Post-calving umbilical cord tissue offcut: A potential source for the isolation of bovine mesenchymal stem cells.
cattle
isolation
mesenchymal stem cells
umbilical cord tissue
Journal
Veterinary world
ISSN: 0972-8988
Titre abrégé: Vet World
Pays: India
ID NLM: 101504872
Informations de publication
Date de publication:
Dec 2020
Dec 2020
Historique:
received:
22
06
2020
accepted:
16
11
2020
entrez:
25
1
2021
pubmed:
26
1
2021
medline:
26
1
2021
Statut:
ppublish
Résumé
Veterinary health care is an emergent area in animal sciences and innovative therapeutic approaches happen to be imperative in the present days. In view of the importance of cattle health and production, it is necessary to take up contemporary approach of stem cell therapy in this sector also. This study aimed to standardize an explant culture method of bovine umbilical tissue offcut to isolate mesenchymal stem cells (MSCs) because considerable efforts are required for ensuring easy accessibility and availability of MSCs in bulk quantity, as well as in establishing and characterizing the cell lines. The umbilical cord (UC) tissue matrix offcut was collected after calving. A simplified A considerable exponential growth rate of the UC-derived cells was noticed. In addition to their confirmation as MSCs, the cells also exhibited plastic adherent property and maintained the spindle-shaped morphology throughout the This simplified method of isolation and culture of bovine multipotent MSCs from the UC offcut collected after calving could be extrapolated for the greater availability of the cells for prospective therapeutic applications.
Sections du résumé
BACKGROUND AND AIM
OBJECTIVE
Veterinary health care is an emergent area in animal sciences and innovative therapeutic approaches happen to be imperative in the present days. In view of the importance of cattle health and production, it is necessary to take up contemporary approach of stem cell therapy in this sector also. This study aimed to standardize an explant culture method of bovine umbilical tissue offcut to isolate mesenchymal stem cells (MSCs) because considerable efforts are required for ensuring easy accessibility and availability of MSCs in bulk quantity, as well as in establishing and characterizing the cell lines.
MATERIALS AND METHODS
METHODS
The umbilical cord (UC) tissue matrix offcut was collected after calving. A simplified
RESULTS
RESULTS
A considerable exponential growth rate of the UC-derived cells was noticed. In addition to their confirmation as MSCs, the cells also exhibited plastic adherent property and maintained the spindle-shaped morphology throughout the
CONCLUSION
CONCLUSIONS
This simplified method of isolation and culture of bovine multipotent MSCs from the UC offcut collected after calving could be extrapolated for the greater availability of the cells for prospective therapeutic applications.
Identifiants
pubmed: 33487997
doi: 10.14202/vetworld.2020.2772-2779
pii: Vetworld-13-2772
pmc: PMC7811551
doi:
Types de publication
Journal Article
Langues
eng
Pagination
2772-2779Informations de copyright
Copyright: © Debbarma, et al.
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