T-Cell Lymphoma Clonality by Copy Number Variation Analysis of T-Cell Receptor Genes.

T-cell lymphoma T-cell receptor clonality copy number variation analysis whole genome sequencing

Journal

Cancers
ISSN: 2072-6694
Titre abrégé: Cancers (Basel)
Pays: Switzerland
ID NLM: 101526829

Informations de publication

Date de publication:
19 Jan 2021
Historique:
received: 25 12 2020
accepted: 11 01 2021
entrez: 22 1 2021
pubmed: 23 1 2021
medline: 23 1 2021
Statut: epublish

Résumé

T-cell lymphomas arise from a single neoplastic clone and exhibit identical patterns of deletions in T-cell receptor (TCR) genes. Whole genome sequencing (WGS) data represent a treasure trove of information for the development of novel clinical applications. However, the use of WGS to identify clonal T-cell proliferations has not been systematically studied. In this study, based on WGS data, we identified monoclonal rearrangements (MRs) of T-cell receptors (TCR) genes using a novel segmentation algorithm and copy number computation. We evaluated the feasibility of this technique as a marker of T-cell clonality using T-cell lymphomas (TCL,

Identifiants

pubmed: 33477749
pii: cancers13020340
doi: 10.3390/cancers13020340
pmc: PMC7832336
pii:
doi:

Types de publication

Journal Article

Langues

eng

Subventions

Organisme : National Medical Research Council
ID : CSAINV17nov016, WBS R-179-000-063-213
Organisme : National Medical Research Council
ID : SYMPHONY)(NMRC May 2018 / OF-LCG
Organisme : National Medical Research Council
ID : NMRC-OFIRG16NOV090

Références

Virchows Arch. 2020 Dec;477(6):873-883
pubmed: 32314054
Leukemia. 2019 Sep;33(9):2241-2253
pubmed: 31243313
Immunology. 2011 Sep;134(1):17-32
pubmed: 21711350
J Mol Diagn. 2010 Nov;12(6):787-96
pubmed: 20959612
J Mol Diagn. 2008 Nov;10(6):502-12
pubmed: 18832464
Leukemia. 2003 Dec;17(12):2257-317
pubmed: 14671650
J Clin Pathol. 2003 Jan;56(1):1-11
pubmed: 12499424
J Mol Diagn. 2002 May;4(2):81-9
pubmed: 11986398
Leukemia. 2006 Jan;20(1):176-9
pubmed: 16307010
Sci Transl Med. 2018 May 9;10(440):
pubmed: 29743350
J Clin Pathol. 2018 Mar;71(3):195-200
pubmed: 29055897
Sci Transl Med. 2015 Oct 7;7(308):308ra158
pubmed: 26446955
J Mol Diagn. 2006 Sep;8(4):466-75; quiz 527
pubmed: 16931587
Leukemia. 2007 Feb;21(2):215-21
pubmed: 17170730
Leukemia. 2019 Sep;33(9):2227-2240
pubmed: 31197258
BMC Genomics. 2012 Nov 04;13:591
pubmed: 23442169
J Immunol. 1995 Apr 1;154(7):3538-47
pubmed: 7897233
Int Immunol. 2005 Mar;17(3):225-32
pubmed: 15642954
Leukemia. 2012 Oct;26(10):2159-71
pubmed: 22918122
Nat Methods. 2015 May;12(5):380-1
pubmed: 25924071
Haematologica. 2018 Feb;103(2):278-287
pubmed: 29097495
Autoimmunity. 2016;49(3):147-54
pubmed: 26829107
Genet Med. 2018 Oct;20(10):1196-1205
pubmed: 29388947
BMC Genomics. 2014 May 28;15:409
pubmed: 24885090
PLoS Comput Biol. 2016 Apr 21;12(4):e1004873
pubmed: 27100738
J Hematop. 2008 Sep;1(2):97-109
pubmed: 19669208
Hum Immunol. 1996 Jun-Jul;48(1-2):77-83
pubmed: 8824576
Blood Cells Mol Dis. 2003 Jan-Feb;30(1):1-12
pubmed: 12667982
Am J Clin Pathol. 2013 Feb;139(2):231-41
pubmed: 23355208

Auteurs

Ming Liang Oon (ML)

Department of Pathology, National University Hospital, National University Health System, Singapore 119074, Singapore.

Jing Quan Lim (JQ)

Lymphoma Genomic Translational Research Laboratory, Division of Cellular and Molecular Research, National Cancer Centre Singapore, Singapore 169610, Singapore.
Cancer and Stem Cell Biology, Duke-NUS Medical School, Singapore 169857, Singapore.

Bernett Lee (B)

Singapore Immunology Network (SIgN), A*STAR (Agency for Science, Technology and Research), Singapore 138632, Singapore.

Sai Mun Leong (SM)

Department of Pathology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore 119074, Singapore.

Gwyneth Shook-Ting Soon (GS)

Department of Pathology, National University Hospital, National University Health System, Singapore 119074, Singapore.

Zi Wei Wong (ZW)

Department of Pathology, National University Hospital, National University Health System, Singapore 119074, Singapore.

Evelyn Huizi Lim (EH)

Viva-NUS Centre for Translational Research in Acute Leukaemia, Department of Paediatrics, Yong Loo Lin School of Medicine, National University of Singapore, Singapore 117597, Singapore.

Zhenhua Li (Z)

Viva-NUS Centre for Translational Research in Acute Leukaemia, Department of Paediatrics, Yong Loo Lin School of Medicine, National University of Singapore, Singapore 117597, Singapore.

Allen Eng Juh Yeoh (AEJ)

Viva-NUS Centre for Translational Research in Acute Leukaemia, Department of Paediatrics, Yong Loo Lin School of Medicine, National University of Singapore, Singapore 117597, Singapore.
VIVA-University Children's Cancer Centre, Khoo Teck Puat-National University Children's Medical Institute, National University Hospital, National University Health System, Singapore 119074, Singapore.

Shangying Chen (S)

Department of Biochemistry, Yong Loo Lin School of Medicine, National University of Singapore, Singapore 117596, Singapore.

Kenneth Hon Kim Ban (KHK)

Department of Biochemistry, Yong Loo Lin School of Medicine, National University of Singapore, Singapore 117596, Singapore.

Tae-Hoon Chung (TH)

Cancer Science Institute of Singapore, National University of Singapore, Singapore 117599, Singapore.

Soo-Yong Tan (SY)

Department of Pathology, National University Hospital, National University Health System, Singapore 119074, Singapore.
Department of Pathology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore 119074, Singapore.

Shih-Sung Chuang (SS)

Department of Pathology, Chi-Mei Medical Center, Tainan 71004, Taiwan.

Seiichi Kato (S)

Department of Pathology and Laboratory Medicine, Nagoya University Hospital, Nagoya 466-8560, Japan.
Department of Pathology and Molecular Diagnostics, Aichi Cancer Center Hospital, Nagoya 464-0021, Japan.

Shigeo Nakamura (S)

Department of Pathology and Laboratory Medicine, Nagoya University Hospital, Nagoya 466-8560, Japan.

Emiko Takahashi (E)

Department of Pathology, Aichi Medical University Hospital, Nagakute 480-1195, Japan.

Yong-Howe Ho (YH)

Department of Pathology, Tan Tock Seng Hospital, Singapore 308433, Singapore.

Joseph D Khoury (JD)

Department of Hematopathology, The University of Texas MD Anderson Cancer Center, Houston, TX 77030, USA.

Rex K H Au-Yeung (RKH)

Department of Pathology, Queen Mary Hospital, The University of Hong Kong, Hong Kong, China.

Chee-Leong Cheng (CL)

Department of Pathology, Singapore General Hospital, Singapore 169608, Singapore.

Soon-Thye Lim (ST)

Lymphoma Genomic Translational Research Laboratory, Division of Medical Oncology, National Cancer Centre Singapore, Singapore 169610, Singapore.

Wee-Joo Chng (WJ)

Cancer Science Institute of Singapore, National University of Singapore, Singapore 117599, Singapore.
Department of Hematology-Oncology, National University Cancer Institute Singapore, National University Hospital, National University Health System, Singapore 119074, Singapore.

Claudio Tripodo (C)

Tumor Immunology Unit, University of Palermo School of Medicine, 90134 Palermo, Italy.

Olaf Rotzschke (O)

Singapore Immunology Network (SIgN), A*STAR (Agency for Science, Technology and Research), Singapore 138632, Singapore.

Choon Kiat Ong (CK)

Lymphoma Genomic Translational Research Laboratory, Division of Cellular and Molecular Research, National Cancer Centre Singapore, Singapore 169610, Singapore.
Cancer and Stem Cell Biology, Duke-NUS Medical School, Singapore 169857, Singapore.
Genome Institute of Singapore, A*STAR (Agency for Science, Technology and Research), Singapore 138632, Singapore.

Siok-Bian Ng (SB)

Department of Pathology, National University Hospital, National University Health System, Singapore 119074, Singapore.
Department of Pathology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore 119074, Singapore.
Cancer Science Institute of Singapore, National University of Singapore, Singapore 117599, Singapore.

Classifications MeSH