Bovine NK-lysin-derived peptides have bactericidal effects against Mycobacterium avium subspecies paratuberculosis.
Amps
Antimicrobial peptides
Bovine NK-lysins
Johne’s disease
MAP
Mycobacterium avium subspecies paratuberculosis
Propidium monoazide-based viability qPCR
bNK2A
Journal
Veterinary research
ISSN: 1297-9716
Titre abrégé: Vet Res
Pays: England
ID NLM: 9309551
Informations de publication
Date de publication:
21 Jan 2021
21 Jan 2021
Historique:
received:
26
10
2020
accepted:
04
01
2021
entrez:
22
1
2021
pubmed:
23
1
2021
medline:
3
8
2021
Statut:
epublish
Résumé
Infection with Mycobacterium avium subspecies paratuberculosis (MAP) is complex, but little is known about the role that natural killer (NK) cells play. In the present study, four bovine NK-lysin peptides were synthesized to evaluate their bactericidal activity against MAP. The results demonstrated that bNK-lysin peptides were directly bactericidal against MAP, with bNK1 and bNK2A being more potent than bNK2B and bNK2C. Mechanistically, transmission electron microscopy revealed that the incubation of MAP with bNK2A resulted in extensive damage to cell membranes and cytosolic content leakage. Furthermore, the addition of bNK2A linked with a cell-penetrating peptide resulted in increased MAP killing in a macrophage model.
Identifiants
pubmed: 33478585
doi: 10.1186/s13567-021-00893-2
pii: 10.1186/s13567-021-00893-2
pmc: PMC7818946
doi:
Substances chimiques
Anti-Bacterial Agents
0
NK-lysin
0
Proteolipids
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
11Subventions
Organisme : Agricultural Research Service
ID : 5030-32000-116-00-D
Organisme : Agricultural Research Service
ID : 5030-32000-221-00-D
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