A 3-Layered Bioartificial Blood Vessel with Physiological Wall Architecture Generated by Mechanical Stimulation.
Bioreactor technique
Fibrin matrix
Flow conditioning
Perfusion system
Pulsatile perfusion
Vascular graft
Vascular tissue engineering
Journal
Annals of biomedical engineering
ISSN: 1573-9686
Titre abrégé: Ann Biomed Eng
Pays: United States
ID NLM: 0361512
Informations de publication
Date de publication:
Sep 2021
Sep 2021
Historique:
received:
02
11
2020
accepted:
06
01
2021
pubmed:
24
1
2021
medline:
21
1
2022
entrez:
23
1
2021
Statut:
ppublish
Résumé
The generation of cellularized bioartificial blood vessels resembling all three layers of the natural vessel wall with physiological morphology and cell alignment is a long pursued goal in vascular tissue engineering. Simultaneous culture of all three layers under physiological mechanical conditions requires highly sophisticated perfusion techniques and still today remains a key challenge. Here, three-layered bioartificial vessels based on fibrin matrices were generated using a stepwise molding technique. Adipose-derived stem cells (ASC) were differentiated to smooth muscle cells (SMC) and integrated in a compacted tubular fibrin matrix to resemble the tunica media. The tunica adventitia-equivalent containing human umbilical vein endothelial cells (HUVEC) and ASC in a low concentration fibrin matrix was molded around it. Luminal seeding with HUVEC resembled the tunica intima. Subsequently, constructs were exposed to physiological mechanical stimulation in a pulsatile bioreactor for 72 h. Compared to statically incubated controls, mechanical stimulation induced physiological cell alignment in each layer: Luminal endothelial cells showed longitudinal alignment, cells in the media-layer were aligned circumferentially and expressed characteristic SMC marker proteins. HUVEC in the adventitia-layer formed longitudinally aligned microvascular tubes resembling vasa vasorum capillaries. Thus, physiologically organized three-layered bioartificial vessels were successfully manufactured by stepwise fibrin molding with subsequent mechanical stimulation.
Identifiants
pubmed: 33483842
doi: 10.1007/s10439-021-02728-9
pii: 10.1007/s10439-021-02728-9
doi:
Substances chimiques
Biocompatible Materials
0
Fibrin
9001-31-4
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
2066-2079Subventions
Organisme : German Society for Implant Research and Development
ID : Vascularization of bioartificial implants 2017-2020
Informations de copyright
© 2021. Biomedical Engineering Society.
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