Generation of a Crigler-Najjar Syndrome Type I patient-derived induced pluripotent stem cell line CNS705 (HHUUKDi005-A).

Cell Differentiation Cell Line Cellular Reprogramming Crigler-Najjar Syndrome Embryoid Bodies Fibroblasts Humans Induced Pluripotent Stem Cells Kruppel-Like Factor 4

Journal

Stem cell research
ISSN: 1876-7753
Titre abrégé: Stem Cell Res
Pays: England
ID NLM: 101316957

Informations de publication

Date de publication:
03 2021
Historique:
received: 27 11 2020
revised: 15 12 2020
accepted: 29 12 2020
pubmed: 24 1 2021
medline: 22 6 2021
entrez: 23 1 2021
Statut: ppublish

Résumé

Human fibroblasts cells from a Crigler-Najjar Syndrome (CNS) patient were used to generate integration-free induced pluripotent stem cells (iPSCs) by over-expressing episomal-based plasmids expressing OCT4, SOX2, NANOG, KLF4, c-MYC and LIN28. The derived CNS705-iPSC line is homozygous for the UGT1A1 c.877_890delTACATTAATGCTTCinsA mutation. Pluripotency was confirmed by the expression of associated markers and embryoid body-based differentiation into cell types from all three germ layers. Comparative transcriptome analysis of the iPSC and the human embryonic stem cell line H9 revealed a Pearson's correlation of 0.9468.

Identifiants

DOI: 10.1016/j.scr.2021.102167 PMID: 33485181
pubmed: 33485181
pii: S1873-5061(21)00013-1
doi: 10.1016/j.scr.2021.102167
pii:
doi:

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

102167

Informations de copyright

Copyright © 2021 The Author(s). Published by Elsevier B.V. All rights reserved.

Auteurs

Nina Graffmann (N)

Institute for Stem Cell Research and Regenerative Medicine, Medical Faculty, Heinrich-Heine-University Düsseldorf, Moorenstr. 5, 40225 Düsseldorf, Germany.

Soraia Martins (S)

Institute for Stem Cell Research and Regenerative Medicine, Medical Faculty, Heinrich-Heine-University Düsseldorf, Moorenstr. 5, 40225 Düsseldorf, Germany.

Tijana Ljubikj (T)

Institute for Stem Cell Research and Regenerative Medicine, Medical Faculty, Heinrich-Heine-University Düsseldorf, Moorenstr. 5, 40225 Düsseldorf, Germany.

Julie Carole Matte (JC)

Institute for Stem Cell Research and Regenerative Medicine, Medical Faculty, Heinrich-Heine-University Düsseldorf, Moorenstr. 5, 40225 Düsseldorf, Germany.

Martina Bohndorf (M)

Institute for Stem Cell Research and Regenerative Medicine, Medical Faculty, Heinrich-Heine-University Düsseldorf, Moorenstr. 5, 40225 Düsseldorf, Germany.

Wasco Wruck (W)

Institute for Stem Cell Research and Regenerative Medicine, Medical Faculty, Heinrich-Heine-University Düsseldorf, Moorenstr. 5, 40225 Düsseldorf, Germany.

James Adjaye (J)

Institute for Stem Cell Research and Regenerative Medicine, Medical Faculty, Heinrich-Heine-University Düsseldorf, Moorenstr. 5, 40225 Düsseldorf, Germany. Electronic address: james.adjaye@med.uni-duesseldorf.de.

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Classifications MeSH

questionsmedicales.fr › Phénomènes physiologiques cellulaires › Différenciation cellulaire › Generation of a Crigler-Najjar Syndrome Type I...
questionsmedicales.fr › Cellules › Cellules cultivées › Lignée cellulaire › Generation of a Crigler-Najjar Syndrome Type I...
questionsmedicales.fr › Phénomènes génétiques › Reprogrammation cellulaire › Generation of a Crigler-Najjar Syndrome Type I...
questionsmedicales.fr › Maladies métaboliques et nutritionnelles › Maladies métaboliques › Erreurs innées du métabolisme › Hyperbilirubinémie héréditaire › Syndrome de Crigler-Najjar › Generation of a Crigler-Najjar Syndrome Type I...
questionsmedicales.fr › Cellules › Cellules souches › Cellules souches pluripotentes › Cellules souches embryonnaires › Corps embryoïdes › Generation of a Crigler-Najjar Syndrome Type I...
questionsmedicales.fr › Cellules › Cellules du tissu conjonctif › Fibroblastes › Generation of a Crigler-Najjar Syndrome Type I...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Primates › Haplorhini › Catarrhini › Hominidae › Humains › Generation of a Crigler-Najjar Syndrome Type I...
questionsmedicales.fr › Cellules › Cellules souches › Cellules souches pluripotentes › Cellules souches pluripotentes induites › Generation of a Crigler-Najjar Syndrome Type I...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Facteurs de transcription › Facteurs de transcription Krüppel-like › Facteur-4 de type Kruppel › Generation of a Crigler-Najjar Syndrome Type I...