L-arginine degradation pathways.
CONCLUSIONS
The gut microbiome of healthy cotwins from IBD-discordant twin pairs displays IBD-like signatures. These IBD-like microbiome signatures might precede the onset of IBD. However, longitudinal follow-up studies are needed to infer a causal relationship.
Identifiants
pubmed: 33476671
pii: S0016-5085(21)00097-4
doi: 10.1053/j.gastro.2021.01.030
pii:
doi:
Substances chimiques
Antigens, Bacterial
0
Siderophores
0
enterobacterial common antigen
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Twin Study
Langues
eng
Sous-ensembles de citation
IM
Pagination
1970-1985Investigateurs
Bas Oldenburg
(B)
Femke van Wijk
(F)
Eelco C Brand
(EC)
Pieter Honkoop
(P)
Rutger J Jacobs
(RJ)
Cyriel Y Ponsioen
(CY)
Nanne K H de Boer
(NKH)
Yasser A Alderlieste
(YA)
Margot A van Herwaarden
(MA)
Sebastiaan A C van Tuyl
(SAC)
Maurice W Lutgens
(MW)
C Janneke van der Woude
(C)
Wout G M Mares
(WGM)
Daan B de Koning
(DB)
Joukje H Bosman
(JH)
Juda Vecht
(J)
Anneke M P de Schryver
(AMP)
Andrea E van der Meulen-de Jong
(AE)
Marieke J Pierik
(MJ)
Paul J Boekema
(PJ)
Robert J Verburg
(RJ)
Bindia Jharap
(B)
Gonneke Willemsen
(G)
Dorret I Boomsma
(DI)
Jeroen M Jansen
(JM)
Pieter C F Stokkers
(PCF)
Frank Hoentjen
(F)
Rutger Quispel
(R)
Carmen S Horjus Talabur Horje
(CS)
Paul C van de Meeberg
(PC)
Nofel Mahmmod
(N)
Rachel L West
(RL)
Marleen Willems
(M)
Itta M Minderhoud
(IM)
Herma H Fidder
(HH)
Fiona D M van Schaik
(FDM)
Meike M C Hirdes
(MMC)
Nynke A Boontje
(NA)
Bart L M Müskens
(BLM)
Rinse K Weersma
(RK)
Marielle J L Romberg-Camps
(MJL)
Informations de copyright
Copyright © 2021 The Authors. Published by Elsevier Inc. All rights reserved.