Diagnostic system for the detection of severe fever with thrombocytopenia syndrome virus RNA from suspected infected animals.
Animals
Antibodies, Viral
/ immunology
Bunyaviridae Infections
/ virology
Cats
/ virology
Diagnostic Tests, Routine
/ methods
Dogs
Enzyme-Linked Immunosorbent Assay
/ methods
Female
Fever
/ diagnosis
Hemorrhagic Fevers, Viral
/ diagnosis
Immunoglobulin G
/ immunology
Immunoglobulin M
/ immunology
Japan
Male
Phlebovirus
/ genetics
RNA, Viral
/ blood
Reverse Transcriptase Polymerase Chain Reaction
/ methods
Severe Fever with Thrombocytopenia Syndrome
/ diagnosis
Thrombocytopenia
/ diagnosis
Journal
PloS one
ISSN: 1932-6203
Titre abrégé: PLoS One
Pays: United States
ID NLM: 101285081
Informations de publication
Date de publication:
2021
2021
Historique:
received:
03
09
2020
accepted:
04
12
2020
entrez:
28
1
2021
pubmed:
29
1
2021
medline:
1
5
2021
Statut:
epublish
Résumé
Severe fever with thrombocytopenia syndrome virus (SFTSV) causes severe hemorrhagic fever in humans and cats. Clinical symptoms of SFTS-infected cats resemble those of SFTS patients, whereas SFTS-contracted cats have high levels of viral RNA loads in the serum and body fluids. Due to the risk of direct infection from SFTS-infected cats to human, it is important to diagnose SFTS-suspected animals. In this study, a reverse transcription polymerase chain reaction (RT-PCR) was newly developed to diagnose SFTS-suspected animals without non-specific reactions. Four primer sets were newly designed from consensus sequences constructed from 108 strains of SFTSV. A RT-PCR with these four primer sets successfully and specifically detected four clades of SFTSV. Their limits of detection are 1-10 copies/reaction. Using this RT-PCR, 5 cat cases among 56 SFTS-suspected animal cases were diagnosed as SFTS. From these cats, IgM or IgG against SFTSV were detected by enzyme-linked immunosorbent assay (ELISA), but not neutralizing antibodies by plaque reduction neutralization titer (PRNT) test. This phenomenon is similar to those of fatal SFTS patients. This newly developed RT-PCR could detect SFTSV RNA of several clades and from SFTS-suspected animals. In addition to ELISA and PRNT test, the useful laboratory diagnosis systems of SFTS-suspected animals has been made in this study.
Sections du résumé
BACKGROUND
Severe fever with thrombocytopenia syndrome virus (SFTSV) causes severe hemorrhagic fever in humans and cats. Clinical symptoms of SFTS-infected cats resemble those of SFTS patients, whereas SFTS-contracted cats have high levels of viral RNA loads in the serum and body fluids. Due to the risk of direct infection from SFTS-infected cats to human, it is important to diagnose SFTS-suspected animals. In this study, a reverse transcription polymerase chain reaction (RT-PCR) was newly developed to diagnose SFTS-suspected animals without non-specific reactions.
METHODOLOGY/PRINCIPLE FINDINGS
Four primer sets were newly designed from consensus sequences constructed from 108 strains of SFTSV. A RT-PCR with these four primer sets successfully and specifically detected four clades of SFTSV. Their limits of detection are 1-10 copies/reaction. Using this RT-PCR, 5 cat cases among 56 SFTS-suspected animal cases were diagnosed as SFTS. From these cats, IgM or IgG against SFTSV were detected by enzyme-linked immunosorbent assay (ELISA), but not neutralizing antibodies by plaque reduction neutralization titer (PRNT) test. This phenomenon is similar to those of fatal SFTS patients.
CONCLUSION/SIGNIFICANCE
This newly developed RT-PCR could detect SFTSV RNA of several clades and from SFTS-suspected animals. In addition to ELISA and PRNT test, the useful laboratory diagnosis systems of SFTS-suspected animals has been made in this study.
Identifiants
pubmed: 33507990
doi: 10.1371/journal.pone.0238671
pii: PONE-D-20-25871
pmc: PMC7842937
doi:
Substances chimiques
Antibodies, Viral
0
Immunoglobulin G
0
Immunoglobulin M
0
RNA, Viral
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e0238671Déclaration de conflit d'intérêts
The authors have declared that no competing interests exist.
Références
J Infect Dis. 2014 Mar;209(6):816-27
pubmed: 24231186
N Engl J Med. 2011 Apr 21;364(16):1523-32
pubmed: 21410387
Sci Rep. 2019 Aug 19;9(1):11990
pubmed: 31427690
J Infect Dis. 2015 Sep 15;212(6):889-98
pubmed: 25762790
Emerg Infect Dis. 2013 May;19(5):756-63
pubmed: 23648209
J Infect Chemother. 2018 Oct;24(10):802-806
pubmed: 30017796
Emerg Infect Dis. 2019 May;25(5):1029-1031
pubmed: 31002059
Medicine (Baltimore). 2016 Jan;95(4):e2533
pubmed: 26825892
Ticks Tick Borne Dis. 2017 Jun;8(4):626-630
pubmed: 28442241
Rev Med Virol. 2014 Mar;24(2):90-102
pubmed: 24310908
Emerg Infect Dis. 2013 Nov;19(11):1892-4
pubmed: 24206586
Ticks Tick Borne Dis. 2017 Jan;8(1):9-12
pubmed: 27542506
Emerg Infect Dis. 2018 Sep;24(9):1726-1729
pubmed: 30124411
J Clin Microbiol. 2014 Sep;52(9):3325-33
pubmed: 24989600
Emerg Microbes Infect. 2020 Dec;9(1):148-151
pubmed: 31918622
BMC Bioinformatics. 2012 Jun 18;13:134
pubmed: 22708584
Jpn J Infect Dis. 2014;67(1):1-4
pubmed: 24451093
Mol Biol Evol. 2016 Jul;33(7):1870-4
pubmed: 27004904