Macromolecular aging: ATP hydrolysis-driven functional and structural changes in Escherichia coli RecQ helicase.

ATP hydrolysis Fatigued enzyme Macromolecular aging Non-fatigued enzyme RecQ helicase

Journal

Biochemical and biophysical research communications
ISSN: 1090-2104
Titre abrégé: Biochem Biophys Res Commun
Pays: United States
ID NLM: 0372516

Informations de publication

Date de publication:
20 Jan 2021
Historique:
received: 28 12 2020
accepted: 06 01 2021
pubmed: 24 1 2021
medline: 24 1 2021
entrez: 23 1 2021
Statut: aheadofprint

Résumé

Aging has been considered a phenomenon that can be only applied to cells or organisms. Here, we show that RecQ helicase from E. coli displays an aging phenomenon: this macromolecular motor loses its structure and function after hydrolyzing a certain number of ATP molecules. The aging process was only triggered by repeated catalytic cycles. These observations lead to a new concept: macromolecule aging.

Identifiants

pubmed: 33485211
pii: S0006-291X(21)00055-3
doi: 10.1016/j.bbrc.2021.01.020
pii:
doi:

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

29-33

Informations de copyright

Copyright © 2021 Elsevier Inc. All rights reserved.

Déclaration de conflit d'intérêts

Declaration of competing interest The authors declare no conflict of interest.

Auteurs

Dan Li (D)

College of Life Sciences, Northwest A&F University, Yangling, Shaanxi, 712100, China.

Hou-Qiang Xu (HQ)

Key Laboratory of Animal Genetics Breeding and Production in the Plateau Mountains Region, Ministry of Education, Guizhou University, Guiyang, 550025, China.

Xu-Guang Xi (XG)

College of Life Sciences, Northwest A&F University, Yangling, Shaanxi, 712100, China; The LBPA, Ecole Normale Supérieure Paris-Saclay, CNRS, Université Paris Saclay, Gif-sur-Yvette, France. Electronic address: xxi01@ens-cachan.fr.

Classifications MeSH