Oxidative Stress and Gene Expression Modifications Mediated by Extracellular Vesicles: An In Vivo Study of the Radiation-Induced Bystander Effect.
DNA damage repair
antioxidant enzymes
apoptosis
extracellular vesicles
gene expression
ionising radiation
lipid peroxidation
oxidative damage
Journal
Antioxidants (Basel, Switzerland)
ISSN: 2076-3921
Titre abrégé: Antioxidants (Basel)
Pays: Switzerland
ID NLM: 101668981
Informations de publication
Date de publication:
21 Jan 2021
21 Jan 2021
Historique:
received:
18
12
2020
revised:
15
01
2021
accepted:
16
01
2021
entrez:
26
1
2021
pubmed:
27
1
2021
medline:
27
1
2021
Statut:
epublish
Résumé
Radiation-induced bystander effect is a biological response in nonirradiated cells receiving signals from cells exposed to ionising radiation. The aim of this in vivo study was to analyse whether extracellular vesicles (EVs) originating from irradiated mice could induce modifications in the redox status and expression of radiation-response genes in bystander mice. C57BL/6 mice were whole-body irradiated with 0.1-Gy and 2-Gy X-rays, and EVs originating from mice irradiated with the same doses were injected into naïve, bystander mice. Lipid peroxidation in the spleen and plasma reactive oxygen metabolite (ROM) levels increased 24 h after irradiation with 2 Gy. The expression of antioxidant enzyme genes and inducible nitric oxide synthase 2 (iNOS2) decreased, while cell cycle arrest-, senescence- and apoptosis-related genes were upregulated after irradiation with 2 Gy. In bystander mice, no significant alterations were observed in lipid peroxidation or in the expression of genes connected to cell cycle arrest, senescence and apoptosis. However, there was a systemic increase in the circulating ROM level after an intravenous EV injection, and EVs originating from 2-Gy-irradiated mice caused a reduced expression of antioxidant enzyme genes and iNOS2 in bystander mice. In conclusion, we showed that ionising radiation-induced alterations in the cellular antioxidant system can be transmitted in vivo in a bystander manner through EVs originating from directly irradiated animals.
Identifiants
pubmed: 33494540
pii: antiox10020156
doi: 10.3390/antiox10020156
pmc: PMC7911176
pii:
doi:
Types de publication
Journal Article
Langues
eng
Subventions
Organisme : Euratom Research and Training Program 2014-2018
ID : 662287
Organisme : Hungarian National Research, Development and Innovation Office
ID : NKFI-124879
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