Isolation of Borrelia miyamotoi and other Borreliae using a modified BSK medium.
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
21 01 2021
21 01 2021
Historique:
received:
03
06
2020
accepted:
16
12
2020
entrez:
22
1
2021
pubmed:
23
1
2021
medline:
24
9
2021
Statut:
epublish
Résumé
Borrelia spirochetes are the causative agents of Lyme borreliosis (LB) and relapsing fever (RF). Despite the steady rise in infections and the identification of new species causing human illness over the last decade, isolation of borreliae in culture has become increasingly rare. A modified Barbour-Stoenner-Kelly (BSK) media formulation, BSK-R, was developed for isolation of the emerging RF pathogen, Borrelia miyamotoi. BSK-R is a diluted BSK-II derivative supplemented with Lebovitz's L-15, mouse and fetal calf serum. Decreasing the concentration of CMRL 1066 and other components was essential for growth of North American B. miyamotoi. Sixteen B. miyamotoi isolates, originating from Ixodes scapularis ticks, rodent and human blood collected in the eastern and upper midwestern United States, were isolated and propagated to densities > 10
Identifiants
pubmed: 33479281
doi: 10.1038/s41598-021-81252-1
pii: 10.1038/s41598-021-81252-1
pmc: PMC7820315
doi:
Substances chimiques
Culture Media
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1926Références
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