Recombinant MVA-prime elicits neutralizing antibody responses by inducing antigen-specific B cells in the germinal center.


Journal

NPJ vaccines
ISSN: 2059-0105
Titre abrégé: NPJ Vaccines
Pays: England
ID NLM: 101699863

Informations de publication

Date de publication:
25 Jan 2021
Historique:
received: 30 07 2020
accepted: 07 12 2020
entrez: 26 1 2021
pubmed: 27 1 2021
medline: 27 1 2021
Statut: epublish

Résumé

The RV144 HIV-1 vaccine trial has been the only clinical trial to date that has shown any degree of efficacy and associated with the presence of vaccine-elicited HIV-1 envelope-specific binding antibody and CD4+ T-cell responses. This trial also showed that a vector-prime protein boost combined vaccine strategy was better than when used alone. Here we have studied three different priming vectors-plasmid DNA, recombinant MVA, and recombinant VSV, all encoding clade C transmitted/founder Env 1086 C gp140, for priming three groups of six non-human primates each, followed by a protein boost with adjuvanted 1086 C gp120 protein. Our data showed that MVA-priming favors the development of higher antibody binding titers and neutralizing activity compared with other vectors. Analyses of the draining lymph nodes revealed that MVA-prime induced increased germinal center reactivity characterized by higher frequencies of germinal center (PNA

Identifiants

pubmed: 33495459
doi: 10.1038/s41541-020-00277-1
pii: 10.1038/s41541-020-00277-1
pmc: PMC7835239
doi:

Types de publication

Journal Article

Langues

eng

Pagination

15

Subventions

Organisme : U.S. Department of Health & Human Services | NIH | National Institute of Allergy and Infectious Diseases (NIAID)
ID : UM1-AI-100645
Organisme : U.S. Department of Health & Human Services | NIH | National Institute of Allergy and Infectious Diseases (NIAID)
ID : P30-AI060354
Organisme : U.S. Department of Health & Human Services | NIH | National Institute of Allergy and Infectious Diseases (NIAID)
ID : OD011103
Organisme : Bill and Melinda Gates Foundation (Bill & Melinda Gates Foundation)
ID : OPP1032325

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Auteurs

Leila Eslamizar (L)

Center for Virology and Vaccine Research, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA, USA.
Integrative Toxicology, Nonclinical Drug Safety, Boehringer Ingelheim Pharmaceuticals, Inc., 175 Briar Ridge Road, Ridgefield, CT, 06877, USA.

Constantinos Petrovas (C)

Vaccine Research Center, NIAID, NIH, Bethesda, MD, USA. Konstantinos.Petrovas@chuv.ch.
Institute of Pathology, Department of Laboratory Medicine and Pathology, Lausanne University Hospital and Lausanne University, Lausanne, Switzerland. Konstantinos.Petrovas@chuv.ch.

David J Leggat (DJ)

Vaccine Research Center, NIAID, NIH, Bethesda, MD, USA.

Kathryn Furr (K)

Center for Virology and Vaccine Research, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA, USA.

Michelle L Lifton (ML)

Center for Virology and Vaccine Research, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA, USA.

Gail Levine (G)

Foundation for the National Institutes of Health, Bethesda, MD, USA.

Steven Ma (S)

Vaccine Research Center, NIAID, NIH, Bethesda, MD, USA.

Christopher Fletez-Brant (C)

Vaccine Research Center, NIAID, NIH, Bethesda, MD, USA.

Wesley Hoyland (W)

Vaccine Research Center, NIAID, NIH, Bethesda, MD, USA.

Madhu Prabhakaran (M)

Vaccine Research Center, NIAID, NIH, Bethesda, MD, USA.

Sandeep Narpala (S)

Vaccine Research Center, NIAID, NIH, Bethesda, MD, USA.

Kristin Boswell (K)

Vaccine Research Center, NIAID, NIH, Bethesda, MD, USA.

Takuya Yamamoto (T)

Vaccine Research Center, NIAID, NIH, Bethesda, MD, USA.

Hua-Xin Liao (HX)

Foundation for the National Institutes of Health, Bethesda, MD, USA.

David Pickup (D)

Duke Human Vaccine Institute, Durham, NC, USA.

Elizabeth Ramsburg (E)

Duke Human Vaccine Institute, Durham, NC, USA.

Laura Sutherland (L)

Duke Human Vaccine Institute, Durham, NC, USA.

Adrian McDermott (A)

Vaccine Research Center, NIAID, NIH, Bethesda, MD, USA.

Mario Roederer (M)

Vaccine Research Center, NIAID, NIH, Bethesda, MD, USA.

David Montefiori (D)

Duke Human Vaccine Institute, Durham, NC, USA.

Richard A Koup (RA)

Vaccine Research Center, NIAID, NIH, Bethesda, MD, USA.

Barton F Haynes (BF)

Duke Human Vaccine Institute, Durham, NC, USA.

Norman L Letvin (NL)

Center for Virology and Vaccine Research, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA, USA.

Sampa Santra (S)

Center for Virology and Vaccine Research, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA, USA. ssantra@bidmc.harvard.edu.

Classifications MeSH