Subclinical effects of adapalene-benzoyl peroxide: a prospective in vivo imaging study on acne micromorphology and transfollicular delivery.
Journal
Journal of the European Academy of Dermatology and Venereology : JEADV
ISSN: 1468-3083
Titre abrégé: J Eur Acad Dermatol Venereol
Pays: England
ID NLM: 9216037
Informations de publication
Date de publication:
Jun 2021
Jun 2021
Historique:
received:
28
08
2020
accepted:
14
01
2021
pubmed:
29
1
2021
medline:
21
5
2021
entrez:
28
1
2021
Statut:
ppublish
Résumé
Adapalene-benzoyl peroxide (A-BPO) is a first-line topical treatment for acne vulgaris. In vivo reflectance confocal microscopy (RCM) and optical coherence tomography (OCT) detect micromorphological changes over time and visualize transfollicular delivery. To visualize temporal, subclinical effects of A-BPO on acne micromorphology using RCM and OCT, and evaluate their impact on transfollicular delivery of microparticulate carrier systems. Fifteen patients with mild to moderate acne received a 6-week course of A-BPO. Micromorphological changes were evaluated at time 0, 3 and 6 weeks with RCM (n = 1190 images) and OCT (n = 210 scans). Transfollicular delivery of microparticles was assessed at baseline and week 6. In vivo imaging visualized steady normalization of skin micromorphology in response to A-BPO over 6 weeks, including decreased hyperkeratinization of follicular borders (RCM median decrease -71.2%, P < 0.05), reduced intrafollicular keratinous content (RCM median decrease -47.7%, P < 0.05) and increased epidermal thickness (OCT median increase of 25.25%, P < 0.05). Imaging visualized microparticles in the follicular unit. Despite a visible reduction in keratin and sebum, transfollicular microparticle delivery appeared unaffected. Reflectance confocal microscopy and OCT detect A-BPO-induced changes in micromorphology and visualize transfollicular microparticle delivery. Keratolysis and sebolysis did not have a measurable effect on transfollicular delivery of microparticles.
Sections du résumé
BACKGROUND
BACKGROUND
Adapalene-benzoyl peroxide (A-BPO) is a first-line topical treatment for acne vulgaris. In vivo reflectance confocal microscopy (RCM) and optical coherence tomography (OCT) detect micromorphological changes over time and visualize transfollicular delivery.
OBJECTIVES
OBJECTIVE
To visualize temporal, subclinical effects of A-BPO on acne micromorphology using RCM and OCT, and evaluate their impact on transfollicular delivery of microparticulate carrier systems.
METHODS
METHODS
Fifteen patients with mild to moderate acne received a 6-week course of A-BPO. Micromorphological changes were evaluated at time 0, 3 and 6 weeks with RCM (n = 1190 images) and OCT (n = 210 scans). Transfollicular delivery of microparticles was assessed at baseline and week 6.
RESULTS
RESULTS
In vivo imaging visualized steady normalization of skin micromorphology in response to A-BPO over 6 weeks, including decreased hyperkeratinization of follicular borders (RCM median decrease -71.2%, P < 0.05), reduced intrafollicular keratinous content (RCM median decrease -47.7%, P < 0.05) and increased epidermal thickness (OCT median increase of 25.25%, P < 0.05). Imaging visualized microparticles in the follicular unit. Despite a visible reduction in keratin and sebum, transfollicular microparticle delivery appeared unaffected.
CONCLUSIONS
CONCLUSIONS
Reflectance confocal microscopy and OCT detect A-BPO-induced changes in micromorphology and visualize transfollicular microparticle delivery. Keratolysis and sebolysis did not have a measurable effect on transfollicular delivery of microparticles.
Substances chimiques
Dermatologic Agents
0
Drug Combinations
0
Gels
0
Adapalene
1L4806J2QF
Benzoyl Peroxide
W9WZN9A0GM
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1377-1385Informations de copyright
© 2021 European Academy of Dermatology and Venereology.
Références
Williams HC, Dellavalle RP, Garner S. Acne vulgaris. Lancet (London, England) 2012; 379: 361-372.
Moradi Tuchayi S, Makrantonaki E, Ganceviciene R, Dessinioti C, Feldman SR, Zouboulis CC. Acne vulgaris. Nat Rev Dis Primers 2015; 1: 15029.
Bhate K, Williams HC. Epidemiology of acne vulgaris. Br J Dermatol 2013; 168: 474-485.
Nast A, Dréno B, Bettoli V et al. European evidence-based (S3) guideline for the treatment of acne - update 2016 - short version. J Eur Acad Dermatol Venereol 2016; 30: 1261-1268.
Nast A, Dréno B, Bettoli V et al. European evidence-based (S3) guidelines for the treatment of acne. J Eur Acad Dermatol Venereol 2012; 26: 1-29.
Zhou R, Jiang X. Effects of adapalene-benzoyl peroxide combination gel in treatment or maintenance therapy of moderate or severe acne vulgaris: a meta-analysis. Ann Dermatol 2014; 26: 43-52.
Hauk L. Acne vulgaris: treatment guidelines from the AAD. Am Fam Physician 2017; 95: 740-741.
Dutil M. Benzoyl peroxide: enhancing antibiotic efficacy in acne management. Skin Therapy Lett 2010; 15: 5-7.
Yang Z, Zhang Y, Mosler EL et al. Topical benzoyl peroxide for acne. Cochrane Database Syst Rev 2020: 1-587.
Thiboutot DM, Weiss J, Bucko A et al. Adapalene-benzoyl peroxide, a fixed-dose combination for the treatment of acne vulgaris: Results of a multicenter, randomized double-blind, controlled study. J Am Acad Dermatol 2007; 57: 791-799.
Chourasia R, Jain SK. Drug targeting through pilosebaceous route. Curr Drug Targets 2009; 10: 950-967.
Rancan F, Vogt A. Getting under the skin: what is the potential of the transfollicular route in drug delivery? Ther Deliv 2014; 5: 875-877.
Patzelt A, Lademann J. Recent advances in follicular drug delivery of nanoparticles. Expert Opin Drug Deliv 2020; 17: 49-60.
Jeff W, Theresa C, Liu J-C. A sebum dissolving microgel complex enhances targeted delivery in different acne treatment formulations. J Am Acad Dermatol 2007; 56: AB22.
Meidan VM. Methods for quantifying intrafollicular drug delivery: a critical appraisal. Expert Opin Drug Deliv 2010; 7: 1095-1108.
Jain SK, Verma A, Jain A, Hurkat P. Transfollicular drug delivery: current perspectives. Res Rep Transdermal Drug Deliv 2016; 5: 1.
Lademann J, Otberg N, Jacobi U, Hoffman RM, Blume-Peytavi U. Follicular penetration and targeting. J Investig Dermatol Symp Proc 2005; 10: 301-303.
Lademann J, Otberg N, Richter H et al. Investigation of follicular penetration of topically applied substances. Skin Pharmacol Appl Skin Physiol 2001; 14(SUPPL. 1): 17-22.
Paithankar D, Hwang BH, Munavalli G et al. Ultrasonic delivery of silica-gold nanoshells for photothermolysis of sebaceous glands in humans: nanotechnology from the bench to clinic. J Control Release 2015; 206: 30-36.
Paithankar DY, Sakamoto FH, Farinelli WA et al. Acne treatment based on selective photothermolysis of sebaceous follicles with topically delivered light-absorbing gold microparticles. J Invest Dermatol 2015; 135: 1727-1734.
Mogensen M, Bojesen S, Israelsen NM et al. Two optical coherence tomography systems detect topical gold nanoshells in hair follicles, sweat ducts and measure epidermis. J Biophotonics 2018; 11: e201700348.
Ring HC, Israelsen NM, Bang O, Haedersdal M, Mogensen M. Potential of contrast agents to enhance in vivo confocal microscopy and optical coherence tomography in dermatology: a review. J Biophotonics 2019; 12: e201800462.
Fuchs CSK, Ortner VK, Mogensen M, Philipsen PA, Haedersdal M. Transfollicular delivery of gold microparticles in healthy skin and acne vulgaris, assessed by in vivo reflectance confocal microscopy and optical coherence tomography. Lasers Surg Med 2019; 51: 430-438.
Gao SQ, Maeda T, Okano K, Palczewski K. A microparticle/hydrogel combination drug-delivery system for sustained release of retinoids. Investig Ophthalmol Vis Sci 2012; 53: 6314-6323.
Latter G, Grice JE, Mohammed Y, Roberts MS, Benson HAE. Targeted topical delivery of retinoids in the management of acne vulgaris: current formulations and novel delivery systems. Pharmaceutics 2019; 11: 490.
Manfredini M, Greco M, Farnetani F et al. In vivo monitoring of topical therapy for acne with reflectance confocal microscopy. Skin Res Technol 2017; 23: 36-40.
Banzhaf CA, Phothong W, Suku MH et al. Basal cell carcinoma treated with combined ablative fractional laser and ingenol mebutate - an exploratory study monitored by optical coherence tomography and reflectance confocal microscopy. J Eur Acad Dermatol Venereol 2020; 34: 502-509.
Guilera JM, Barreiro Capurro A, Carrera Alvárez C, Puig Sardá S. The role of reflectance confocal microscopy in clinical trials for tumor monitoring. Dermatol Clin 2016; 34: 519-526.
Schmitz L, Reinhold U, Bierhoff E, Dirschka T. Optical coherence tomography: its role in daily dermatological practice. J Dtsch Dermatol Ges 2013; 11: 499-507.
Fuchs CSK, Andersen AJB, Ardigo M, Philipsen PA, Haedersdal M, Mogensen M. Acne vulgaris severity graded by in vivo reflectance confocal microscopy and optical coherence tomography. Lasers Surg Med 2019; 51.
Themstrup L, Ciardo S, Manfredi M et al. In vivo, micro-morphological vascular changes induced by topical brimonidine studied by Dynamic optical coherence tomography. J Eur Acad Dermatol Venereol 2016; 30: 974-979.
Manfredini M, Greco M, Farnetani F et al. Acne: morphologic and vascular study of lesions and surrounding skin by means of optical coherence tomography. J Eur Acad Dermatol Venereol 2017; 31: 1541-1546.
Manfredini M, Mazzaglia G, Ciardo S et al. Acne: in vivo morphologic study of lesions and surrounding skin by means of reflectance confocal microscopy. J Eur Acad Dermatol Venereol 2015; 29: 933-939.
Schuh S, Holmes J, Ulrich M et al. Imaging blood vessel morphology in skin: dynamic optical coherence tomography as a novel potential diagnostic tool in dermatology. Dermatol Ther 2017; 7: 187-202.
Ulrich M, Themstrup L, de Carvalho N et al. Dynamic optical coherence tomography of skin blood vessels - proposed terminology and practical guidelines. J Eur Acad Dermatology Venereol 2018; 32: 152-155.
Shao Y, He T, Fisher GJ, Voorhees JJ, Quan T. Molecular basis of retinol anti-ageing properties in naturally aged human skin in vivo. Int J Cosmet Sci 2017; 39: 56-65.
Griffiths CE, Ancian P, Humphries J et al. Adapalene 0.1% gel and adapalene 0.1% cream stimulate retinoic acid receptor mediated gene transcription without significant irritative effects in the skin of healthy human volunteers. Br J Dermatol 1998; 139(Suppl 52): 12-16.
Lademann J, Richter H, Teichmann A et al. Nanoparticles - An efficient carrier for drug delivery into the hair follicles. Eur J Pharm Biopharm 2007; 66: 159-164.
Khlebtsov N, Dykman L. Biodistribution and toxicity of engineered gold nanoparticles: a review of in vitro and in vivo studies. Chem Soc Rev 2011; 40: 1647-1671.
Hirsch LR, Gobin AM, Lowery AR et al. Metal nanoshells. Ann Biomed Eng 2006; 34: 15-22.
Lademann J, Richter H, Schaefer UF et al. Hair follicles - a long-term reservoir for drug delivery. Skin Pharmacol Physiol 2006; 19: 232-236.
Bhatia AC, Clebus E, Rujna P, Miracki K. Long Term Follow-Up From European Post Market Study Evaluating Treatment of Acne Vulgaris Using Gold Microparticles and Laser in Subjects Pre Treated with Topical Adapalene/Benzoyl Peroxide [abstract]. 40th Annu Conf by Am Soc Laser Med Surg. Online, US.
Hansen FS, Wenande E, Haedersdal M, Fuchs CSK. Microneedle fractional radiofrequency-induced micropores evaluated by in vivo reflectance confocal microscopy, optical coherence tomography, and histology. Ski Res Technol 2019; 25: 482-488.
Flament F, Francois G, Qiu H et al. Facial skin pores: a multiethnic study. Clin Cosmet Investig Dermatol 2015; 8: 85-93.
Sugiyama-Nakagiri Y, Sugata K, Hachiya A, Osanai O, Ohuchi A, Kitahara T. Ethnic differences in the structural properties of facial skin. J Dermatol Sci 2009; 53: 135-139.
Cooper AJ, Harris VR. Modern management of acne. Med J Aust 2017; 206: 41-45.