Methods to Study the Transcriptome of Regulatory B Cells.
B Cells
Gene expression profiling
Microarrays
RT-qPCR
mRNA
Journal
Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969
Informations de publication
Date de publication:
2021
2021
Historique:
entrez:
22
1
2021
pubmed:
23
1
2021
medline:
7
4
2021
Statut:
ppublish
Résumé
Regulatory B cells do not constitute a distinct cell lineage because no unique marker or set of markers can exclusively identify neither murine nor human regulatory B cells, and efficient IL-10 production is their only known distinguishing feature. After purification of IL-10-secreting B cells, one may want to characterize them by analyzing their gene expression profile. This goal can be achieved by using different technologies: RT-qPCR, microarrays, Nanostring's nCounter technology, Biomark HD are techniques that will allow you to analyze their gene expression, whether in a targeted (RT-qPCR), extended but targeted (Nanostring's nCounter technology, Biomark HD) or exhaustive (Microarray) way. Aim of this chapter is the description of these techniques in the view of their application to the study and characterization of regulatory B cells.
Identifiants
pubmed: 33479905
doi: 10.1007/978-1-0716-1237-8_16
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
295-305Références
Chattopadhyay PK, Gierahn TM, Roederer M, Love JC (2014) Single-cell technologies for monitoring immune systems. Nat Immunol 15(2):128–135. https://doi.org/10.1038/ni.2796
doi: 10.1038/ni.2796
pubmed: 24448570
pmcid: 4040085
Rincon-Arevalo H, Sanchez-Parra CC, Castano D, Yassin L, Vasquez G (2016) Regulatory B cells and mechanisms. Int Rev Immunol 35(2):156–176. https://doi.org/10.3109/08830185.2015.1015719
doi: 10.3109/08830185.2015.1015719
pubmed: 25793964
Rosser EC, Mauri C (2015) Regulatory B cells: origin, phenotype, and function. Immunity 42(4):607–612. https://doi.org/10.1016/j.immuni.2015.04.005
doi: 10.1016/j.immuni.2015.04.005
pubmed: 25902480
Zheng GX, Terry JM, Belgrader P, Ryvkin P, Bent ZW, Wilson R, Ziraldo SB, Wheeler TD, McDermott GP, Zhu J, Gregory MT, Shuga J, Montesclaros L, Underwood JG, Masquelier DA, Nishimura SY, Schnall-Levin M, Wyatt PW, Hindson CM, Bharadwaj R, Wong A, Ness KD, Beppu LW, Deeg HJ, McFarland C, Loeb KR, Valente WJ, Ericson NG, Stevens EA, Radich JP, Mikkelsen TS, Hindson BJ, Bielas JH (2017) Massively parallel digital transcriptional profiling of single cells. Nat Commun 8:14049. https://doi.org/10.1038/ncomms14049
doi: 10.1038/ncomms14049
pubmed: 28091601
pmcid: 5241818
Shalek AK, Satija R, Adiconis X, Gertner RS, Gaublomme JT, Raychowdhury R, Schwartz S, Yosef N, Malboeuf C, Lu D, Trombetta JJ, Gennert D, Gnirke A, Goren A, Hacohen N, Levin JZ, Park H, Regev A (2013) Single-cell transcriptomics reveals bimodality in expression and splicing in immune cells. Nature 498(7453):236–240. https://doi.org/10.1038/nature12172
doi: 10.1038/nature12172
pubmed: 23685454
pmcid: 3683364
Berkani N, Joly P, Golinski ML, Colliou N, Lim A, Larbi A, Riou G, Caillot F, Bernard P, Bedane C, Delaporte E, Chaby G, Dompmartin A, Hertl M, Calbo S, Musette P (2019) B-cell depletion induces a shift in self antigen specific B-cell repertoire and cytokine pattern in patients with bullous pemphigoid. Sci Rep 9(1):3525. https://doi.org/10.1038/s41598-019-40203-7
doi: 10.1038/s41598-019-40203-7
pubmed: 30837635
pmcid: 6401188
Hebert V, Petit M, Maho-Vaillant M, Golinski ML, Riou G, Derambure C, Boyer O, Joly P, Calbo S (2019) Modifications of the Transcriptomic profile of autoreactive B cells from pemphigus patients after treatment with rituximab or a standard corticosteroid regimen. Front Immunol 10:1794. https://doi.org/10.3389/fimmu.2019.01794
doi: 10.3389/fimmu.2019.01794
pubmed: 31440235
pmcid: 6693356
Golinski ML, Demeules M, Derambure C, Riou G, Maho-Vaillant M, Boyer O, Joly P, Calbo S (2020) CD11c(+) B cells are mainly memory cells, precursors of antibody secreting cells in healthy donors. Front Immunol 11:32. https://doi.org/10.3389/fimmu.2020.00032
doi: 10.3389/fimmu.2020.00032
pubmed: 32158442
pmcid: 7051942
Lin W, Cerny D, Chua E, Duan K, Yi JT, Shadan NB, Lum J, Maho-Vaillant M, Zolezzi F, Wong SC, Larbi A, Fink K, Musette P, Poidinger M, Calbo S (2014) Human regulatory B cells combine phenotypic and genetic hallmarks with a distinct differentiation fate. J Immunol 193(5):2258–2266. https://doi.org/10.4049/jimmunol.1303214
doi: 10.4049/jimmunol.1303214
pubmed: 25080484