In Vitro Macrophage Immunomodulation by Poly(ε-caprolactone) Based-Coated AZ31 Mg Alloy.
Alloys
/ pharmacology
Animals
Biodegradable Plastics
/ chemistry
Coumarins
/ chemistry
Gene Expression Regulation
/ drug effects
Humans
Immunomodulation
/ drug effects
Inflammation
/ drug therapy
Macrophages
/ drug effects
Magnesium
/ chemistry
Metal Nanoparticles
/ chemistry
Mice
Osteogenesis
/ drug effects
Polyesters
/ pharmacology
RANK Ligand
/ genetics
RAW 264.7 Cells
Spectroscopy, Fourier Transform Infrared
Zinc Oxide
/ pharmacology
PCL-coating
electrochemical behaviour
inflammatory response
macrophage
magnesium alloy
osteoclastogenesis
Journal
International journal of molecular sciences
ISSN: 1422-0067
Titre abrégé: Int J Mol Sci
Pays: Switzerland
ID NLM: 101092791
Informations de publication
Date de publication:
18 Jan 2021
18 Jan 2021
Historique:
received:
30
11
2020
revised:
14
01
2021
accepted:
14
01
2021
entrez:
22
1
2021
pubmed:
23
1
2021
medline:
8
9
2021
Statut:
epublish
Résumé
Due to its excellent bone-like mechanical properties and non-toxicity, magnesium (Mg) and its alloys have attracted great interest as biomaterials for orthopaedic applications. However, their fast degradation rate in physiological environments leads to an acute inflammatory response, restricting their use as biodegradable metallic implants. Endowing Mg-based biomaterials with immunomodulatory properties can help trigger a desired immune response capable of supporting a favorable healing process. In this study, electrospun poly(ε-caprolactone) (PCL) fibers loaded with coumarin (CM) and/or zinc oxide nanoparticles (ZnO) were used to coat the commercial AZ31 Mg alloy as single and combined formulas, and their effects on the macrophage inflammatory response and osteoclastogenic process were investigated by indirect contact studies. Likewise, the capacity of the analyzed samples to generate reactive oxygen species (ROS) has been investigated. The data obtained by attenuated total reflection Fourier-transform infrared (FTIR-ATR) and X-ray photoelectron spectroscopy (XPS) analyses indicate that AZ31 alloy was perfectly coated with the PCL fibers loaded with CM and ZnO, which had an important influence on tuning the release of the active ingredient. Furthermore, in terms of degradation in phosphate-buffered saline (PBS) solution, the PCL-ZnO- and secondary PCL-CM-ZnO-coated samples exhibited the best corrosion behaviour. The in vitro results showed the PCL-CM-ZnO and, to a lower extent, PCL-ZnO coated sample exhibited the best behaviour in terms of inflammatory response and receptor activator of nuclear factor kappa-B ligand (RANKL)-mediated differentiation of RAW 264.7 macrophages into osteoclasts. Altogether, the results obtained suggest that the coating of Mg alloys with fibrous PCL containing CM and/or ZnO can constitute a feasible strategy for biomedical applications.
Identifiants
pubmed: 33477539
pii: ijms22020909
doi: 10.3390/ijms22020909
pmc: PMC7831122
pii:
doi:
Substances chimiques
Alloys
0
Biodegradable Plastics
0
Coumarins
0
Mg-Al-Zn-Mn-Si-Cu alloy
0
Polyesters
0
RANK Ligand
0
Tnfsf11 protein, mouse
0
polycaprolactone
24980-41-4
coumarin
A4VZ22K1WT
Magnesium
I38ZP9992A
Zinc Oxide
SOI2LOH54Z
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Romanian Ministry of National Education, UEFISCDI
ID : Grant 65PCCDI/ 2018
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