Steroidal Regulation of Oviductal microRNAs Is Associated with microRNA-Processing in Beef Cows.
Animals
Cattle
/ genetics
Estrous Cycle
/ drug effects
Fallopian Tubes
/ drug effects
Female
Gene Expression Profiling
/ veterinary
Gene Expression Regulation
/ drug effects
Gonadal Steroid Hormones
/ pharmacology
MicroRNAs
/ drug effects
Ovarian Follicle
/ drug effects
Ovulation
/ drug effects
RNA Processing, Post-Transcriptional
/ drug effects
Transcriptome
/ drug effects
ampulla
cattle
estradiol
isthmus
progesterone
Journal
International journal of molecular sciences
ISSN: 1422-0067
Titre abrégé: Int J Mol Sci
Pays: Switzerland
ID NLM: 101092791
Informations de publication
Date de publication:
19 Jan 2021
19 Jan 2021
Historique:
received:
15
12
2020
revised:
12
01
2021
accepted:
13
01
2021
entrez:
22
1
2021
pubmed:
23
1
2021
medline:
7
9
2021
Statut:
epublish
Résumé
Information on molecular mechanisms through which sex-steroids regulate oviductal function to support early embryo development is lacking. Here, we hypothesized that the periovulatory endocrine milieu affects the miRNA processing machinery and miRNA expression in bovine oviductal tissues. Growth of the preovulatory follicle was controlled to obtain cows that ovulated a small follicle (SF) and subsequently bore a small corpus luteum (CL; SF-SCL) or a large follicle (LF) and large CL (LF-LCL). These groups differed in the periovulatory plasmatic sex-steroid's concentrations. Ampulla and isthmus samples were collected on day four of the estrous cycle. Abundance of DROSHA, DICER1, and AGO4 transcripts was greater in the ampulla than the isthmus. In the ampulla, transcription of these genes was greater for the SF-SCL group, while the opposite was observed in the isthmus. The expression of the 88 most abundant miRNAs and 14 miRNAs in the ampulla and 34 miRNAs in isthmus were differentially expressed between LF-LCL and SF-SCL groups. Integration of transcriptomic and miRNA data and molecular pathways enrichment showed that important pathways were inhibited in the SF-SCL group due to miRNA control. In conclusion, the endocrine milieu affects the miRNA expression in the bovine oviduct in a region-specific manner.
Identifiants
pubmed: 33477993
pii: ijms22020953
doi: 10.3390/ijms22020953
pmc: PMC7835783
pii:
doi:
Substances chimiques
Gonadal Steroid Hormones
0
MicroRNAs
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
ID : 15068-12-9
Organisme : Conselho Nacional de Desenvolvimento Científico e Tecnológico
ID : N/A
Organisme : Fundação de Amparo à Pesquisa do Estado de São Paulo
ID : 2011/03226-4
Références
Mol Cell. 2004 Jul 23;15(2):185-97
pubmed: 15260970
Bioessays. 1994 Jan;16(1):31-8
pubmed: 8141805
Mol Endocrinol. 2008 Oct;22(10):2336-52
pubmed: 18687735
World J Biol Chem. 2017 Feb 26;8(1):45-56
pubmed: 28289518
Cell. 2018 Mar 22;173(1):20-51
pubmed: 29570994
Genome Biol. 2004;5(10):R80
pubmed: 15461798
Cell Cycle. 2008 Oct;7(20):3143-8
pubmed: 18843198
Proc Natl Acad Sci U S A. 2009 Sep 15;106(37):15732-7
pubmed: 19706389
Biol Reprod. 2015 Aug;93(2):52
pubmed: 26178716
PLoS One. 2015 Dec 23;10(12):e0145321
pubmed: 26699362
Bioinformatics. 2009 Aug 15;25(16):2078-9
pubmed: 19505943
Endocrine. 2010 Apr;37(2):265-73
pubmed: 20852728
Semin Cell Dev Biol. 2017 May;65:20-28
pubmed: 27235676
Biol Reprod. 1995 Dec;53(6):1527-43
pubmed: 8562711
Cell. 2009 Feb 20;136(4):642-55
pubmed: 19239886
Genom Data. 2017 Jun 15;13:27-29
pubmed: 28664146
Theriogenology. 2020 Jan 15;142:131-137
pubmed: 31593880
Mol Cell Endocrinol. 2012 Jun 24;357(1-2):50-9
pubmed: 21952083
Cell Tissue Res. 2017 Nov;370(2):319-333
pubmed: 28770380
Sci Rep. 2018 Oct 31;8(1):16094
pubmed: 30382141
BMC Bioinformatics. 2018 Feb 20;19(1):56
pubmed: 29458351
Dev Cell. 2013 Jun 24;25(6):553-4
pubmed: 23806615
OMICS. 2012 May;16(5):284-7
pubmed: 22455463
Trends Endocrinol Metab. 2012 May;23(5):223-33
pubmed: 22503553
RNA. 2014 May;20(5):621-31
pubmed: 24677349
Reprod Biol Endocrinol. 2015 May 10;13:39
pubmed: 25957795
Biochim Biophys Acta. 2011 Nov-Dec;1809(11-12):700-7
pubmed: 21683814
Theriogenology. 2016 Apr 15;85(7):1239-48
pubmed: 26764150
Nucleic Acids Res. 2009 Aug;37(14):4850-61
pubmed: 19528081
Biotech Histochem. 2011 Aug;86(4):262-71
pubmed: 20438296
Horm Cancer. 2010 Dec;1(6):306-19
pubmed: 21761362
J Biochem. 2010 Oct;148(4):381-92
pubmed: 20833630
Genome Biol. 2010;11(10):R106
pubmed: 20979621
J Anim Sci. 2017 Nov;95(11):4857-4868
pubmed: 29293730
Nat Methods. 2012 Mar 04;9(4):357-9
pubmed: 22388286
Reprod Biol Endocrinol. 2015 Aug 05;13:84
pubmed: 26242217
Biol Reprod. 2000 Jun;62(6):1745-53
pubmed: 10819779
BMC Genomics. 2018 Aug 22;19(1):622
pubmed: 30134841
Theriogenology. 2014 Apr 1;81(6):861-9
pubmed: 24507960
Hum Reprod. 2019 Oct 2;34(10):1984-1998
pubmed: 31625574
PLoS One. 2017 Jun 15;12(6):e0179451
pubmed: 28617821
Genome Biol. 2009;10(9):R90
pubmed: 19723326
Genome Biol. 2013 Apr 25;14(4):R36
pubmed: 23618408
Biology (Basel). 2018 Feb 02;7(1):
pubmed: 29393864
Biol Reprod. 2018 Sep 1;99(3):590-599
pubmed: 29659700
Physiol Genomics. 2010 Nov 29;42A(4):290-300
pubmed: 20876846
Reprod Fertil Dev. 2014 Apr 21;:
pubmed: 25896641