Adult glut3 homozygous null mice survive to demonstrate neural excitability and altered neurobehavioral responses reminiscent of neurodevelopmental disorders.


Journal

Experimental neurology
ISSN: 1090-2430
Titre abrégé: Exp Neurol
Pays: United States
ID NLM: 0370712

Informations de publication

Date de publication:
04 2021
Historique:
received: 10 09 2020
revised: 05 01 2021
accepted: 09 01 2021
pubmed: 23 1 2021
medline: 18 9 2021
entrez: 22 1 2021
Statut: ppublish

Résumé

Since GLUT3 is vital for fueling neurotransmission, we examined in-vivo the adult phenotype carrying the conditional homozygous glut3 gene mutation (KO) in glutamate-excitatory neurons. These KO mice demonstrated sex-specific differences in brain and body weights (p = 0.0001 and p = 0.01 each) with reduced GLUT3 protein in cerebral cortices and brain stem (p = 0.005). In patch clamp studies the glut3 KO mice displayed a shorter latency to and enhanced paroxysmal activity (p = 0.01 and p = 0.015 each) in pyramidal neurons upon application of a GABA

Identifiants

pubmed: 33482226
pii: S0014-4886(21)00008-X
doi: 10.1016/j.expneurol.2021.113603
pmc: PMC8281499
mid: NIHMS1664458
pii:
doi:

Substances chimiques

Glucose Transporter Type 3 0

Types de publication

Journal Article Research Support, N.I.H., Extramural

Langues

eng

Sous-ensembles de citation

IM

Pagination

113603

Subventions

Organisme : NICHD NIH HHS
ID : R01 HD041230
Pays : United States
Organisme : NICHD NIH HHS
ID : R01 HD081206
Pays : United States

Informations de copyright

Copyright © 2021 Elsevier Inc. All rights reserved.

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Auteurs

Bo-Chul Shin (BC)

Departments of Pediatrics, Division of Neonatology & Developmental Biology, The Neonatal Research Center of the UCLA Children's Discovery & Innovation Institute, David Geffen School of Medicine at UCLA, Los Angeles, CA 90095-1752, United States of America.

Carlos Cepeda (C)

Intellectual and Developmental Disabilities Research Center and Brain Research Institute, David Geffen School of Medicine at UCLA, Los Angeles, CA 90095-1752, United States of America.

Mason Eghbali (M)

Departments of Pediatrics, Division of Neonatology & Developmental Biology, The Neonatal Research Center of the UCLA Children's Discovery & Innovation Institute, David Geffen School of Medicine at UCLA, Los Angeles, CA 90095-1752, United States of America.

Shin Yun Byun (SY)

Departments of Pediatrics, Division of Neonatology & Developmental Biology, The Neonatal Research Center of the UCLA Children's Discovery & Innovation Institute, David Geffen School of Medicine at UCLA, Los Angeles, CA 90095-1752, United States of America.

Michael S Levine (MS)

Intellectual and Developmental Disabilities Research Center and Brain Research Institute, David Geffen School of Medicine at UCLA, Los Angeles, CA 90095-1752, United States of America.

Sherin U Devaskar (SU)

Departments of Pediatrics, Division of Neonatology & Developmental Biology, The Neonatal Research Center of the UCLA Children's Discovery & Innovation Institute, David Geffen School of Medicine at UCLA, Los Angeles, CA 90095-1752, United States of America. Electronic address: Sdevaskar@mednet.ucla.edu.

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