The Middle Part of the Plucked Hair Follicle Outer Root Sheath Is Identified as an Area Rich in Lineage-Specific Stem Cell Markers.
hair follicle
in vitro cultivation
mesenchymal stem cells
multipotency
neuroectodermal cells
non-invasive sampling
outer root sheath
Journal
Biomolecules
ISSN: 2218-273X
Titre abrégé: Biomolecules
Pays: Switzerland
ID NLM: 101596414
Informations de publication
Date de publication:
25 01 2021
25 01 2021
Historique:
received:
13
12
2020
revised:
15
01
2021
accepted:
19
01
2021
entrez:
28
1
2021
pubmed:
29
1
2021
medline:
23
7
2021
Statut:
epublish
Résumé
Hair follicle outer root sheath (ORS) is a putative source of stem cells with therapeutic capacity. ORS contains several multipotent stem cell populations, primarily in the distal compartment of the bulge region. However, the bulge is routinely obtained using invasive isolation methods, which require human scalp tissue ex vivo. Non-invasive sampling has been standardized by means of the plucking procedure, enabling to reproducibly obtain the mid-ORS part. The mid-ORS shows potential for giving rise to multiple stem cell populations in vitro. To demonstrate the phenotypic features of distal, middle, and proximal ORS parts, gene and protein expression profiles were studied in physically separated portions. The mid-part of the ORS showed a comparable or higher NGFR, nestin/
Identifiants
pubmed: 33503918
pii: biom11020154
doi: 10.3390/biom11020154
pmc: PMC7912066
pii:
doi:
Substances chimiques
Biomarkers
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Deutsche Forschungsgemeinschaft
ID : SFB TRR 67 B12 to VS
Organisme : Deutsche Forschungsgemeinschaft
ID : SFB TRR 67 B3 to SF and JCS
Organisme : Sächsisches Staatsministerium für Wissenschaft und Kunst
ID : SIKT to VS
Organisme : Department of Cranial Maxillofacial Plastic Surgery, University Hospital Leipzig
ID : Chair to BL
Références
Proc Natl Acad Sci U S A. 2005 Dec 6;102(49):17734-8
pubmed: 16314569
Clin Exp Dermatol. 2006 Nov;31(6):807-12
pubmed: 16981909
J Vis Exp. 2013 Apr 06;(74):
pubmed: 23608752
Br J Dermatol. 2008 Sep;159(3):646-52
pubmed: 18637899
Cytotherapy. 2006;8(4):315-7
pubmed: 16923606
Methods Mol Biol. 2014;1210:203-27
pubmed: 25173171
Cell Tissue Res. 2015 Oct;362(1):69-86
pubmed: 25948482
Br J Cancer. 1996 Nov;74(10):1586-91
pubmed: 8932339
J Invest Dermatol. 2010 May;130(5):1227-36
pubmed: 19829300
Proc Natl Acad Sci U S A. 2003 Aug 19;100(17):9958-61
pubmed: 12904579
Dev Cell. 2005 Dec;9(6):855-61
pubmed: 16326396
Exp Dermatol. 2010 Jun;19(6):543-5
pubmed: 20374294
J Clin Invest. 2006 Jan;116(1):249-60
pubmed: 16395407
Cell. 2011 Jul 22;146(2):334-334.e2
pubmed: 21784251
Am J Pathol. 2006 Jun;168(6):1879-88
pubmed: 16723703
Int J Mol Med. 2013 Apr;31(4):913-21
pubmed: 23403715
Lab Invest. 2009 Aug;89(8):844-56
pubmed: 19506554
Ann N Y Acad Sci. 2009 Sep;1176:124-34
pubmed: 19796240
Exp Hematol. 2002 Jul;30(7):783-91
pubmed: 12135677
Proc Natl Acad Sci U S A. 2004 Sep 7;101(36):13291-5
pubmed: 15331785
J Invest Dermatol. 2003 Nov;121(5):1220; author reply 1220-1
pubmed: 14708630
Iran J Public Health. 2017 Sep;46(9):1167-1175
pubmed: 29026781
Exp Dermatol. 2012 Dec;21(12):948-50
pubmed: 23171457
Cytotherapy. 2019 Oct;21(10):1019-1024
pubmed: 31526643
Cell Cycle. 2015;14(14):2362-6
pubmed: 25970547
J Am Acad Dermatol. 2003 Feb;48(2):207-14
pubmed: 12582390
Br J Dermatol. 2009 Sep;161(3):678-82
pubmed: 19438446
Stem Cells Int. 2012;2012:975871
pubmed: 22666272
Haematologica. 2010 Apr;95(4):651-9
pubmed: 20179086
PLoS One. 2013 Oct 04;8(10):e76979
pubmed: 24124603
Nature. 2010 Apr 1;464(7289):686-7
pubmed: 20360726
Proc Natl Acad Sci U S A. 2005 Apr 12;102(15):5530-4
pubmed: 15802470
World J Stem Cells. 2015 Mar 26;7(2):470-6
pubmed: 25815130
Stem Cells. 2012 May;30(5):876-87
pubmed: 22311737
Cell Stem Cell. 2008 Sep 11;3(3):301-13
pubmed: 18786417
Cells. 2020 Sep 10;9(9):
pubmed: 32927740
Methods Mol Biol. 2016;1453:49-55
pubmed: 27431246
Science. 2010 Mar 12;327(5971):1385-9
pubmed: 20223988
Stem Cells. 2006 Apr;24(4):928-35
pubmed: 16306150
Blood. 2009 Jan 22;113(4):816-26
pubmed: 18818395
J Immunol. 2000 Nov 1;165(9):5262-8
pubmed: 11046060
Sci Rep. 2018 Jun 18;8(1):9321
pubmed: 29915318