Regulation of Fibroblast Activation Protein by Transforming Growth Factor Beta-1 in Glioblastoma Microenvironment.
Cell Line, Tumor
Cells, Cultured
Endopeptidases
/ genetics
Fluorescent Antibody Technique
Gene Expression Regulation, Neoplastic
/ drug effects
Glioblastoma
/ etiology
Humans
Immunohistochemistry
Membrane Proteins
/ genetics
Phosphorylation
Transforming Growth Factor beta1
/ metabolism
Tumor Microenvironment
/ drug effects
Smad2
fibroblast activation protein
glioblastoma
regulation of expression
seprase
signaling
transforming growth factor beta
tumor microenvironment
Journal
International journal of molecular sciences
ISSN: 1422-0067
Titre abrégé: Int J Mol Sci
Pays: Switzerland
ID NLM: 101092791
Informations de publication
Date de publication:
21 Jan 2021
21 Jan 2021
Historique:
received:
01
12
2020
revised:
12
01
2021
accepted:
18
01
2021
entrez:
26
1
2021
pubmed:
27
1
2021
medline:
7
9
2021
Statut:
epublish
Résumé
The proline-specific serine protease fibroblast activation protein (FAP) can participate in the progression of malignant tumors and represents a potential diagnostic and therapeutic target. Recently, we demonstrated an increased expression of FAP in glioblastomas, particularly those of the mesenchymal subtype. Factors controlling FAP expression in glioblastomas are unknown, but evidence suggests that transforming growth factor beta (TGFbeta) can trigger mesenchymal changes in these tumors. Here, we investigated whether TGFbeta promotes FAP expression in transformed and stromal cells constituting the glioblastoma microenvironment. We found that both FAP and TGFbeta-1 are upregulated in glioblastomas and display a significant positive correlation. We detected TGFbeta-1 immunopositivity broadly in glioblastoma tissues, including tumor parenchyma regions in the immediate vicinity of FAP-immunopositive perivascular stromal cells. Wedemonstrate for the first time that TGFbeta-1 induces expression of FAP in non-stem glioma cells, pericytes, and glioblastoma-derived endothelial and FAP
Identifiants
pubmed: 33494271
pii: ijms22031046
doi: 10.3390/ijms22031046
pmc: PMC7864518
pii:
doi:
Substances chimiques
Membrane Proteins
0
TGFB1 protein, human
0
Transforming Growth Factor beta1
0
Endopeptidases
EC 3.4.-
fibroblast activation protein alpha
EC 3.4.21.-
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Ministerstvo Zdravotnictví Ceské Republiky
ID : 15-31379A
Organisme : Ministerstvo Školství, Mládeže a Tělovýchovy
ID : CZ.02.1.01/0.0/0.0/16_019/0000785
Organisme : Ministerstvo Školství, Mládeže a Tělovýchovy
ID : LM2015064
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