Decrease in Cellular Nanovesicles Concentration in Blood of Athletes More Than 15 Hours After Marathon.
Adult
Athletes
Blood
/ metabolism
Blood Cell Count
C-Reactive Protein
/ analysis
Erythrocytes
/ metabolism
Female
Flow Cytometry
Humans
Interleukin-6
/ blood
Lipids
/ chemistry
Male
Marathon Running
Middle Aged
Nanoparticles
/ chemistry
Physical Exertion
/ physiology
Tumor Necrosis Factor-alpha
/ blood
Young Adult
blood samples
cellular nanovesicles
inflammation process
marathon
membrane vesiculation
physical effort
Journal
International journal of nanomedicine
ISSN: 1178-2013
Titre abrégé: Int J Nanomedicine
Pays: New Zealand
ID NLM: 101263847
Informations de publication
Date de publication:
2021
2021
Historique:
received:
16
09
2020
accepted:
12
11
2020
entrez:
28
1
2021
pubmed:
29
1
2021
medline:
12
2
2021
Statut:
epublish
Résumé
Cellular nanovesicles (CNVs), that are shed from cells, have been recognized as promising indicators of health status. We analyzed the effect of long-distance running on concentration of CNVs, along with some standard blood parameters, in 27 athletes two days before and >15 hours after physical effort. CNVs were isolated by repetitive centrifugation and washing of samples, and assessed by flow cytometry. Cholinesterase (ChE) and glutathione S-transferase (GST) activity were measured spectrophotometrically. Interleukin 6 (IL-6) and tumor necrosis factor-α (TNF-α) concentrations were measured using enzyme-linked immunosorbent assay (ELISA). C-reactive protein (CRP) was measured with immunoturbidimetric determination and lipidogram parameters were measured by enzymatic colorimetric assay. Flow cytometry was used for blood cell count and mean platelet volume (MPV) measurement. More than 15 hours after physical effort a decrease was found in CNVs' concentration in isolates from blood (46%; p<0.05), in ChE activity in whole blood (47%; p<0.001), in plasma (34%; p<0.01), and in erythrocyte suspension (54%; p<0.001), as well as in GST activity in erythrocyte suspension (16%; p<0.01) and in IL-6 concentration in plasma (63%; p<0.05). We found no change in GST activity in plasma and in TNF-α concentration in plasma. Correlations (>0.8; p<0.001) between CNVs' concentration and ChE activity, and GST activity, respectively, in erythrocyte suspension were found. We found that >15 hours post-physical effort, CNVs' concentration was below the initial value, concomitant with other measured parameters: ChE and GST activity as well as IL-6 concentration, indicating a favorable effect of physical effort on health status. CNVs' concentration and ChE activity in isolates from peripheral blood proved to have potential as indicators of the response of the human body to inflammation after physical effort. Physical activity should be considered as an important factor in preparation of subjects for blood sampling in procedures focusing on CNV-containing diagnostic and therapeutic compounds.
Identifiants
pubmed: 33505159
doi: 10.2147/IJN.S282200
pii: 282200
pmc: PMC7829122
doi:
Substances chimiques
Interleukin-6
0
Lipids
0
Tumor Necrosis Factor-alpha
0
C-Reactive Protein
9007-41-4
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
443-456Informations de copyright
© 2021 Jan et al.
Déclaration de conflit d'intérêts
No conflicts of interest, financial or otherwise, are declared by the authors.
Références
Epigenet Insights. 2018 Dec 12;11:2516865718818839
pubmed: 30560231
Biomed Res Int. 2020 Apr 18;2020:6381396
pubmed: 32382562
Cells. 2019 Sep 06;8(9):
pubmed: 31500151
Int J Environ Health Res. 2012;22(3):279-86
pubmed: 22149082
J Appl Physiol (1985). 2005 Apr;98(4):1154-62
pubmed: 15772055
Med Sci Sports Exerc. 2011 Jul;43(7):1169-76
pubmed: 21131870
Br J Haematol. 2015 Dec;171(5):854-61
pubmed: 26456230
J Gerontol A Biol Sci Med Sci. 2006 Jun;61(6):575-84
pubmed: 16799139
Biochem Genet. 2016 Aug;54(4):544-551
pubmed: 27368697
J Extracell Vesicles. 2015 Jul 02;4:28239
pubmed: 26142461
Am J Physiol Heart Circ Physiol. 2016 Nov 1;311(5):H1297-H1310
pubmed: 27638881
Clin Hemorheol Microcirc. 2011;47(1):15-25
pubmed: 21321404
Stem Cells. 2020 Feb;38(2):291-300
pubmed: 31675158
Lipids Health Dis. 2011 Mar 21;10:47
pubmed: 21418650
Cell Metab. 2018 Jan 9;27(1):237-251.e4
pubmed: 29320704
J Pharm Bioallied Sci. 2011 Jan;3(1):118-27
pubmed: 21430962
J Leukoc Biol. 2008 Nov;84(5):1271-8
pubmed: 18664531
Small. 2018 Sep;14(39):e1801650
pubmed: 30160371
Mediators Inflamm. 2019 Apr 17;2019:9213074
pubmed: 31148950
Electrophoresis. 2020 Apr;41(7-8):449-470
pubmed: 31967658
Cardiovasc Diabetol. 2017 Sep 19;16(1):118
pubmed: 28927403
J Biol Chem. 1987 Jul 5;262(19):9412-20
pubmed: 3597417
J Extracell Vesicles. 2019 Jul 03;8(1):1628592
pubmed: 31303981
Arch Dis Child Fetal Neonatal Ed. 1999 Sep;81(2):F130-3
pubmed: 10448182
Cytometry A. 2016 Feb;89(2):123-34
pubmed: 26651033
J Physiol. 2004 Jul 15;558(Pt 2):633-45
pubmed: 15169848
J Pathol. 2008 Jan;214(2):149-60
pubmed: 18161752
Eur J Appl Physiol. 2011 Jun;111(6):1035-45
pubmed: 21088973
Int J Nanomedicine. 2011;6:2737-48
pubmed: 22128248
Virol J. 2010 Sep 16;7:234
pubmed: 20846372
Cell. 2010 Dec 10;143(6):875-87
pubmed: 21145455
J Extracell Vesicles. 2019 May 28;8(1):1615820
pubmed: 31191831
Life Sci. 2020 Jun 1;250:117585
pubmed: 32243928
Oncotarget. 2018 Mar 30;9(24):17181-17198
pubmed: 29682215
J Immunol Methods. 2008 Sep 30;338(1-2):21-30
pubmed: 18675270
Eur J Appl Physiol Occup Physiol. 1992;64(4):354-8
pubmed: 1592062
Biochem Pharmacol. 1961 Jul;7:88-95
pubmed: 13726518
Antioxidants (Basel). 2017 Sep 28;6(4):
pubmed: 28956814
Biomed Res Int. 2018 Mar 26;2018:5670145
pubmed: 29780825
Crit Rev Immunol. 2002;22(3):165-82
pubmed: 12498381
J Appl Physiol (1985). 2017 Apr 1;122(4):1031-1039
pubmed: 28153942
J Diabetes Res. 2018 Jun 19;2018:7807245
pubmed: 30018986
Adv Enzymol Relat Areas Mol Biol. 1985;57:357-417
pubmed: 3898742
Free Radic Biol Med. 2020 Mar;149:44-50
pubmed: 31783096
Circ Res. 2014 Jan 3;114(1):109-13
pubmed: 24141170
J Med Biochem. 2015 Apr;34(2):179-190
pubmed: 28356830
Eur J Biochem. 2000 Jan;267(2):583-90
pubmed: 10632729
J Extracell Vesicles. 2019 Aug 1;8(1):1648167
pubmed: 31489144
Nutrients. 2019 Mar 08;11(3):
pubmed: 30857250
Eur J Pharm Sci. 2017 Feb 15;98:17-29
pubmed: 27737793
Rheumatology (Oxford). 2017 Mar 1;56(3):408-416
pubmed: 28031442