Decreased angiogenesis as a possible pathomechanism in cervical degenerative myelopathy.
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
28 01 2021
28 01 2021
Historique:
received:
19
02
2020
accepted:
07
01
2021
entrez:
29
1
2021
pubmed:
30
1
2021
medline:
15
9
2021
Statut:
epublish
Résumé
Endogenous immune mediated reactions of inflammation and angiogenesis are components of the spinal cord injury in patients with degenerative cervical myelopathy (DCM). The aim of this study was to identify alteration of certain mediators participating in angiogenetic and inflammatory reactions in patients with DCM. A consecutive series of 42 patients with DCM and indication for surgical decompression were enrolled for the study. 28 DCM patients were included, as CSF samples were taken preoperatively. We enrolled 42 patients requiring surgery for a thoracic abdominal aortic aneurysm (TAAA) as neurologically healthy controls. In 38 TAAA patients, CSF samples were taken prior to surgery and thus included. We evaluated the neurological status of patients and controls prior to surgery including NDI and mJOA. Protein-concentrations of factors with a crucial role in inflammation and angiogenesis were measured in CSF via ELISA testing (pg/ml): Angiopoietin 2, VEGF-A and C, RANTES, IL 1 beta and IL 8. Additionally, evaluated the status of the blood-spinal cord barrier (BSCB) by Reibers´diagnostic in all participants. Groups evidently differed in their neurological status (mJOA: DCM 10.1 ± 3.3, TAAA 17.3 ± 1.2, p < .001; NDI: DCM 47.4 ± 19.7, TAAA 5.3 ± 8.6, p < .001). There were no particular differences in age and gender distribution. However, we detected statistically significant differences in concentrations of mediators between the groups: Angiopoietin 2 (DCM 267.1.4 ± 81.9, TAAA 408.6 ± 177.1, p < .001) and VEGF C (DCM 152.2 ± 96.1, TAAA 222.4 ± 140.3, p = .04). DCM patients presented a mild to moderate BSCB disruption, controls had no signs of impairment. In patients with DCM, we measured decreased concentrations of angiogenic mediators. These results correspond to findings of immune mediated secondary harm in acute spinal cord injury. Reduced angiogenic activity could be a relevant part of the pathogenesis of DCM and secondary harm to the spinal cord.
Identifiants
pubmed: 33510227
doi: 10.1038/s41598-021-81766-8
pii: 10.1038/s41598-021-81766-8
pmc: PMC7843718
doi:
Substances chimiques
Cytokines
0
Types de publication
Clinical Trial
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
2497Références
Proc Natl Acad Sci U S A. 1999 Mar 2;96(5):1904-9
pubmed: 10051567
Spine (Phila Pa 1976). 2001 Sep 1;26(17):1890-4; discussion 1895
pubmed: 11568701
Nat Med. 1997 Jan;3(1):73-6
pubmed: 8986744
Science. 1989 Dec 8;246(4935):1306-9
pubmed: 2479986
Proc Natl Acad Sci U S A. 1995 Apr 11;92(8):3566-70
pubmed: 7724599
Mol Cell Biol. 2009 Apr;29(8):2011-22
pubmed: 19223473
CNS Neurol Disord Drug Targets. 2014;13(10):1654-66
pubmed: 25470401
Biochem Soc Trans. 2007 Nov;35(Pt 5):1122-6
pubmed: 17956293
Eur Spine J. 2017 Jan;26(1):78-84
pubmed: 27342612
Eur Spine J. 2020 May;29(5):986-993
pubmed: 31982957
Proc Natl Acad Sci U S A. 2014 Mar 18;111(11):E1035-42
pubmed: 24591593
J Immunol. 2014 Sep 1;193(5):2438-54
pubmed: 25049355
J Neurol Sci. 1994 Apr;122(2):189-203
pubmed: 8021703
Electroencephalogr Clin Neurophysiol. 1989 Jul-Aug;74(4):241-59
pubmed: 2471626
J Neurosci. 2001 Aug 1;21(15):5528-34
pubmed: 11466424
EMBO J. 1996 Jan 15;15(2):290-98
pubmed: 8617204
J Biol Chem. 2009 Aug 21;284(34):22680-9
pubmed: 19553662
Physiol Rev. 2002 Jul;82(3):673-700
pubmed: 12087132
Science. 1997 Jul 4;277(5322):55-60
pubmed: 9204896
Prog Neurobiol. 2017 May;152:181-199
pubmed: 27079786
Neuroscientist. 2013 Aug;19(4):409-21
pubmed: 23204243
Restor Neurol Neurosci. 2003;21(3-4):79-96
pubmed: 14530572
Spine (Phila Pa 1976). 1988 Jul;13(7):843-6
pubmed: 3057649
J Comp Neurol. 2002 Jun 24;448(2):203-16
pubmed: 12012430
Brain Behav Immun. 2003 Jun;17(3):152-7
pubmed: 12706413
Ocul Immunol Inflamm. 2007 May-Jun;15(3):275-8
pubmed: 17613841
Arterioscler Thromb Vasc Biol. 2012 Jun;32(6):1400-9
pubmed: 22556334
Neurol Res. 2004 Dec;26(8):870-83
pubmed: 15727271
J Neuropathol Exp Neurol. 1999 Mar;58(3):245-54
pubmed: 10197816
Jpn J Pharmacol. 2002 Mar;88(3):227-49
pubmed: 11949877
J Neurol Sci. 2001 Mar 1;184(2):101-22
pubmed: 11239944
Am J Pathol. 2001 Sep;159(3):893-903
pubmed: 11549582
J Clin Invest. 2000 Oct;106(7):829-38
pubmed: 11018070
Sci Rep. 2016 Oct 21;6:35190
pubmed: 27767064
Proc Natl Acad Sci U S A. 1998 Sep 1;95(18):10896-901
pubmed: 9724801
Brain Behav Immun. 2011 Feb;25(2):349-59
pubmed: 20974246
Curr Drug Metab. 2012 Jan;13(1):50-60
pubmed: 22292807
Science. 1983 Feb 25;219(4587):983-5
pubmed: 6823562
Invest Radiol. 1975 Nov-Dec;10(6):543-51
pubmed: 811584
Brain. 2011 May;134(Pt 5):1277-92
pubmed: 21490053
J Cell Physiol. 2008 Dec;217(3):809-18
pubmed: 18720385
Biochem Biophys Res Commun. 2008 Oct 24;375(3):287-91
pubmed: 18680722
Recent Pat CNS Drug Discov. 2008 Nov;3(3):189-99
pubmed: 18991808
Int J Neurosci. 2017 Jul;127(7):624-633
pubmed: 27412492
J Spinal Disord Tech. 2008 Apr;21(2):145-7
pubmed: 18391721
Exp Neurobiol. 2018 Aug;27(4):299-308
pubmed: 30181692
Spine (Phila Pa 1976). 2002 Oct 15;27(20):2205-10; discussion 2210-1
pubmed: 12394893
Cardiovasc Res. 2011 Jul 15;91(2):358-67
pubmed: 21436157
Neuroscience. 2012 Jan 27;202:454-64
pubmed: 22020092
Brain Res. 2012 Aug 30;1471:46-55
pubmed: 22820296
Brain. 2011 May;134(Pt 5):1259-61
pubmed: 21596766
Eur Spine J. 2009 Dec;18(12):1946-50
pubmed: 19543752
Spine (Phila Pa 1976). 2013 Oct 15;38(22 Suppl 1):S21-36
pubmed: 23963004
Clin Chem Lab Med. 2001 Apr;39(4):324-32
pubmed: 11388657
Cereb Cortex. 2011 Jul;21(7):1695-702
pubmed: 21127017
Mol Biol Cell. 1993 Dec;4(12):1317-26
pubmed: 8167412
Trends Pharmacol Sci. 2011 Oct;32(10):617-22
pubmed: 21788085
PLoS One. 2010 Feb 26;5(2):e9414
pubmed: 20195471
Eur Spine J. 2015 Apr;24 Suppl 2:132-8
pubmed: 24626958
J Neurosci Res. 2012 Oct;90(10):1873-82
pubmed: 22714747
Sci STKE. 2001 Dec 11;2001(112):re21
pubmed: 11741095
J Neurosurg. 1991 Jul;75(1):15-26
pubmed: 2045903
Int Angiol. 2015 Dec;34(6):520-8
pubmed: 25968806
Science. 1997 May 30;276(5317):1423-5
pubmed: 9162011
Exp Eye Res. 2004 Oct;79(4):553-61
pubmed: 15381039
Nat Med. 2003 Jun;9(6):669-76
pubmed: 12778165
Exp Neurol. 2008 Feb;209(2):378-88
pubmed: 17662717
Nat Immunol. 2004 Jan;5(1):74-80
pubmed: 14634646
Nature. 1995 Jul 6;376(6535):62-6
pubmed: 7596435
J Neurotrauma. 2007 Mar;24(3):492-507
pubmed: 17402855
J Manipulative Physiol Ther. 2008 Sep;31(7):491-502
pubmed: 18803999
J Neurosci. 2011 Jul 27;31(30):10819-28
pubmed: 21795534
Int J Cardiol. 2013 Aug 20;167(4):1159-63
pubmed: 22483260
Ann Neurol. 2011 Aug;70(2):194-206
pubmed: 21674586
Curr Neurovasc Res. 2010 Nov;7(4):301-10
pubmed: 20860549
Mult Scler. 1998 Jun;4(3):99-107
pubmed: 9762655
Neurobiol Dis. 2013 Jun;54:43-58
pubmed: 23466695
Science. 1989 Dec 8;246(4935):1309-12
pubmed: 2479987
PLoS One. 2014 Mar 20;9(3):e89830
pubmed: 24651519
Eur J Neurosci. 1999 Oct;11(10):3648-58
pubmed: 10564372
Spine (Phila Pa 1976). 2013 Oct 15;38(22 Suppl 1):S147-8
pubmed: 23963013
Am J Pathol. 2003 Jul;163(1):57-68
pubmed: 12819011
Neuroscience. 2019 Jul 15;411:177-184
pubmed: 31152935
Cell. 1996 Dec 27;87(7):1161-9
pubmed: 8980223
J Bone Joint Surg Am. 2002 Oct;84(10):1872-81
pubmed: 12377921
Cell Mol Life Sci. 2013 May;70(10):1753-61
pubmed: 23475070
Zhongguo Yi Xue Ke Xue Yuan Xue Bao. 2016 Apr;38(2):150-4
pubmed: 27181890
Biochemistry (Mosc). 2007 Nov;72(11):1194-8
pubmed: 18205601
Proc Natl Acad Sci U S A. 1996 Mar 5;93(5):1988-92
pubmed: 8700872
J Cell Mol Med. 2005 Apr-Jun;9(2):267-85
pubmed: 15963249