Exploring a possibility of using Raman spectroscopy for detection of Lyme disease.

Borrelia burgdorferi Lyme borreliosis Raman spectroscopy blood diagnostic test specimens

Journal

Journal of biophotonics
ISSN: 1864-0648
Titre abrégé: J Biophotonics
Pays: Germany
ID NLM: 101318567

Informations de publication

Date de publication:
05 2021
Historique:
revised: 18 01 2021
received: 02 12 2020
accepted: 19 01 2021
pubmed: 25 1 2021
medline: 13 7 2021
entrez: 24 1 2021
Statut: ppublish

Résumé

Lyme disease (LD), one of the most prevalent tick-borne diseases in the United States (US), is caused by Borreliella burgdorferi sensu stricto (Bb). To date, in the US, LD diagnostics is primarily based on validated two-tiered serological testing, which overall exhibits low sensitivity among other drawbacks. In the present study, a potential of Raman spectroscopy (RS) to detect Bb infection in mice has been explored. For that, C3H mice were infected with wild-type Bb strains, 297, B31, or B31-derived mutant, ∆vlsE. Blood samples taken prior to and post Bb infection were subjected to RS. The data demonstrated that RS did not directly detect Bb spirochetes in blood, but rather sensed biochemical changes associated with Bb infection. Despite Bb infection-associated blood changes detectable by RS were very limited, the partial least square discriminant analysis showed that the average true positive rates were 86% for 297 and 89% for B31 and ∆vlsE.

Identifiants

pubmed: 33486893
doi: 10.1002/jbio.202000477
doi:

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

e202000477

Informations de copyright

© 2021 Wiley-VCH GmbH.

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Auteurs

Charles Farber (C)

Department of Biochemistry and Biophysics, Texas A&M University, College Station, Texas, USA.

Rohini Morey (R)

Department of Biochemistry and Biophysics, Texas A&M University, College Station, Texas, USA.

Mark Krimmer (M)

Department of Biochemistry and Biophysics, Texas A&M University, College Station, Texas, USA.

Dmitry Kurouski (D)

Department of Biochemistry and Biophysics, Texas A&M University, College Station, Texas, USA.

Artem S Rogovskyy (AS)

Department of Veterinary Pathobiology, College of Veterinary Medicine and Biomedical Sciences, Texas A&M University, College Station, Texas, USA.

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