Metabolic profile distinguishes laminitis-susceptible and -resistant ponies before and after feeding a high sugar diet.
Animals
Blood Glucose
/ analysis
Diet
/ veterinary
Dietary Carbohydrates
/ adverse effects
Disease Resistance
Disease Susceptibility
/ metabolism
Female
Foot Diseases
/ etiology
Glycerophospholipids
/ blood
Hoof and Claw
Horse Diseases
/ etiology
Horses
/ metabolism
Insulin
/ blood
Male
Metabolome
Risk Factors
Biomarker
Equine metabolic syndrome
Insulin dysregulation
Laminitis
Metabolome
Pituitary pars intermedia dysfunction
Journal
BMC veterinary research
ISSN: 1746-6148
Titre abrégé: BMC Vet Res
Pays: England
ID NLM: 101249759
Informations de publication
Date de publication:
28 Jan 2021
28 Jan 2021
Historique:
received:
14
09
2020
accepted:
13
01
2021
entrez:
29
1
2021
pubmed:
30
1
2021
medline:
3
9
2021
Statut:
epublish
Résumé
Insulin dysregulation (ID) is a key risk factor for equine endocrinopathic laminitis, but in many cases ID can only be assessed accurately using dynamic tests. The identification of other biomarkers could provide an alternative or adjunct diagnostic method, to allow early intervention before laminitis develops. The present study characterised the metabolome of ponies with varying degrees of ID using basal and postprandial plasma samples obtained during a previous study, which examined the predictive power of blood insulin levels for the development of laminitis, in ponies fed a high-sugar diet. Samples from 10 pre-laminitic (PL - subsequently developed laminitis) and 10 non-laminitic (NL - did not develop laminitis) ponies were used in a targeted metabolomic assay. Differential concentration and pathway analysis were performed using linear models and global tests. Significant changes in the concentration of six glycerophospholipids (adj. P ≤ 0.024) and a global enrichment of the glucose-alanine cycle (adj. P = 0.048) were found to characterise the response of PL ponies to the high-sugar diet. In contrast, the metabolites showed no significant association with the presence or absence of pituitary pars intermedia dysfunction in all ponies. The present results suggest that ID and laminitis risk are associated with alterations in the glycerophospholipid and glucose metabolism, which may help understand and explain some molecular processes causing or resulting from these conditions. The prognostic value of the identified biomarkers for laminitis remains to be investigated in further metabolomic trials in horses and ponies.
Sections du résumé
BACKGROUND
BACKGROUND
Insulin dysregulation (ID) is a key risk factor for equine endocrinopathic laminitis, but in many cases ID can only be assessed accurately using dynamic tests. The identification of other biomarkers could provide an alternative or adjunct diagnostic method, to allow early intervention before laminitis develops. The present study characterised the metabolome of ponies with varying degrees of ID using basal and postprandial plasma samples obtained during a previous study, which examined the predictive power of blood insulin levels for the development of laminitis, in ponies fed a high-sugar diet. Samples from 10 pre-laminitic (PL - subsequently developed laminitis) and 10 non-laminitic (NL - did not develop laminitis) ponies were used in a targeted metabolomic assay. Differential concentration and pathway analysis were performed using linear models and global tests.
RESULTS
RESULTS
Significant changes in the concentration of six glycerophospholipids (adj. P ≤ 0.024) and a global enrichment of the glucose-alanine cycle (adj. P = 0.048) were found to characterise the response of PL ponies to the high-sugar diet. In contrast, the metabolites showed no significant association with the presence or absence of pituitary pars intermedia dysfunction in all ponies.
CONCLUSIONS
CONCLUSIONS
The present results suggest that ID and laminitis risk are associated with alterations in the glycerophospholipid and glucose metabolism, which may help understand and explain some molecular processes causing or resulting from these conditions. The prognostic value of the identified biomarkers for laminitis remains to be investigated in further metabolomic trials in horses and ponies.
Identifiants
pubmed: 33509165
doi: 10.1186/s12917-021-02763-7
pii: 10.1186/s12917-021-02763-7
pmc: PMC7841998
doi:
Substances chimiques
Blood Glucose
0
Dietary Carbohydrates
0
Glycerophospholipids
0
Insulin
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
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