Newcastle Disease Virus-Like Particles Displaying Prefusion-Stabilized SARS-CoV-2 Spikes Elicit Potent Neutralizing Responses.
Newcastle disease virus
S-2P stabilization
SARS-CoV-2
VLP
neutralizing antibody
prefusion-stabilized spike
vaccine
virus-like particle
Journal
Vaccines
ISSN: 2076-393X
Titre abrégé: Vaccines (Basel)
Pays: Switzerland
ID NLM: 101629355
Informations de publication
Date de publication:
21 Jan 2021
21 Jan 2021
Historique:
received:
20
12
2020
revised:
15
01
2021
accepted:
16
01
2021
entrez:
26
1
2021
pubmed:
27
1
2021
medline:
27
1
2021
Statut:
epublish
Résumé
The COVID-19 pandemic highlights an urgent need for vaccines that confer protection from SARS-CoV-2 infection. One approach to an effective COVID-19 vaccine may be through the display of SARS-CoV-2 spikes on the surface of virus-like particles, in a manner structurally mimicking spikes on a native virus. Here we report the development of Newcastle disease virus-like particles (NDVLPs) displaying the prefusion-stabilized SARS-CoV-2 spike ectodomain (S2P). Immunoassays with SARS-CoV-2-neutralizing antibodies revealed the antigenicity of S2P-NDVLP to be generally similar to that of soluble S2P, and negative-stain electron microscopy showed S2P on the NDVLP surface to be displayed with a morphology corresponding to its prefusion conformation. Mice immunized with S2P-NDVLP showed substantial neutralization titers (geometric mean ID
Identifiants
pubmed: 33494381
pii: vaccines9020073
doi: 10.3390/vaccines9020073
pmc: PMC7912142
pii:
doi:
Types de publication
Journal Article
Langues
eng
Subventions
Organisme : Vaccine Research Center
ID : ZIA AI005149-01
Références
N Engl J Med. 2020 Oct 15;383(16):1544-1555
pubmed: 32722908
Nature. 2020 Oct;586(7830):567-571
pubmed: 32756549
Vaccines (Basel). 2020 Dec 17;8(4):
pubmed: 33348607
Nat Med. 2021 Feb;27(2):279-288
pubmed: 33335322
Immunity. 2020 Oct 13;53(4):724-732.e7
pubmed: 32783919
Nature. 2020 Aug;584(7819):115-119
pubmed: 32454513
Science. 2020 Sep 25;369(6511):1586-1592
pubmed: 32694201
Nature. 2020 Oct;586(7830):516-527
pubmed: 32967006
Immunity. 2020 Jun 16;52(6):971-977.e3
pubmed: 32413330
Lancet. 2020 Nov 14;396(10262):1595-1606
pubmed: 33065034
N Engl J Med. 2020 Dec 31;383(27):2603-2615
pubmed: 33301246
Nat Commun. 2020 Dec 16;11(1):6402
pubmed: 33328475
J Virol. 2014 Sep 1;88(17):10165-76
pubmed: 24965456
Nature. 2020 Dec;588(7838):498-502
pubmed: 32805734
Nature. 2020 Aug;584(7821):437-442
pubmed: 32555388
Science. 2020 Mar 13;367(6483):1260-1263
pubmed: 32075877
Proc Natl Acad Sci U S A. 2017 Aug 29;114(35):E7348-E7357
pubmed: 28807998
Ann N Y Acad Sci. 1995 Nov 27;772:30-9
pubmed: 8546411
Hum Vaccin Immunother. 2021 Jun 3;17(6):1635-1649
pubmed: 33270478
Nat Med. 2021 Feb;27(2):270-278
pubmed: 33335323
Sci Rep. 2020 Oct 23;10(1):18149
pubmed: 33097791
J Virol. 2006 Nov;80(22):11062-73
pubmed: 16971425
Cell. 2020 Nov 25;183(5):1367-1382.e17
pubmed: 33160446
Sci Immunol. 2020 Jun 11;5(48):
pubmed: 32527802
N Engl J Med. 2020 Nov 12;383(20):1920-1931
pubmed: 32663912
Sci Adv. 2021 Mar 19;7(12):
pubmed: 33741598
Nature. 2020 Aug;584(7821):450-456
pubmed: 32698192
Sci Rep. 2017 May 19;7(1):2193
pubmed: 28526819
Science. 2020 Jul 3;369(6499):77-81
pubmed: 32376603
Wiley Interdiscip Rev Nanomed Nanobiotechnol. 2011 Mar-Apr;3(2):174-196
pubmed: 20872839
J Virol. 2011 Dec;85(23):12201-15
pubmed: 21937658
PLoS Pathog. 2021 Apr 21;17(4):e1009064
pubmed: 33882114
Cell. 2020 Oct 29;183(3):730-738.e13
pubmed: 32979942
Semin Immunol. 2017 Dec;34:123-132
pubmed: 28887001
NPJ Vaccines. 2020 Oct 16;5:98
pubmed: 33088593
Vaccine. 2005 Mar 18;23(17-18):2273-9
pubmed: 15755610
Science. 2020 Jun 12;368(6496):1274-1278
pubmed: 32404477
NPJ Vaccines. 2020 Mar 6;5(1):18
pubmed: 32194995
Proc Natl Acad Sci U S A. 2020 May 26;117(21):11727-11734
pubmed: 32376634
Cell Host Microbe. 2020 Sep 9;28(3):465-474.e4
pubmed: 32798445
Science. 2020 May 29;368(6494):948-950
pubmed: 32393526
Lancet. 2020 Jun 13;395(10240):1845-1854
pubmed: 32450106
Cardiovasc Pathol. 2021 Jan - Feb;50:107278
pubmed: 32889088
N Biotechnol. 2017 Oct 25;39(Pt B):174-180
pubmed: 28778817
Nature. 2020 Apr;580(7805):576-577
pubmed: 32346146
PLoS Med. 2006 Dec;3(12):e525
pubmed: 17194199
J Biol Chem. 2021 Jan-Jun;296:100111
pubmed: 33229438
Nature. 2020 Aug;584(7821):443-449
pubmed: 32668443
Nat Commun. 2020 Aug 14;11(1):4081
pubmed: 32796842
Virus Res. 2021 Apr 2;295:198305
pubmed: 33482242
Lancet Infect Dis. 2021 Feb;21(2):181-192
pubmed: 33217362
Cell Rep. 2020 Oct 27;33(4):108322
pubmed: 33091382
Curr Protoc Microbiol. 2013 Oct 02;30:18.2.1-18.2.21
pubmed: 24510891
Science. 2020 Aug 14;369(6505):806-811
pubmed: 32434945
Front Immunol. 2018 Sep 19;9:1963
pubmed: 30283434