Whole blood can be used as an alternative to isolated peripheral blood mononuclear cells to measure in vitro specific T-cell responses in human samples.
Adolescent
Adult
Blood
/ immunology
CD4-Positive T-Lymphocytes
/ immunology
Cytokines
/ analysis
Feasibility Studies
Female
Flow Cytometry
/ methods
Healthy Volunteers
Hepatitis B Vaccines
/ administration & dosage
Humans
Immunogenicity, Vaccine
Male
Middle Aged
Specimen Handling
/ methods
Tuberculosis Vaccines
/ administration & dosage
Vaccines, Subunit
/ administration & dosage
Young Adult
Cell-mediated immunity
Flow cytometry
Intracellular cytokine staining
Peripheral blood mononuclear cells
T-cell responses
Whole blood sample
Journal
Journal of immunological methods
ISSN: 1872-7905
Titre abrégé: J Immunol Methods
Pays: Netherlands
ID NLM: 1305440
Informations de publication
Date de publication:
05 2021
05 2021
Historique:
received:
05
05
2020
revised:
30
10
2020
accepted:
07
12
2020
pubmed:
26
1
2021
medline:
25
9
2021
entrez:
25
1
2021
Statut:
ppublish
Résumé
Vaccinology is confronted with diseases for which the control of T-cell responses by the vaccine is essential. Among the assays that have been designed to assess T-cell responses, intracellular cytokine staining (ICS) combined with flow cytometry is well-suited in the frame of clinical trials. This assay can be used starting from isolated peripheral blood mononuclear cells (PBMC) or from whole blood (WB), but firm equivalence between the two sample preparation methods has yet to be established. Therefore, we compared both methods by analyzing the frequency of antigen-specific CD4
Identifiants
pubmed: 33493551
pii: S0022-1759(20)30234-9
doi: 10.1016/j.jim.2020.112940
pii:
doi:
Substances chimiques
Cytokines
0
Hepatitis B Vaccines
0
Tuberculosis Vaccines
0
Vaccines, Subunit
0
Banques de données
ClinicalTrials.gov
['NCT00397943', 'NCT00805389']
Types de publication
Clinical Trial, Phase I
Clinical Trial, Phase II
Journal Article
Multicenter Study
Randomized Controlled Trial
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
112940Informations de copyright
Copyright © 2021 GlaxoSmithKline Biologicals SA. Published by Elsevier B.V. All rights reserved.