Measuring protein levels in planarians using western blotting.

Animals Blotting, Western Helminth Proteins / metabolism Planarians / metabolism Regeneration
Model organisms Molecular biology Protein biochemistry

Journal

STAR protocols
ISSN: 2666-1667
Titre abrégé: STAR Protoc
Pays: United States
ID NLM: 101769501

Informations de publication

Date de publication:
19 03 2021
Historique:
entrez: 25 1 2021
pubmed: 26 1 2021
medline: 26 1 2021
Statut: epublish

Résumé

In the planarian field, two techniques are mostly used for protein detection: immunohistochemistry (IHC) and western blotting. While IHC is great for visualizing the spatial distribution of proteins in whole organisms, it has limitations in antibody availability and issues related to nonspecific expression. The use of western blotting can circumvent nonspecific expression, providing a dependable way to quantify proteins of interest. Here, we present a standardized, easily reproducible protocol with details on protein extractions of whole planarians and western blotting. For complete details on the use and execution of this protocol, please refer to Ziman et al. (2020a).

Identifiants

DOI: 10.1016/j.xpro.2020.100274 PMID: 33490988 PMC: PMC7811048
pubmed: 33490988
doi: 10.1016/j.xpro.2020.100274
pii: S2666-1667(20)30261-6
pmc: PMC7811048
doi:

Substances chimiques

Helminth Proteins 0

Types de publication

Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

Langues

eng

Pagination

100274

Subventions

Organisme : NIGMS NIH HHS
ID : R01 GM132753
Pays : United States

Informations de copyright

© 2021 The Authors.

Déclaration de conflit d'intérêts

The authors declare no competing interests.

Références

Nat Methods. 2012 Jul;9(7):671-5
pubmed: 22930834
J Cell Sci. 2020 May 27;133(10):
pubmed: 32265271
iScience. 2020 Oct 09;23(11):101665
pubmed: 33134895

Auteurs

Benjamin Ziman (B)

Department of Molecular & Cell Biology, University of California, Merced, CA, USA.
Quantitative and Systems Biology Graduate Program, University of California, Merced, CA, USA.

Néstor J Oviedo (NJ)

Department of Molecular & Cell Biology, University of California, Merced, CA, USA.
Quantitative and Systems Biology Graduate Program, University of California, Merced, CA, USA.
Health Sciences Research Institute, University of California, Merced, CA, USA.

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Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › Measuring protein levels in planarians using...
questionsmedicales.fr › Techniques d'investigation › Techniques de sonde moléculaire › Dosage immunologique › Immunotransfert › Technique de Western › Measuring protein levels in planarians using...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines d'helminthes › Measuring protein levels in planarians using...
questionsmedicales.fr › Eucaryotes › Animaux › Invertébrés › Helminthes › Plathelminthes › Turbellaria › Planaires › Measuring protein levels in planarians using...
questionsmedicales.fr › Phénomènes biologiques › Régénération › Measuring protein levels in planarians using...