Minimal genome-wide human CRISPR-Cas9 library.
CRISPR-Cas9
Genome-wide
KS score
Minimal library
Organoid
Journal
Genome biology
ISSN: 1474-760X
Titre abrégé: Genome Biol
Pays: England
ID NLM: 100960660
Informations de publication
Date de publication:
21 01 2021
21 01 2021
Historique:
received:
08
05
2020
accepted:
07
01
2021
entrez:
22
1
2021
pubmed:
23
1
2021
medline:
15
12
2021
Statut:
epublish
Résumé
CRISPR guide RNA libraries have been iteratively improved to provide increasingly efficient reagents, although their large size is a barrier for many applications. We design an optimised minimal genome-wide human CRISPR-Cas9 library (MinLibCas9) by mining existing large-scale gene loss-of-function datasets, resulting in a greater than 42% reduction in size compared to other CRISPR-Cas9 libraries while preserving assay sensitivity and specificity. MinLibCas9 provides backward compatibility with existing datasets, increases the dynamic range of CRISPR-Cas9 screens and extends their application to complex models and assays.
Identifiants
pubmed: 33478580
doi: 10.1186/s13059-021-02268-4
pii: 10.1186/s13059-021-02268-4
pmc: PMC7818936
doi:
Substances chimiques
RNA, Guide
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
40Subventions
Organisme : Wellcome Trust
ID : 206194
Pays : United Kingdom
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