SARS-CoV-2 seroconversion among 4040 Egyptian healthcare workers in 12 resource-limited healthcare facilities: A prospective cohort study.


Journal

International journal of infectious diseases : IJID : official publication of the International Society for Infectious Diseases
ISSN: 1878-3511
Titre abrégé: Int J Infect Dis
Pays: Canada
ID NLM: 9610933

Informations de publication

Date de publication:
Mar 2021
Historique:
received: 09 12 2020
revised: 31 12 2020
accepted: 10 01 2021
pubmed: 24 1 2021
medline: 24 4 2021
entrez: 23 1 2021
Statut: ppublish

Résumé

We examined Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) seroconversion incidence and risk factors 21 days after baseline screening among healthcare workers (HCWs) in a resource-limited setting. A prospective cohort study of 4040 HCWs took place at 12 university healthcare facilities in Cairo, Egypt; April-June 2020. Follow-up exposure and clinical data were collected through online survey. SARS-CoV-2 testing was done using rapid IgM and IgG serological tests and reverse transcriptase-polymerase chain reaction (RT-PCR) for those with positive serology. Cox proportional hazards modelling was used to estimate adjusted hazard ratios (HR) of seroconversion. 3870/4040 (95.8%) HCWs tested negative for IgM, IgG and PCR at baseline; 2282 (59.0%) returned for 21-day follow-up. Seroconversion incidence (positive IgM and/or IgG) was 100/2282 (4.4%, 95% CI:3.6-5.3), majority asymptomatic (64.0%); daily hazard of 0.21% (95% CI:0.17-0.25)/48 746 person-days of follow-up. Seroconversion was: 4.0% (64/1596; 95% CI:3.1-5.1) among asymptomatic; 5.3% (36/686; 95% CI:3.7-7.2) among symptomatic HCWs. Seroconversion was independently associated with older age; lower education; contact with a confirmed case >15 min; chronic kidney disease; pregnancy; change/loss of smell; and negatively associated with workplace contact. Most seroconversions were asymptomatic, emphasizing need for regular universal testing. Seropositivity was three-fold that observed at baseline. Cumulative infections increased nationally by a similar rate, suggesting HCW infections reflect community not nosocomial transmission.

Sections du résumé

BACKGROUND BACKGROUND
We examined Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) seroconversion incidence and risk factors 21 days after baseline screening among healthcare workers (HCWs) in a resource-limited setting.
METHODS METHODS
A prospective cohort study of 4040 HCWs took place at 12 university healthcare facilities in Cairo, Egypt; April-June 2020. Follow-up exposure and clinical data were collected through online survey. SARS-CoV-2 testing was done using rapid IgM and IgG serological tests and reverse transcriptase-polymerase chain reaction (RT-PCR) for those with positive serology. Cox proportional hazards modelling was used to estimate adjusted hazard ratios (HR) of seroconversion.
RESULTS RESULTS
3870/4040 (95.8%) HCWs tested negative for IgM, IgG and PCR at baseline; 2282 (59.0%) returned for 21-day follow-up. Seroconversion incidence (positive IgM and/or IgG) was 100/2282 (4.4%, 95% CI:3.6-5.3), majority asymptomatic (64.0%); daily hazard of 0.21% (95% CI:0.17-0.25)/48 746 person-days of follow-up. Seroconversion was: 4.0% (64/1596; 95% CI:3.1-5.1) among asymptomatic; 5.3% (36/686; 95% CI:3.7-7.2) among symptomatic HCWs. Seroconversion was independently associated with older age; lower education; contact with a confirmed case >15 min; chronic kidney disease; pregnancy; change/loss of smell; and negatively associated with workplace contact.
CONCLUSIONS CONCLUSIONS
Most seroconversions were asymptomatic, emphasizing need for regular universal testing. Seropositivity was three-fold that observed at baseline. Cumulative infections increased nationally by a similar rate, suggesting HCW infections reflect community not nosocomial transmission.

Identifiants

pubmed: 33484863
pii: S1201-9712(21)00048-5
doi: 10.1016/j.ijid.2021.01.037
pmc: PMC7817419
pii:
doi:

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

534-542

Informations de copyright

Copyright © 2021 The Author(s). Published by Elsevier Ltd.. All rights reserved.

Références

Lancet Infect Dis. 2020 Sep;20(9):1051-1060
pubmed: 32539986
Nat Med. 2020 Jul;26(7):1033-1036
pubmed: 32398876
Am J Epidemiol. 2021 Jan 4;190(1):161-175
pubmed: 32870978
J Clin Microbiol. 2020 Jul 23;58(8):
pubmed: 32381641
JAMA Intern Med. 2020 Oct 1;180(10):1356-1362
pubmed: 32808970
Pathog Dis. 2020 Apr 1;78(3):
pubmed: 32510562
Lancet. 2020 Jul 25;396(10246):e6-e7
pubmed: 32653078
Lancet Infect Dis. 2020 Sep;20(9):1034-1042
pubmed: 32422204
Int J Epidemiol. 2021 Mar 3;50(1):50-61
pubmed: 33094320
Lancet Public Health. 2020 Sep;5(9):e475-e483
pubmed: 32745512
Mayo Clin Proc. 2020 Aug;95(8):1621-1631
pubmed: 32753137
PLoS Med. 2020 Oct 1;17(10):e1003358
pubmed: 33001967
Lancet Respir Med. 2020 Sep;8(9):885-894
pubmed: 32717210
Clin Infect Dis. 2020 Nov 19;71(16):2027-2034
pubmed: 32221519
PLoS Pathog. 2020 Sep 24;16(9):e1008817
pubmed: 32970782
Lancet Infect Dis. 2021 Feb;21(2):168
pubmed: 32553187
Emerg Infect Dis. 2021 Jan;27(1):
pubmed: 33021927
Cochrane Database Syst Rev. 2020 Jun 25;6:CD013652
pubmed: 32584464
JAMA. 2020 Jun 16;323(23):2424-2425
pubmed: 32407440
Nat Med. 2020 Jun;26(6):845-848
pubmed: 32350462
J Clin Virol. 2020 Sep;130:104575
pubmed: 32805631
Nat Microbiol. 2020 Dec;5(12):1598-1607
pubmed: 33106674

Auteurs

Aya Mostafa (A)

Department of Community, Environmental, and Occupational Medicine, Faculty of Medicine, Ain Shams University, Cairo, Egypt. Electronic address: aya.kamaleldin@med.asu.edu.eg.

Sahar Kandil (S)

Department of Community, Environmental, and Occupational Medicine, Faculty of Medicine, Ain Shams University, Cairo, Egypt.

Manal H El-Sayed (MH)

Department of Pediatrics, Faculty of Medicine, Ain Shams University, Cairo, Egypt; Clinical Research Center (MASRI-CRC), Faculty of Medicine, Ain Shams University, Cairo, Egypt.

Samia Girgis (S)

Department of Clinical Pathology, Faculty of Medicine, Ain Shams University, Cairo, Egypt.

Hala Hafez (H)

Department of Clinical Pathology, Faculty of Medicine, Ain Shams University, Cairo, Egypt.

Mostafa Yosef (M)

Department of Community, Environmental, and Occupational Medicine, Faculty of Medicine, Ain Shams University, Cairo, Egypt.

Saly Saber (S)

Department of Clinical Pathology, Faculty of Medicine, Ain Shams University, Cairo, Egypt.

Hoda Ezzelarab (H)

Department of Clinical Pathology, Faculty of Medicine, Ain Shams University, Cairo, Egypt.

Marwa Ramadan (M)

Department of Clinical Pathology, Faculty of Medicine, Ain Shams University, Cairo, Egypt.

Eman Algohary (E)

Department of Clinical Pathology, Faculty of Medicine, Ain Shams University, Cairo, Egypt; Ain Shams University Specialized Hospitals, Egypt.

Gehan Fahmy (G)

Department of Clinical Pathology, Faculty of Medicine, Ain Shams University, Cairo, Egypt; Ain Shams University Specialized Hospitals, Egypt.

Iman Afifi (I)

Infection Control Unit, Ain Shams University Hospitals, Cairo, Egypt.

Fatmaelzahra Hassan (F)

Infection Control Unit, Ain Shams University Hospitals, Cairo, Egypt.

Shaimaa Elsayed (S)

Infection Control Unit, Ain Shams University Hospitals, Cairo, Egypt.

Amira Reda (A)

Infection Control Unit, Ain Shams University Hospitals, Cairo, Egypt.

Doaa Fattuh (D)

Infection Control Unit, Ain Shams University Hospitals, Cairo, Egypt.

Asmaa Mahmoud (A)

Infection Control Unit, Ain Shams University Hospitals, Cairo, Egypt.

Amany Mansour (A)

Infection Control Unit, Ain Shams University Hospitals, Cairo, Egypt.

Moshira Sabry (M)

Infection Control Unit, Ain Shams University Hospitals, Cairo, Egypt.

Petra Habeb (P)

Infection Control Unit, Ain Shams University Hospitals, Cairo, Egypt.

Fatma Se Ebeid (FS)

Department of Pediatrics, Faculty of Medicine, Ain Shams University, Cairo, Egypt; Clinical Research Center (MASRI-CRC), Faculty of Medicine, Ain Shams University, Cairo, Egypt.

Ali Elanwar (A)

Ain Shams University Specialized Hospitals, Egypt; Department of General Surgery, Faculty of Medicine, Ain Shams University, Cairo, Egypt.

Ayman Saleh (A)

Department of Cardiology, Faculty of Medicine, Ain Shams University, Cairo, Egypt.

Ossama Mansour (O)

Department of Otolaryngology, Head and Neck Surgery, Faculty of Medicine, Ain Shams University, Cairo, Egypt.

Ashraf Omar (A)

Department of Internal Medicine, Faculty of Medicine, Ain Shams University, Cairo, Egypt.

Mahmoud El-Meteini (M)

Department of Hepatobiliary Surgery & Liver Transplantation, Ain Shams Center for Organ Transplantation (ASCOT), Faculty of Medicine, Ain Shams University, Cairo, Egypt.

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