BRD2 inhibition blocks SARS-CoV-2 infection by reducing transcription of the host cell receptor ACE2.

Journal

bioRxiv : the preprint server for biology
Titre abrégé: bioRxiv
Pays: United States
ID NLM: 101680187

Informations de publication

Date de publication:
20 Sep 2021
Historique:
pubmed: 28 1 2021
medline: 28 1 2021
entrez: 27 1 2021
Statut: epublish

Résumé

SARS-CoV-2 infection of human cells is initiated by the binding of the viral Spike protein to its cell-surface receptor ACE2. We conducted a targeted CRISPRi screen to uncover druggable pathways controlling Spike protein binding to human cells. We found that the protein BRD2 is required for

Identifiants

DOI: 10.1101/2021.01.19.427194 PMID: 33501440 PMC: PMC7836110
pubmed: 33501440
doi: 10.1101/2021.01.19.427194
pmc: PMC7836110
pii:
doi:

Types de publication

Preprint

Langues

eng

Subventions

Organisme : NIA NIH HHS
ID : F32 AG063487
Pays : United States

Commentaires et corrections

Type : UpdateIn

Déclaration de conflit d'intérêts

Competing Interests: JCS, JO, TM and KH are employees and shareholders of Synthego Corporation.

Auteurs

Avi J Samelson (AJ)

Institute for Neurodegenerative Diseases, Department of Biochemistry and Biophysics, University of California, San Francisco, San Francisco, CA 94158, USA.
Chan-Zuckerberg Biohub, San Francisco, CA 94158, USA.

Quang Dinh Tran (QD)

Institut Pasteur, Viral Populations and Pathogenesis Unit, CNRS UMR 3569, 75015 Paris, France.
École Doctorale BioSPC, Université de Paris, Sorbonne Paris Cité, 75006 Paris, France.

Rémy Robinot (R)

Institut Pasteur, CIVIC Group, Virus and Immunity Unit, CNRS UMR 3569, 75015 Paris, France.

Lucia Carrau (L)

Department of Microbiology, Icahn School of Medicine, New York, NY 10029.

Veronica V Rezelj (VV)

Institut Pasteur, Viral Populations and Pathogenesis Unit, CNRS UMR 3569, 75015 Paris, France.

Alice Mac Kain (A)

Institut Pasteur, Viral Populations and Pathogenesis Unit, CNRS UMR 3569, 75015 Paris, France.
École Doctorale BioSPC, Université de Paris, Sorbonne Paris Cité, 75006 Paris, France.

Merissa Chen (M)

Institute for Neurodegenerative Diseases, Department of Biochemistry and Biophysics, University of California, San Francisco, San Francisco, CA 94158, USA.
Chan-Zuckerberg Biohub, San Francisco, CA 94158, USA.

Gokul N Ramadoss (GN)

Gladstone Institutes, San Francisco, 94158, CA, USA.
Biomedical Sciences PhD Program, University of California, San Francisco, CA, USA.

Xiaoyan Guo (X)

Institute for Neurodegenerative Diseases, Department of Biochemistry and Biophysics, University of California, San Francisco, San Francisco, CA 94158, USA.
Chan-Zuckerberg Biohub, San Francisco, CA 94158, USA.

Shion A Lim (SA)

Department of Pharmaceutical Chemistry, University of California San Francisco, San Francisco, California, 94158, USA.
Present address: Department of Antibody Engineering, Genentech Inc., South San Francisco, CA, 94080, USA.

Irene Lui (I)

Department of Pharmaceutical Chemistry, University of California San Francisco, San Francisco, California, 94158, USA.

James Nunez (J)

Department of Cellular and Molecular Pharmacology, University of California, San Francisco, CA 94158, USA.
Howard Hughes Medical Institute, University of California, San Francisco, CA 94158, USA.

Sarah J Rockwood (SJ)

Gladstone Institutes, San Francisco, 94158, CA, USA.

Jianhui Wang (J)

School of Medicine, Southern University of Science and Technology, Shenzhen, China 518055.

Na Liu (N)

School of Medicine, Southern University of Science and Technology, Shenzhen, China 518055.

Jared Carlson-Stevermer (J)

Synthego Corporation, Redwood City, CA 94063, USA, Department of Biology, Massachusetts Institute of Technology, Cambridge, 02142, USA.

Jennifer Oki (J)

Synthego Corporation, Redwood City, CA 94063, USA, Department of Biology, Massachusetts Institute of Technology, Cambridge, 02142, USA.

Travis Maures (T)

Synthego Corporation, Redwood City, CA 94063, USA, Department of Biology, Massachusetts Institute of Technology, Cambridge, 02142, USA.

Kevin Holden (K)

Synthego Corporation, Redwood City, CA 94063, USA, Department of Biology, Massachusetts Institute of Technology, Cambridge, 02142, USA.

Jonathan S Weissman (JS)

Department of Cellular and Molecular Pharmacology, University of California, San Francisco, CA 94158, USA.
Howard Hughes Medical Institute, University of California, San Francisco, CA 94158, USA.
Whitehead Institute for Biomedical Research, Cambridge, 02142, USA, Innovative Genomics Institute, Berkeley, 94720, CA, USA.

James A Wells (JA)

Chan-Zuckerberg Biohub, San Francisco, CA 94158, USA.
Department of Pharmaceutical Chemistry, University of California San Francisco, San Francisco, California, 94158, USA.
Department of Cellular and Molecular Pharmacology, University of California, San Francisco, CA 94158, USA.

Bruce R Conklin (BR)

Gladstone Institutes, San Francisco, 94158, CA, USA.
Department of Cellular and Molecular Pharmacology, University of California, San Francisco, CA 94158, USA.
Department of Ophthalmology, University of California, San Francisco, San Francisco, CA. 94158, USA.
Department of Medicine, University of California, San Francisco, San Francisco, CA, 94158, USA.

Benjamin R TenOever (BR)

Department of Microbiology, Icahn School of Medicine, New York, NY 10029.

Lisa A Chakrabarti (LA)

Institut Pasteur, CIVIC Group, Virus and Immunity Unit, CNRS UMR 3569, 75015 Paris, France.

Marco Vignuzzi (M)

Institut Pasteur, Viral Populations and Pathogenesis Unit, CNRS UMR 3569, 75015 Paris, France.

Ruilin Tian (R)

Institute for Neurodegenerative Diseases, Department of Biochemistry and Biophysics, University of California, San Francisco, San Francisco, CA 94158, USA.
Chan-Zuckerberg Biohub, San Francisco, CA 94158, USA.
School of Medicine, Southern University of Science and Technology, Shenzhen, China 518055.

Martin Kampmann (M)

Institute for Neurodegenerative Diseases, Department of Biochemistry and Biophysics, University of California, San Francisco, San Francisco, CA 94158, USA.
Chan-Zuckerberg Biohub, San Francisco, CA 94158, USA.
Department of Biochemistry and Biophysics, University of California, San Francisco, San Francisco, CA, 94158, USA.

Classifications MeSH